Trypanosoma (herpetosoma) Rangeli tejera, 1920: influence of host weight, size of inoculum, and route of infection upon experimental parasitemia

Felix Tejero Natiela Galli Servio Urdaneta-Morales

Abstracts

To study the influence of host age, inoculum size, and route of infection on Trypanosoma (Herpetosoma) rangeli, 12 lots of 6.0 g albino mice (NMRI strain) were infected up. with from 25x10¹ to 25x10(6) trypomastigotes/gram body weight harvested from LIT medium. The lower inocula produced low but persistent parasitemias, while the higher inocula produced high levels of parasitemia that fell quickly, suggesting the mobilization of resistance mechanisms. In other experiments, i.p. inoculation produced higher parasitemias than s.c. inoculation, and 6.0 g mice had higher parasitemias than 16.0 or 26.0 g mice. Thus, a standard methodology would seem to be necessary in the study of the various strains and/or species that may make up the T. rangeli complex.

Trypanosoma (Herpetosoma) rangeli; Inoculum size; Infection route; Host weight; Immune resistance


Para estudiar la influencia de la edad del hosvedador. del tamano del inoculo y su ruta de administración sobre la infection por Trypanosoma (Herpetosoma) rangeli, fueron inoculados i.p. 12 lotes de ratones albinos (cepa NMRI) de 6.0gdepeso con 25.0 - 2.5x10(6) metatripomastigotes/g obtenidos del medio LIT. Los inóculos mas bajos produjeron parasitemias bajas pero persistentes; los inóculos mas elevados originaron niveles altos de parasitemias que cayeron prontamente, sugiriendo la mobilization de mecanismos de resistencia a niveles diferentes de actividad. En otros experimentos, la inoculation i.p. y el uso de ratones de 6.0 g dieron parasitemias mas elevadas que cuando se usaron inoculaciones s.c. o animates de 16.0 y 26.0g. Estos resultados indican la necesidad de emplear una metodologia uniforme cuando se investigue elposible carácter heterogénico del complejo de T. rangeli.


ARTICLES

Trypanosoma (herpetosoma) Rangeli tejera, 1920: influence of host weight, size of inoculum, and route of infection upon experimental parasitemia

Felix Tejero; Natiela Galli; Servio Urdaneta-Morales

Adress to correspondence

ABSTRACT

To study the influence of host age, inoculum size, and route of infection on Trypanosoma (Herpetosoma) rangeli, 12 lots of 6.0 g albino mice (NMRI strain) were infected up. with from 25x101 to 25x106 trypomastigotes/gram body weight harvested from LIT medium. The lower inocula produced low but persistent parasitemias, while the higher inocula produced high levels of parasitemia that fell quickly, suggesting the mobilization of resistance mechanisms. In other experiments, i.p. inoculation produced higher parasitemias than s.c. inoculation, and 6.0 g mice had higher parasitemias than 16.0 or 26.0 g mice. Thus, a standard methodology would seem to be necessary in the study of the various strains and/or species that may make up the T. rangeli complex.

Keywords:Trypanosoma (Herpetosoma) rangeli. Inoculum size. Infection route. Host weight. Immune resistance.

RESUMEN

Para estudiar la influencia de la edad del hosvedador. del tamano del inoculo y su ruta de administración sobre la infection por Trypanosoma (Herpetosoma) rangeli, fueron inoculados i.p. 12 lotes de ratones albinos (cepa NMRI) de 6.0gdepeso con 25.0 - 2.5x106 metatripomastigotes/g obtenidos del medio LIT. Los inóculos mas bajos produjeron parasitemias bajas pero persistentes; los inóculos mas elevados originaron niveles altos de parasitemias que cayeron prontamente, sugiriendo la mobilization de mecanismos de resistencia a niveles diferentes de actividad. En otros experimentos, la inoculation i.p. y el uso de ratones de 6.0 g dieron parasitemias mas elevadas que cuando se usaron inoculaciones s.c. o animates de 16.0 y 26.0g. Estos resultados indican la necesidad de emplear una metodologia uniforme cuando se investigue elposible carácter heterogénico del complejo de T. rangeli.

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REFERENCES

1. Afiez N. Studies on Trypanosoma rangeli Tejera, 1920. 1. Deposition, migration, and growth of T. rangeli in two mammals. Parasitiological Topics, Special Publication No. 1, Society of Protozoologists, p. 19-25, 1981.

