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Phenotypic and genetic variation of Triatoma costalimai (Hemiptera: Reduviidae)

Abstract

INTRODUCTION:

We aimed to study intraspecific variation in Triatoma costalimai, a potential vector of Chagas disease present in Brazil and Bolivia.

METHODS:

We analyzed phenotypic (connexivum color patterns, wing morphometrics) and genetic variation (16S mtDNA) of three Brazilian T. costalimai populations. We compared 16S sequences with those of putative Bolivian T. costalimai and its sister species, T. jatai.

RESULTS:

Brazilian populations had different connexivum color patterns and forewing shapes. A 16S mtDNA haplotype network showed a clear separation of Brazilian T. costalimai from both T. jatai and Bolivian T. costalimai.

CONCLUSIONS:

We report considerable variability in T. costalimai populations.

Keywords:
Triatoma costalimai; 16S mtDNA; Connexivum color patterns; Wing morphometry; Chagas disease

There are approximately 150 known triatomine species11. Monteiro FA, Weirauch C, Felix M, Lazoski C, Abad-Franch F. Evolution, systematics, and biogeography of the Triatominae, vectors of Chagas disease. Adv Parasitol. 2018;99:265-344.. Almost half of these species are found in Brazil, with at least 25 species being native to the Cerrado savanna22. Galvão C, Gurgel-Gonçalves R. Vetores conhecidos no Brasil. In: Vetores da doença de Chagas no Brasil. Série Zoológica: Guias e Manuais de identificação. Curitiba, 2015, 88-170.. Triatoma costalimai Verano & Galvão (1958) and Triatoma jatai Gonçalves, Teves-Neves, Santos-Mallet, Carbajal-de-la-Fuente and Lopes (2013) are rock-dwelling species apparently endemic to the Brazilian savanna33. Verano OT, Galvão AB. Triatoma costalimai sp.n. Rev Bras Malariol D Trop. 1958; 10:199-205.,44. Gonçalves TCM, Teves-Neves SC, Santos-Mallet JRD, Carbajal-De-La-Fuente AL, Lopes CM. Triatoma jatai sp. in the state of Tocantins, Brazil (Hemiptera: Reduviidae: Triatominae). Mem Inst Oswaldo Cruz. 2013;108(4):429-37.. Triatoma costalimai has been reported in the states of Bahia, Goiás, Minas Gerais, and Tocantins22. Galvão C, Gurgel-Gonçalves R. Vetores conhecidos no Brasil. In: Vetores da doença de Chagas no Brasil. Série Zoológica: Guias e Manuais de identificação. Curitiba, 2015, 88-170.,55. Machiner F, Cardoso RM, Castro C, Gurgel-Gonçalves R. Occurrence of Triatoma costalimai (Hemiptera: Reduviidae) in different environments and climatic seasons: a field study in the Brazilian savanna. Rev Soc Bras Med Trop. 2012; 45(5):567-71.. In the wild, T. costalimai occupies limestone outcrops in association with rodents, birds, and squamate reptiles; however, the species also invades and colonizes houses and peri-domestic structures in Goiás and Tocantins66. Brito RN, Diotaiuti L, Gomes ACF, Souza RCM, Abad-Franch F. Triatoma costalimai (Hemiptera: Reduviidae) in and around houses of Tocantins State, Brazil, 2005-2014. J Med Entomol. 2017;54(6):1771-4.. Trypanosoma cruzi infection in T. costalimai may vary from 13.7% (n = 839) in specimens caught in and around houses66. Brito RN, Diotaiuti L, Gomes ACF, Souza RCM, Abad-Franch F. Triatoma costalimai (Hemiptera: Reduviidae) in and around houses of Tocantins State, Brazil, 2005-2014. J Med Entomol. 2017;54(6):1771-4. to 64.2% (n = 53) in specimens caught in periurban rock outcroppings77. Teves SC, Toma HK, Lopes CM, Oliveira BLN, Carbajal-de-la-Fuente AL, Souza DM et al. Triatoma costalimai naturally infected by Trypanosoma cruzi: a public health concern. Am J Trop Med Hyg. 2019;100(1):90-2.. These data indicate that T. costalimai is a potential vector of Chagas disease. The closely related and morphologically similar T. jatai has so far only been reported in southeastern Tocantins, where wild populations are found in rock outcrops and house-invading specimens have been reported sporadically. Triatoma jatai differs from T. costalimai in color, wing size, and external structures of the male genitalia44. Gonçalves TCM, Teves-Neves SC, Santos-Mallet JRD, Carbajal-De-La-Fuente AL, Lopes CM. Triatoma jatai sp. in the state of Tocantins, Brazil (Hemiptera: Reduviidae: Triatominae). Mem Inst Oswaldo Cruz. 2013;108(4):429-37.. The analysis of their two mtDNA gene fragments (16S and COI) supports the hypothesis that T. jatai is a sister species of T. costalimai88. Teves SC, Gardim S, Carbajal De La Fuente AL, Lopes CM, Goncalves TC, Dos Santos JRM et al. Mitochondrial genes reveal Triatoma jatai as a sister species to Triatoma costalimai (Reduviidae: Triatominae). Am J Trop Med Hyg . 2016;94(3):686-8..

