INTRODUCTION: The aim of this study was detect the presence of human papillomavirus and investigate the prevalence and distribution of the HPV-6, 11, 16 and 18 genotypes among HIV-1 positive and negative women. METHODS: Samples of cervical smears from 98 women were analyzed using the nested polymerase chain reaction for the presence of HPV and using the type-specific technique for genotype detection. Genotypes were confirmed by means of restriction fragment analysis. The chi-square and Fisher tests were performed for the statistical analysis. RESULTS: HPV-DNA was observed in 66.3% of the samples analyzed: 76.4% in the HIV-positive group and 60% in the HIV-negative group (p = 0.1). Higher prevalence of viral infection due to oncogenic genotypes was observed in the HIV-positive group (65.2%) than in the HIV-negative group (28.6%), (p = 0.006), and HPV-16 was the most frequent in the two groups, followed by HPV-18. CONCLUSIONS: It is suggested that HIV-positive women present a higher likelihood of infection by oncogenic HPV genotypes, and the importance of a screening and differential diagnosis program for this group is highlighted.
Human papillomavirus; HIV; Cervical cancer; Polymerase chain reaction
