Abstract
INTRODUCTION:
High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins.
METHODS:
Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity.
RESULTS:
Most of the schistosomiasis patient’s (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production.
CONCLUSIONS:
Potato apyrase and SmATPDases have IgE-binding epitopes.
Keywords:
ATP diphosphohydrolase; IgE; Potato apyrase
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