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Short Communications • Rev. Soc. Bras. Med. Trop. 49 (05) • Sep-Oct 2016 • https://doi.org/10.1590/0037-8682-0403-2015   copy

Prevalence, and virulence determination of Listeria monocytogenes strains isolated from clinical and non-clinical samples by multiplex polymerase chain reaction

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

INTRODUCTION:

This study aimed to determine the prevalence, and virulence factors of Listeria monocytogenes isolated from various samples by multiplex polymerase chain reaction (MPCR).

METHODS:

A total of 617 isolates were obtained and MPCR was employed for detection of the inlA, inlC, and inlJ genes.

RESULTS:

L. monocytogenes was detected in 46 (7.45%) of the 617 specimens. inlA, inlC, and inlJ were detected in 100%, 76.26%, and 71% isolates, respectively.

CONCLUSIONS:

This study validated MPCR in the analysis and rapid detection of L. monocytogenes. The role of the genes in pathogenesis of the strains can also be affirmed.

Keywords:
Listeria monocytogenes; Virulence genes; Multiplex-PCR

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