2. Ashcroft MT. The relative virulence of Trypanosoma brucei to young and to adult white rats. Annals of Tropical Medicine and Parasitology 53: 89-92, 1959.

3. Brener Z. Contribuição ao estudo da terapêutica experimental da doença de Chagas. Tese. Instituto Nacional de Endemias Rurais, Belo Horizonte, Brasil, 1961.

4. Corson JF. Influence of dose of trypanosomes and of body weight in experimental infections of white rats with Trypanosoma rhodesiense. Annals of Tropical Medicine and Parasitology 28: 525-534,1934.

5. Culbertson JT, Kessler WR. Age resistance of mice to Trypanosoma cruzi. Journal of Parasitology 28: 155- 158, 1942.

6. D'Alessandro A. Biology of Trypanosoma rangeli Tejera, 1920. In: Lumsden WHR, Evans DA (ed.) Biology of the Kinetoplastida, Vol. 1, Academic Press, New York, p. 328-393, 1976.

7. Grewal MS. The life cycle of the British rabbit trypanosome Trypanosoma nabiasi Railliet, 1895. Parasitology 47: 100-118, 1957.

8. Hanson WL. Immune response and mechanisms of resistance in Trypanosoma cruzi. In: Chagas'Disease. Pan-American Health Organization Scientific Publication 347, p. 22-34, 1977.

9. Herbig-Sandreuter A. Further studies on Trypanosoma rangeli Tejera, 1920. ActaTropica 14:193-207,1957. 10. Hoare CA. The Trypanosomes of mammals. Blackwell Scientific Publications. Oxford, 1972.

11. Marsden PD. Trypanosoma cruzi infections in CFI mice. I. Mortality with different doses of trypansomes. Annals of Tropical Medicine and Parasitology 61: 57- 61, 1967.

12. Marsden PD. Trypanosoma cruzi infections in CFI mice. II. Infections induced by different routes. Annals of Tropical Medicine and Parasitology 61: 62-67, 1967.

13. McHardy N. Effect of sex of mice in relation to their response to immunization with vaccines prepared from Trypanosoma cruzi. Transactions of the Royal Society of Tropical Medicine and Hygiene 72: 201-202,1978.

14. Molyneux DH. The morphology and life-history of Trypanosoma (Herpetosoma) microti of the field-vole Microtus agrestis. Annals of Tropical Medicine and Parasitology 63: 229-244, 1969.

15. Molyneux DH. Developmental patterns in trypanosomes of the subgenus Herpetosoma. Annales de la Societe Beige de Medicine Tropicale 50: 229-237, 1970.

16. Mshelbwala AS, Ormerod WE. Measurements of the infectivity of Trypanosoma cruzi in faeces of Rhodnius by comparison of dose-response curves. Journal of General Microbiology 75: 339-350, 1973.

17. Phillips NR. Experimental studies on the quantitative transmission of Trypanosoma cruzi: Considerations regarding the standardization of material. Annals of Tropical Medicine and Parasitology 54: 60-70, 1960.

18. Pifano F. El estado actual de la tripanosomiasis rangeli en Venezuela. Archivos Venezolanos de Medicina Tropical y Parasitologia Medica 5: 185-192, 1973.

19. Silva LH, Nussenzweig V. Sobre uma cepa de Trypanosoma cruzi altamente virulenta para o camundongo branco. Folia Clinica et Biologica 20: 191-207, 1953.

20. Tobie EJ. Experimental transmission and biological comparison of strains of Trypanosoma rangeli. Experimental Parasitology 11: 1-9,1961.

21. Urdaneta-Morales S, Tejero F. Trypanosoma (Herpetosoma) Rangeli tejera, 1920: Mouse model for high, sustained parasitemia. Journal of Parasitology 71:409- 414, 1985.

Recebido para publicação em 3/11/86.

This work was supported by the Consejo de Desarrollo Cientifico y Humanistico de la Universidad Central de Venezuela (Project No. C-03.25/85).

Instituto de Zoologia Tropical, Facultad de Ciencias, Universidad Central de Venezuela.

