Seasonal variation of Lutzomyia longipalpis in Belo Horizonte , State of Minas Gerais Variação sazonal de Lutzomyia longipalpis em Belo Horizonte , Estado de Minas Gerais

Between October, 1997 and September, 1999 in Belo Horizonte, Minas Gerais a study of seasonal variation of Lutzomyia longipalpis was carried out in three distinct areas of the municipality. Sand flies were sampled at 15-day intervals in three residences, in each of which two CDC light traps were installed, one indoors and the other in the peridomicile. A total of 397 sand flies were captured in the three areas, with 65%, 30% and 1% of specimens collected in the eastern, northeast and Barreiro districts, respectively. The overall proportions of sand flies collected inside and around the houses were similar (57% vs 43%) and this pattern was seen for both Lutzomyia longipalpis and Lutzomyia whitmani. The highest population levels during the two years of the study were from October to March. From October onwards, numbers increased constantly until February. A gradual fall was seen from April onwards until the lowest levels were reached in the months of June, July and August. Key-words: Sand fly. Seasonal variation. Control. Leishmaniasis. RESUMO Entre outubro de 1997 e setembro de 1999, em Belo Horizonte, Minas Gerais foi conduzido um estudo de variação sazonal de Lutzomyia longipalpis em três áreas distintas do município. Os flebotomíneos foram coletados quinzenalmente em três residências, em cada área, nas quais foram instaladas duas armadilhas luminosas CDC, sendo uma no intradomicílio e a outra no peridomicílio. Um total de 397 flebotomíneos foi capturado nas três áreas, com 65%, 30% e 1% exemplares coletados nos distritos Leste, Nordeste e Barreiro, respectivamente. A proporção total de flebotomíneos coletados no intradomicílio e peridomicílio foi semelhante (57% vs 43%) e este padrão foi visto para Lutzomyia longipalpis e Lutzomyia whitmani. Durante os dois anos de estudo, o nível da população foi maior no período de outubro a março. A partir de outubro o número de flebotomíneos aumenta constantemente até fevereiro. Uma gradual redução foi observada a partir de abril até alcançar o nível mais baixo nos meses de junho, julho e agosto. Palavras-chaves: Flebotomíneos. Variação sazonal. Controle. Leishmanioses. 1. Fundação Nacional de Saúde, Belo Horizonte, MG. 2. Secretaria Municipal de Saúde de Belo Horizonte, Belo Horizonte, MG. 3. Secretaria Estadual de Saúde de Minas Gerais, Belo Horizonte, MG. Address to: Dr. Marcelo Carvalho de Resende. Rua Rio de Janeiro 1200. Centro, 30160-041 Belo Horizonte, MG. e-mail: marcelo.resende@oi.com.br Recebido para publicação em 14/12/2004 Aceito em 14/11/2004 The incidence of American visceral leishmaniasis (AVL) has increased significantly in some Brazilian cities. In Belo Horizonte, State of Minas Gerais, the process of AVL urbanization began in 1990, and the city still confronts an epidemic state, which demonstrates a worrying geographical advance, with 221 human cases and 23 deaths reported up to 1999. In Minas Gerais, the main vector of Leishmania chagasi, the etiological agent of AVL, is the phlebotomine sand fly Lutzomyia longipalpis. This species has a wide geographical distribution, from Mexico to Argentina and has been collected in all Brazilian states, except those of the southern region, i.e., Paraná, Santa Catarina and Rio Grande do Sul. Despite the medical importance of phlebotomines, information on breeding sites, blood feeding activity, population fluctuations and seasonal variation of these insects is highly variable. This can be attributed to factors inherent to the region, the locality and the observation period.

The incidence of American visceral leishmaniasis (AVL) has increased significantly in some Brazilian cities.In Belo Horizonte, State of Minas Gerais, the process of AVL urbanization began in 1990, and the city still confronts an epidemic state, which demonstrates a worrying geographical advance, with 221 human cases and 23 deaths reported up to 1999.
In Minas Gerais, the main vector of Leishmania chagasi, the etiological agent of AVL, is the phlebotomine sand fly Lutzomyia longipalpis.This species has a wide geographical distribution, from Mexico to Argentina 4 and has been collected in all Brazilian states, except those of the southern region, i.e., Paraná, Santa Catarina and Rio Grande do Sul 9 .
Despite the medical importance of phlebotomines, information on breeding sites, blood feeding activity, population fluctuations and seasonal variation of these insects is highly variable.This can be attributed to factors inherent to the region, the locality and the observation period.
Studies on the duration of phlebotomine generation of under natural conditions revealed that in southern Canada and northern US, the various species of sand flies produce a single annual generation and can remain in diapause during the winter months 6 .In Minas Gerais, L. longipalpis appears to produce about four generations per year, with a threemonth interval between generations, this interval corresponds to the incubation period of L. chagasi in the dogs, the main domestic reservoir of the protozoan 9 .
Considering AVL's morbidity and mortality, disease control should be prioritized in actions of epidemiological surveillance.
Thus, studies of the seasonal variation of L. longipalpis become necessary, since the data obtained can support a program of integrated control, to determine the most favorable times of the year to apply insecticides, with a view to the rational utilization of resources and a reduction in environmental damage as a result of this zoonosis control.In this study, the seasonal variation of L. longipalpis in three areas of the municipality of Belo Horizonte was investigated.

