TGF-β1 polymorphism in American tegumentary leishmaniasis in a Southern Brazilian population

Shehadeh, Fabiane Vieira Berg; Santos, Rosiane Ribeiro dos; Silva, Laiane Alzira Denski da; Silva Júnior, Waldir Veríssimo da; Ribas-Silva, Rejane; Ribas, Adriana Danmvolf; Silveira, Thaís Gomes Verzignassi; Borelli, Sueli Donizete; Aristides, Sandra Mara Alessi

doi:10.1590/0037-8682-0415-2017

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Revista da Sociedade Brasileira de Medicina Tropical

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Short Communication • Rev. Soc. Bras. Med. Trop. 52 • 2019 • https://doi.org/10.1590/0037-8682-0415-2017 copy

TGF-β1 polymorphism in American tegumentary leishmaniasis in a Southern Brazilian population

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

INTRODUCTION:

Genetic polymorphisms define the cytokine production leading to susceptibility or resistance to diseases. We studied the cytokine polymorphism in the development of tegumentary leishmaniasis (TL).

METHODS:

Genotyping of TNF-α, TGF-β1, IFN-γ, IL-6, and IL-10 were performed by polymerase chain reaction assay.

RESULTS:

G and C alleles of TGF- β1 (codon 25) were the most common in controls and patients, respectively. G/G was the most frequent genotype in controls, and G/C and C/C in patients.

CONCLUSIONS:

G/G genotype of codon 25 in TGF-β1 appeared to confer resistance, and G/C and C/C genotypes, susceptibility to TL in this population.

Keywords:
Cutaneous leishmaniasis; Genotyping; Gene polymorphisms; Cytokines

Gene (SNP map position)	Allele	Patients (n=102)	Controls (n=150)	p*
TNF-α (-308)	G	180 (88.2%)	260 (86.7%)	0.60
	A	24 (11.8%)	40 (13.3%)
IFN-γ (+874)	T	77 (37.7%)	130 (43.3%)	0.21
	A	127 (62.2%)	170 (56.7%)
IL-6 (-174)	G	132 (64.7%)	212 (70.7%)	0.16
	C	72 (35.3%)	88 (29.3%)
TGF-β1 (codon 10)	T	126 (61.8%)	173 (57.7%)	0.36
	C	78 (38.2%)	127 (42.3%)
TGF-β1 (codon 25)	G	174 (85.3%)	278 (92.7%)	0.01
	C	30 (14.7%)	22 (7.3%)
IL-10 (-1082)	G	74 (36.3%)	109 (36.3%)	0.99
	A	130 (63.7%)	191 (63.7%)
IL-10 (-819)	C	142 (69.6%)	206 (68.7%)	0.82
	T	62 (30.4%)	94 (31.3%)
IL-10 (-592)	A	62 (30.4%)w	94 (31.3%)	0.82
	C	142 (69.6%)	206 (68.7%)

Gene (SNP map position)	Genotype	Patients (n=102)	Controls (n=150)	p*
TNF-α (-308)	G/G	79 (77.4%)	112 (74.7%)	0.90
	G/A	22 (21.6%)	36 (24.0%)
	A/A	1 (1.00%)	2 (1.3%)
IFN-γ (+874)	T/T	15 (14.7%)	33 (22.0%)	0.35
	T/A	47 (46.1%)	64 (42.7%)
	A/A	40 (39.2%)	53 (35.3%)
IL-6 (-174)	G/G	42 (41.2%)	77 (51.3%)	0.285
	G/C	48 (47.1%)	58 (38.7%)
	C/C	12 (11.8%)	15 (10.0%)
TGF-β1 (codon 10)	T/T	41 (40.2%)	48 (32.0%)	0.37
	T/C	44 (43.1%)	77 (51.3%)
	C/C	17 (16.7%)	25 (16.7%)
TGF-β1 (codon 25)	G/G	74 (72.5%)	128 (85.3%)	0.01
	G/C	26 (25.5%)	22 (14.7%)
	C/C	2 (2.0%)	0 (0.0%)
IL-10 (-1082)	G/G	18 (17.6%)	21 (14.0%)	0.47
	G/A	38 (37.2%)	67 (44.7%)
	A/A	46 (45.1%)	62 (41.3%)
IL-10 (-819)	C/C	50 (49.0%)	73 (48.7%)	0.92
	C/T	42 (41.2%)	60 (40.0%)
	T/T	10 (9.8%)	17 (11.3%)
IL-10 (-592)	A/A	10 (9.8%)	17 (11.3%)	0.92
	A/C	42 (41.2%)	60 (40.0%)
	C/C	50 (49.0%)	73 (48.7%)

Gene	Phenotypes	Patients (n=102)	Controls (n=150)	p*
TNF-α	High	23 (22.5%)	38 (25.3%)	0.61
TNF-α	Low	79 (77.4%)	112 (74.7%)	0.61
IFN-γ	High	15 (14.7%)	33 (22.0%)	0.35
	Intermediate	47 (46.1%)	64 (42.7%)
	Low	40 (39.2%)	53 (35.3%)
IL-6	High	90 (88.2%)	135 (90.0%)	0.66
IL-6	Low	12 (11.8%)	15 (10.0%)	0.66
TGF-β1	High	64 (62.7%)	113 (75.3%)	0.08
	Intermediate	30 (29.4%)	27 (18.0%)
	Low	8 (7.8%)	10 (6.7%)
IL-10	High	18 (17.6%)	21 (14.0%)	0.47
	Intermediate	38 (37.2%)	67 (44.7%)
	Low	46 (45.1%)	62 (41.3%)

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[1] Corresponding author: Profa. Sandra Mara Alessi Aristides. e-mail: smaaristides@gmail.com Orcid: 0000-0003-4482-4944