  • 1. Afiez N. Studies on Trypanosoma rangeli Tejera, 1920.
  • 1. Deposition, migration, and growth of T. rangeli in two mammals. Parasitiological Topics, Special Publication No. 1, Society of Protozoologists, p. 19-25, 1981.
  • 2. Ashcroft MT. The relative virulence of Trypanosoma brucei to young and to adult white rats. Annals of Tropical Medicine and Parasitology 53: 89-92, 1959.
  • 3. Brener Z. Contribuição ao estudo da terapêutica experimental da doença de Chagas. Tese. Instituto Nacional de Endemias Rurais, Belo Horizonte, Brasil, 1961.
  • 4. Corson JF. Influence of dose of trypanosomes and of body weight in experimental infections of white rats with Trypanosoma rhodesiense Annals of Tropical Medicine and Parasitology 28: 525-534,1934.
  • 5. Culbertson JT, Kessler WR. Age resistance of mice to Trypanosoma cruzi Journal of Parasitology 28: 155- 158, 1942.
  • 6. D'Alessandro A. Biology of Trypanosoma rangeli Tejera, 1920. In: Lumsden WHR, Evans DA (ed.) Biology of the Kinetoplastida, Vol. 1, Academic Press, New York, p. 328-393, 1976.
  • 7. Grewal MS. The life cycle of the British rabbit trypanosome Trypanosoma nabiasi Railliet, 1895. Parasitology 47: 100-118, 1957.
  • 8. Hanson WL. Immune response and mechanisms of resistance in Trypanosoma cruzi In: Chagas'Disease. Pan-American Health Organization Scientific Publication 347, p. 22-34, 1977.
  • 9. Herbig-Sandreuter A. Further studies on Trypanosoma rangeli Tejera, 1920. ActaTropica 14:193-207,1957.
  • 10. Hoare CA. The Trypanosomes of mammals. Blackwell Scientific Publications. Oxford, 1972.
  • 11. Marsden PD. Trypanosoma cruzi infections in CFI mice. I. Mortality with different doses of trypansomes. Annals of Tropical Medicine and Parasitology 61: 57- 61, 1967.
  • 12. Marsden PD. Trypanosoma cruzi infections in CFI mice. II. Infections induced by different routes. Annals of Tropical Medicine and Parasitology 61: 62-67, 1967.
  • 13. McHardy N. Effect of sex of mice in relation to their response to immunization with vaccines prepared from Trypanosoma cruzi Transactions of the Royal Society of Tropical Medicine and Hygiene 72: 201-202,1978.
  • 14. Molyneux DH. The morphology and life-history of Trypanosoma (Herpetosoma) microti of the field-vole Microtus agrestis. Annals of Tropical Medicine and Parasitology 63: 229-244, 1969.
  • 15. Molyneux DH. Developmental patterns in trypanosomes of the subgenus Herpetosoma Annales de la Societe Beige de Medicine Tropicale 50: 229-237, 1970.
  • 16. Mshelbwala AS, Ormerod WE. Measurements of the infectivity of Trypanosoma cruzi in faeces of Rhodnius by comparison of dose-response curves. Journal of General Microbiology 75: 339-350, 1973.
  • 17. Phillips NR. Experimental studies on the quantitative transmission of Trypanosoma cruzi: Considerations regarding the standardization of material. Annals of Tropical Medicine and Parasitology 54: 60-70, 1960.
  • 18. Pifano F. El estado actual de la tripanosomiasis rangeli en Venezuela. Archivos Venezolanos de Medicina Tropical y Parasitologia Medica 5: 185-192, 1973.
  • 19. Silva LH, Nussenzweig V. Sobre uma cepa de Trypanosoma cruzi altamente virulenta para o camundongo branco. Folia Clinica et Biologica 20: 191-207, 1953.
  • 20. Tobie EJ. Experimental transmission and biological comparison of strains of Trypanosoma rangeli Experimental Parasitology 11: 1-9,1961.
  • 21. Urdaneta-Morales S, Tejero F. Trypanosoma (Herpetosoma) Rangeli tejera, 1920: Mouse model for high, sustained parasitemia. Journal of Parasitology 71:409- 414, 1985.

  • Adress to correspondence:
    Apartado 47332
    Caracas 1041-A
    VENEZUELA

Publication Dates

  • Publication in this collection
    28 May 2013
  • Date of issue
    Sept 1988

History

  • Accepted
    03 Nov 1986
  • Received
    03 Nov 1986
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