Triatoma costalimai has also been reported in Cochabamba, Bolivia99. Justi SA, Russo CA, Dos Santos JRM, Obara MT, Galvão C. Molecular phylogeny of Triatomini (Hemiptera: Reduviidae: Triatominae). Parasit Vectors. 2014;7:149.. In addition, we collected T. costalimai specimens from the Brazilian states of Goiás and Bahia that had different connexivum color patterns1010. Lent H, Wygodzinsky P. Revision of the Triatominae (Hemiptera, Reduviidae), and their significance as vectors of Chagas' disease. Bull Am Mus Natl Hist. 1979;163:123-520.. Here, we aimed to investigate intraspecific variation in T. costalimai by analyzing phenotypic (connexivum color patterns, wing morphometrics) and genetic data (mtDNA 16S sequences) of populations from Brazil (Bahia plus central and northeast Goiás) and Bolivia.

We sampled T. costalimai between 2014 and 2016 in three municipalities: Carmo do Rio Verde, Posse, and São Desidério (see geographical information in the Supplementary Material, Table 1). In each municipality, we collected triatomines manually using forceps over three consecutive days. In São Desidério, we complemented daytime collections with two collections three hours after dusk to increase the number of specimens. The presence of T. cruzi-infected specimens of T. costalimai was observed in São Desidério (DPV, personal communication). We defined wild environments as rocky outcrops located more than 300 meters from the nearest human dwelling. We identified triatomines according to Lent & Wygodzinsky1010. Lent H, Wygodzinsky P. Revision of the Triatominae (Hemiptera, Reduviidae), and their significance as vectors of Chagas' disease. Bull Am Mus Natl Hist. 1979;163:123-520. and Gonçalves et al.44. Gonçalves TCM, Teves-Neves SC, Santos-Mallet JRD, Carbajal-De-La-Fuente AL, Lopes CM. Triatoma jatai sp. in the state of Tocantins, Brazil (Hemiptera: Reduviidae: Triatominae). Mem Inst Oswaldo Cruz. 2013;108(4):429-37.

Right forewings were mounted using Canada Balsam between microscope slides and cover slips and photographed at 8x magnification with a Leica M205C confocal system. The number of specimens used is shown in the Supplementary Material. We used tpsDig to digitize nine anatomical landmarks (1, 2, 3, 5, 6, 7, 9, 10, and 13 of Schachter-Broide et al.1111. Schachter-Broide J, Dujardin JP, Kitron U, Gürtler RE. Spatial structuring of Triatoma infestans (Hemiptera, Reduviidae) populations from northwestern Argentina using wing geometric morphometry. J Med Entomol . 2004;41(4):643-9.). Shape and size variables were computed using the tpsRelw version 1.18. We used analysis of variance (ANOVA) of centroid size (CS) to compare forewing size across populations. Forewing shape variation was investigated by canonical variate analysis using the MorphoJ software version 2.0. We assessed the influence of size on wing shape (allometry) by multiple regression, using Procrustes coordinates as the dependent variable and CS as the independent variable.

We extracted DNA from the abdominal tissue of each specimen using the Qiagen DNeasy Blood and Tissue Kit (Qiagen, Crawley, United Kingdom), following the manufacturer’s protocol. Two adult T. costalimai specimens from the municipality of Mambaí, state of Goiás55. Machiner F, Cardoso RM, Castro C, Gurgel-Gonçalves R. Occurrence of Triatoma costalimai (Hemiptera: Reduviidae) in different environments and climatic seasons: a field study in the Brazilian savanna. Rev Soc Bras Med Trop. 2012; 45(5):567-71., were included to increase the number of sequences analyzed. DNA was amplified using polymerase chain reaction with the 16S primers described by Lyman et al.1212. Lyman DF, Monteiro FA, Escalante AA, Cordon-Rosales C, Wesson DM, Dujardin JP, Beard CB. Mitochondrial DNA sequence variation among triatomine vectors of Chagas' disease. Am J Trop Med Hyg .1999;60(3):377-86.. Reactions were carried out in a MyCycler thermal cycler (Bio-Rad, Hercules, CA, USA) with 1 μL of template DNA, 4 μL of 5× FIREpol Master mix, 1 μL of primers (10 mM), and 13 μL of ultrapure water. Sequencing reactions (forward and reverse) were carried out with ABI Prism dGTP Big Dye Terminator v3.1 kits (Applied Biosystems, Foster City, CA, USA) and sequenced on a Hitachi/ABI PRISM 3100 Genetic Analyzer/DNA Sequencer at Rede Premium, Faculty of Medicine, University of São Paulo, Brazil. We analyzed the sequences in Chromas version 1.45. When more than two sequences were obtained from a given sampled site, we used DNAsp version 5 to determine the number of haplotypes present in specimens from the site. Genetic relationships between haplotypes were inferred using a parsimonious median-joining network with the software Network 5.0.0.3. We assembled a distance matrix with these sequences using the Kimura 2-parameter model to assess the genetic distance between sequences. We generated the matrix in MEGA 6 and used triatomine sequences from GenBank for the analysis, as shown in the supplemental material. Reference specimens were deposited in the Entomological Collection of the Faculty of Public Health, University of São Paulo, under the following registration numbers: specimens from Posse, northeastern Goiás, E15668 to E15671; specimens from Carmo do Rio Verde, central Goiás, E15672, and E15673; and specimens from São Desidério, Bahia, E15674 to E15677.

