Inserção de DNA de Trypanosoma cruzi no genoma de célula hospedeira de mamífero por meio de infecção

Teixeira, Antonio R.L.; Lacava, Zulmira; Santana, Jaime M.; Luna, Helena

doi:10.1590/S0037-86821991000100010

Resumos

Observamos a cromatina deformas amastigotas de T. cruzi associada a cromossomos de macrófagos metafásicos obtidos em diversos períodos da infecção aguda. O estudo imunocitogenético demonstrou que o material genético inserido naqueles cromossomos era produto do T. cruzi. Pelo teste de hibridizaçâo in situ com sonda biotinilada de DNA de T. cruzi, foi confirmada a inserção de DNA do protozoário nos cromossomos murinos. A inserção seletiva de ³H-DNA do protozoário em alguns cromossomos sugere que podem ocorrer rearranjos transxenogénicos em infecções de mamíferos pelo T. cruzi.

Trypanosoma cruzi; Macrófago; Transxenogene

Trypanosoma cruzi chromatin was observed in macrophage chromosome spreads obtained at different periods of infections in BALB/c mice. Immunofluorescent studies showed that genetic materials associated with the chromosomes were T. cruzi products. In situ hybridization showed the protozoon DNA insertion in the host cell genome. In addition, selective ³H-DNA insertion in chromosomes 3, 6 and 11 was observed, which suggested that transxenogene rearrangement may take place in T. cruzi infections of mammals.

Trypanosoma cruzi; Macrophage; Transxenogene

NOTA PRÉVIA

Inserção de DNA de Trypanosoma cruzi no genoma de célula hospedeira de mamífero por meio de infecção

Antonio R.L. Teixeira; Zulmira Lacava; Jaime M. Santana; Helena Luna

Departamento de Patologia, Faculdade de Ciências da Saúde e Departamentos de Genética e Morfologia e de Biologia Celular, Instituto de Biologia da Universidade de Brasília, Brasilia, DF

^{Endereço para correspondência} Endereço para correspondência: Dr. Antonio R. L. Teixeira. Laboratório de Imunologia/FCS/UnB. Caixa Postal 15 3121, 70910 Brasília, DF.

RESUMO

Observamos a cromatina deformas amastigotas de T. cruzi associada a cromossomos de macrófagos metafásicos obtidos em diversos períodos da infecção aguda. O estudo imunocitogenético demonstrou que o material genético inserido naqueles cromossomos era produto do T. cruzi. Pelo teste de hibridizaçâo in situ com sonda biotinilada de DNA de T. cruzi, foi confirmada a inserção de DNA do protozoário nos cromossomos murinos. A inserção seletiva de ³H-DNA do protozoário em alguns cromossomos sugere que podem ocorrer rearranjos transxenogénicos em infecções de mamíferos pelo T. cruzi.

Palavras-chaves:Trypanosoma cruzi. Macrófago. Transxenogene

SUMMARY

Trypanosoma cruzi chromatin was observed in macrophage chromosome spreads obtained at different periods of infections in BALB/c mice. Immunofluorescent studies showed that genetic materials associated with the chromosomes were T. cruzi products. In situ hybridization showed the protozoon DNA insertion in the host cell genome. In addition, selective³H-DNA insertion in chromosomes 3, 6 and 11 was observed, which suggested that transxenogene rearrangement may take place in T. cruzi infections of mammals.

Keywords:Trypanosoma cruzi. Macrophage. Transxenogene.