MATERIAL AND METHODS
The City of Belo Horizonte, in the State of Minas Gerais, Brazil, has a population of 3,420,000 inhabitants and the greater part of the population live in substandard accommodations, without basic sanitation and hygiene conditions.Belo Horizonte is formed by nine regional areas: Barreiro, Centro Sul (Center-South), Leste (East), Nordeste (Northeast), Noroeste (Northwest), Norte (North), Oeste (West), Pampulha and Venda Nova.We selected the regions Barreiro, Leste (East) and Noroeste (Northeast) due to orientation by the Municipal Health Secretary of Belo Horizonte, through which data on AVL prevalence in dogs and humans was assessed.
The study was carried out for over 24 months, between October, 1997 and September, 1999, in three distinct areas belonging to the municipality of Belo Horizonte: i.e., the East (E) and Northeast (NE) districts (areas with a high prevalence of canine AVL and the occurrence of human cases) and the Barreiro district, where the canine AVL prevalence is low and human cases have not been reported.
Three residences were chosen from each region totaling 9 houses for insect collection and the selected residences signed a consent term in order to assure their participation and collaboration during the period of the study.The houses under study presented a favorable environment for the reproduction and development of sand flies.In general, the houses had the following features: large back yards, plants and domestic animals (dogs, birds, chickens, and others).Phlebotomines were sampled at 15-day intervals in three residences, in each of them two CDC light traps were installed, one indoors and the other one in the peridomicile.
The traps were hung 80cm above the ground, operated between 5:00 PM and 6:00 PM and collected between 07:30 AM and 08:30 AM the next morning.The samples were sent to laboratory of the Núcleo de Entomologia of Fundação Nacional de Saúde (FUNASA) of Minas Gerais, where the specimens were separated, prepared and identified according to Young & Duncan 14 .

RESULTS
Sampling of phlebotomines by district.The total number of phlebotomines and the number of L. longipalpis collected in the E, NE and Barreiro districts of Belo Horizonte between October, 1997 and September 1999 are shown in Table 1.In general terms, the number of collected sand flies and L. longipalpis was shown to be higher in the year I than year II.A total of 397 sand flies were captured in the three areas with 275 (69%), 118 (30%) and 4 (1%) specimens collected in the E, NE and Barreiro districts, respectively.L. longipalpis was not found in the Barreiro district and, although lower numbers of this species were collected in the NE than the E, the highest proportion (62.7%) of all sand flies were captured in the former rather than in the latter regions.Distribution of phlebotomines by species and gender.The total numbers and relative proportions of each gender of the phlebotomine species collected in the three districts of Belo Horizonte are presented in Table 2.A similar proportion of male (55%) and female (45%) sand flies were collected during the 24-month study, all 10 (ten) species belonging to the genus Lutzomyia (França, 1924).The predominant species were: Lutzomyia longipalpis, 156 (39%) specimens, L. whitmani, 150 (38%), L. monticola, 55 (14%) and L. sallesi, 24(6%) specimens.The other species found in the municipality represented only 3% of the total collected and included L. intermedia, L. lenti, L. lloydi, L. edwardsi, L. evandroi and L. migonei.Three of these species i.e., L. intermedia, L. whitmani and L. migonei have been incriminated as vectors of L. braziliensis, the etiological agent of American cutaneous leishmaniasis (ACL).
Frequency of phlebotomines by habitat sampled.The relative distribution of the 10 phlebotomine species in two habitats (intradomiciliary and peridomiciliary) during the study period are shown in Table 3.The overall proportions of sand flies collected inside and around the houses were fairly similar (57% vs 43%) and this pattern was seen for both L. longipalpis and L. whitmani.Only one of the species collected in Monthly distribution of phlebotomines.The monthly distribution of sand flies captured in the three districts of Belo Horizonte, is presented in Figure 1, together with the mean monthly rainfall (mm) and minimum and maximum monthly temperatures (ºC).The numbers of phlebotomines, in general and L. longipalpis, in particular, were greater when the combination of the highest mean temperatures and regular rainfall occurred.Sand fly populations reach their lowest levels in Belo Horizonte during the coldest and driest months.
The highest population levels during the two years of the study from October to March.From October onwards, the numbers increased constantly until February.A gradual fall was observed from April onwards until the lowest levels were reached in the months of June, July and August.

Figure 1 -
Figure 1 -Monthly distribution of phlebotomines in Belo Horizonte, together with mean monthly rainfall (mm) and minimum and maximum temperatures (ºC) from October 1997 to September 1999.