We found that specimens from Carmo do Rio Verde (central Goiás) have a typical connexivum color pattern that is similar to that described in Lent and Wygodzinsky1010. Lent H, Wygodzinsky P. Revision of the Triatominae (Hemiptera, Reduviidae), and their significance as vectors of Chagas' disease. Bull Am Mus Natl Hist. 1979;163:123-520.(see Figure 56, p. 217), with orange-red markings of variable width extending along the outer connexivum border and the dark, inner portion of the connexivum advancing towards the external border along the intersegmental sutures. However, specimens from São Desidério (Bahia) and Posse (northeastern Goiás) have continuous orange-red markings along the outer edge of the connexivum (Figure 1A), a pattern that resembles the one depicted in Figure 58 (p. 220) of Lent and Wygodzinsky1010. Lent H, Wygodzinsky P. Revision of the Triatominae (Hemiptera, Reduviidae), and their significance as vectors of Chagas' disease. Bull Am Mus Natl Hist. 1979;163:123-520..

The mean forewing CS of the Carmo do Rio Verde population (2.67 ± 1.26 mm, SD) was smaller than that of specimens from São Desidério (2.83 ± 1.76 mm) and Posse (2.87 ± 1.33 mm). ANOVA showed a significant difference in wing size (F = 3.482, df = 2, p = 0.04514). Factorial maps of forewing shape variation revealed differences among populations; CV1 distinguished specimens from Carmo do Rio Verde from those caught in São Desidério and Posse (Figure 1B). Regression analysis between shape and size was not statistically significant that revealed absence of allometric effect.

FIGURE 1:
A. Connexivum color patterns found in Triatoma costalimai specimens. B. Scatterplot of the first two canonical variate scores for wing shape variation in Triatoma costalimai populations. The percentage contribution of each component to the total shape variation is shown in parentheses on the axes. The drawings show the changes in shape at the extremities of the axes.

Thirty-four 16S sequences of 403 bp were obtained: 24 from Carmo do Rio Verde specimens (two haplotypes: CRV1 and CRV2), six from São Desidério specimens (two haplotypes: SD1 and SD2), two from Mambaí (two haplotypes: Mambaí 1 and 2), and two from Posse (one haplotype: P9/P10). A median-joining network with 16S sequences of Brazilian and Bolivian T. costalimai and that from T. jatai showed clear differences among these groups (Figure 2). The genetic distance between T. costalimai sequences from Brazil (Table 1) varied from 0.002 to 0.023; however, the distance between the sequences and the putative T. costalimai sequence from Bolivia was greater (0.028 to 0.041). Moreover, the distance between T. costalimai and T. jatai from Brazil varied from 0.023 to 0.031.

FIGURE 2:
Median-joining network of haplotypes based on T. costalimai 16S sequences. Red dots are median vectors, a possible ancestral sequence. CRV1 and CRV2: T. costalimai from Carmo do Rio Verde. SD1 and SD2: T. costalimai from São Desidério; Mambai: T. costalimai from Mambaí; Posse: T. costalimai from Posse; TO: T. costalimai from Tocantins; Bol: Triatoma aff. costalimai from Bolivia; T. jatai: Triatoma jatai from Tocantins.

TABLE 1:
Genetic distance matrix for 16S mtDNA sequences of Triatoma costalimai, T. jatai, and the Bolivian specimen identified as T. costalimai. Analyses were conducted using the Kimura 2-parameter model.