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Referências bibliográficas

1. Allshire RC, Dempster M, Hastie ND. Human telomers contain at least three types of G-rich repeat distributted non-randomly. Nucleic Acids Research 17: 4611-4627, 1989.
2. Chess A, Acosta AM, Sethi JK, Santos-Buch GA. Reversible acquisition of a host cell surface antigen by Trypanosoma cruzi. Infection and Immunity 40: 299- 302, 1983.
3. Committee on Standardized Genetic Nomenclature for Mice. Journal of Heredity 63: 69-72, 1972.
4. Djalali M, Adolph S, Steinbach P, Winking H, Hameister H. A comparative mapping study of fragile sites in the human and murine genomes. Human Genetics 77: 157-162, 1987.
5. Harper ME, Sanders GF. Localization of single copy DNA sequences on G-banded chromosomes by in situ hybridization. Chromosoma 83: 431-439, 1981.
6. Rizzo LV, Cunha-NetoE, Teixeira ARL. Autoimmunity in Chagas' disease: specific inhibition of reactivity of CD4+ T cells against myosin in mice chronically infected with Trypanosoma cruzi. Infection and Immunity 57: 2640-2644, 1989.
7. Rudenko G, Van der Ploeg HT. Transcription of telomere repeats in protozoa. EMBO Journal 8:2633-2638, 1989.
8. Searle AG. Mouse chromosome mapping. Genomics 1: 3-18, 1987.
9. Teixeira ARL, Córdoba JC, Souto Maior IC, Solorzano EAC. Chagas' disease: lymphoma growth in rabbits treated with benzonidazole. The American Journal of Tropical Medicine and Hygiene 43: 146-158, 1990.
10. Teixeira ARL, Silva R, Cunha-Neto E, Santana JM, Rizzo LV. Malignant, non-Hodgkin's lymphoma in Trypanosoma cruzi-infected rabbits treated with nitroarenes. Journal of Comparative Pathology 103: 37-48, 1990.
11. Viegas-Pesquignot E, Dutrillaux B, Magdelenat H, Coppey-Moisan M. Mapping of single-copy DNA sequences on human chromosomes by in situ hybridization with byotinilated probes: enhancement of detection sensitivity by intensified-fluorescence digital-imaging microscopy. Proceedings of the National Academy of Sciences of the United States of America 86: 582-586, 1986.
12. Viegas-Pequignot E, Lin LZ, Dutrillaux B, Apiou F, Panlin D. Assignment of human desmin gene to band 2g35 by non-radioacttive in situ hybridization. Human Genetics 83: 33-36, 1989.

Endereço para correspondência:

Dr. Antonio R. L. Teixeira.

Laboratório de Imunologia/FCS/UnB. Caixa Postal 15

3121, 70910 Brasília, DF.

Datas de Publicação

Publicação nesta coleção
27 Maio 2013
Data do Fascículo
Mar 1991

Histórico

Aceito
28 Mar 1991
Recebido
28 Mar 1991

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Allshire RC, Dempster M, Hastie ND. Human telomers contain at least three types of G-rich repeat distributted non-randomly. Nucleic Acids Research 17: 4611-4627, 1989.

[2] 2. Chess A, Acosta AM, Sethi JK, Santos-Buch GA. Reversible acquisition of a host cell surface antigen by Trypanosoma cruzi. Infection and Immunity 40: 299- 302, 1983.

[3] 3. Committee on Standardized Genetic Nomenclature for Mice. Journal of Heredity 63: 69-72, 1972.

[4] 4. Djalali M, Adolph S, Steinbach P, Winking H, Hameister H. A comparative mapping study of fragile sites in the human and murine genomes. Human Genetics 77: 157-162, 1987.

[5] 5. Harper ME, Sanders GF. Localization of single copy DNA sequences on G-banded chromosomes by in situ hybridization. Chromosoma 83: 431-439, 1981.

[6] 6. Rizzo LV, Cunha-NetoE, Teixeira ARL. Autoimmunity in Chagas' disease: specific inhibition of reactivity of CD4+ T cells against myosin in mice chronically infected with Trypanosoma cruzi. Infection and Immunity 57: 2640-2644, 1989.

[7] 7. Rudenko G, Van der Ploeg HT. Transcription of telomere repeats in protozoa. EMBO Journal 8:2633-2638, 1989.

[8] 8. Searle AG. Mouse chromosome mapping. Genomics 1: 3-18, 1987.

[9] 9. Teixeira ARL, Córdoba JC, Souto Maior IC, Solorzano EAC. Chagas' disease: lymphoma growth in rabbits treated with benzonidazole. The American Journal of Tropical Medicine and Hygiene 43: 146-158, 1990.

[10] 10. Teixeira ARL, Silva R, Cunha-Neto E, Santana JM, Rizzo LV. Malignant, non-Hodgkin's lymphoma in Trypanosoma cruzi-infected rabbits treated with nitroarenes. Journal of Comparative Pathology 103: 37-48, 1990.

[11] 11. Viegas-Pesquignot E, Dutrillaux B, Magdelenat H, Coppey-Moisan M. Mapping of single-copy DNA sequences on human chromosomes by in situ hybridization with byotinilated probes: enhancement of detection sensitivity by intensified-fluorescence digital-imaging microscopy. Proceedings of the National Academy of Sciences of the United States of America 86: 582-586, 1986.

[12] 12. Viegas-Pequignot E, Lin LZ, Dutrillaux B, Apiou F, Panlin D. Assignment of human desmin gene to band 2g35 by non-radioacttive in situ hybridization. Human Genetics 83: 33-36, 1989.