Triatoma costalimai showed considerable phenotypic and genetic variation in Brazil. A 16S mtDNA haplotype network showed a clear separation of Brazilian T. costalimai from both T. jatai and Bolivian specimens identified as T. costalimai. The connexival segments of T. costalimai from central Goiás have orange-red markings of variable widths extending along the outer connexival border, and the pattern is similar to that described by Lent & Wygodzinsky (Figure 56; p. 217)1010. Lent H, Wygodzinsky P. Revision of the Triatominae (Hemiptera, Reduviidae), and their significance as vectors of Chagas' disease. Bull Am Mus Natl Hist. 1979;163:123-520. and Verano & Galvão33. Verano OT, Galvão AB. Triatoma costalimai sp.n. Rev Bras Malariol D Trop. 1958; 10:199-205.. However, specimens from Bahia and Northeastern Goiás have continuous markings along the outer edge of the connexival border (Figure 58; p. 220 of Lent & Wygodzinsky1010. Lent H, Wygodzinsky P. Revision of the Triatominae (Hemiptera, Reduviidae), and their significance as vectors of Chagas' disease. Bull Am Mus Natl Hist. 1979;163:123-520.). Moreover, these patterns are different from the connexivum of T. jatai, which has yellow spots above the intersegmental suture44. Gonçalves TCM, Teves-Neves SC, Santos-Mallet JRD, Carbajal-De-La-Fuente AL, Lopes CM. Triatoma jatai sp. in the state of Tocantins, Brazil (Hemiptera: Reduviidae: Triatominae). Mem Inst Oswaldo Cruz. 2013;108(4):429-37.. Triatomines have high morphological plasticity and closely related species develop rapid morphological variations as they adapt to different environments1313. Dujardin JP, Panzera P, Schofield CJ. Triatominae as a model of morphological plasticity under ecological pressure. Mem Inst Oswaldo Cruz . 1999;94(Suppl 1):223-8.. We suggest that the connexivum color patterns are related to environmental conditions where the triatomines were collected from, and this has been previously observed in T. patagonica1414. Nattero J, Pita S, Calleros L, Crocco L, Panzera Y, Rodriguez CS et al. Morphological and genetic differentiation within the southernmost vector of Chagas Disease: Triatoma patagonica (Hemiptera - Reduviidae). PLoS One. 2016;1:e0168853.. Wing morphometry also revealed differences within T. costalimai populations and between T. jatai and T. costalimai44. Gonçalves TCM, Teves-Neves SC, Santos-Mallet JRD, Carbajal-De-La-Fuente AL, Lopes CM. Triatoma jatai sp. in the state of Tocantins, Brazil (Hemiptera: Reduviidae: Triatominae). Mem Inst Oswaldo Cruz. 2013;108(4):429-37.. The analyses of wing traits may discriminate sibling taxa and reveal fine-scale spatial structuring among populations of a single species1111. Schachter-Broide J, Dujardin JP, Kitron U, Gürtler RE. Spatial structuring of Triatoma infestans (Hemiptera, Reduviidae) populations from northwestern Argentina using wing geometric morphometry. J Med Entomol . 2004;41(4):643-9..

The phenotypic plasticity of Triatominae sometimes leads to misidentification of genetically distinct convergent species particularly through the qualitative evaluation of chromatic characters1515. Monteiro FA, Escalante AA, Beard CB. Molecular tools and triatomine systematics: a public health perspective. Trends Parasitol. 2001;17(7):344-7.. These mistakes could be consequences of the incorrect and exclusive use of the dichotomous keys of Lent & Wygodzinsky1010. Lent H, Wygodzinsky P. Revision of the Triatominae (Hemiptera, Reduviidae), and their significance as vectors of Chagas' disease. Bull Am Mus Natl Hist. 1979;163:123-520. that disregard the ecological and geographic characteristics of the specimens especially when dealing with phenotypic variation or cryptic species. This seems to be the case for the specimens morphologically similar to T. costalimai collected in Bolivia. Incorrect identification might have both systematic and epidemiological implications as taxonomic uncertainties could generate misleading occurrence records that could result in biogeographic inference mistakes1515. Monteiro FA, Escalante AA, Beard CB. Molecular tools and triatomine systematics: a public health perspective. Trends Parasitol. 2001;17(7):344-7..

Although there is no consensus regarding the similarity or difference values of different genetic markers to define a new species of Triatominae, our study revealed a clear genetic distance of 0.041 between the Bolivian and the Brazilian T. costalimai based on the analysis of 16S sequences. Analysis by Teves et al.88. Teves SC, Gardim S, Carbajal De La Fuente AL, Lopes CM, Goncalves TC, Dos Santos JRM et al. Mitochondrial genes reveal Triatoma jatai as a sister species to Triatoma costalimai (Reduviidae: Triatominae). Am J Trop Med Hyg . 2016;94(3):686-8. revealed a distance of 0.025 between T. jatai and T. costalimai collected in Tocantins based on the analysis of 16S sequences. We report considerable variability in Brazilian T. costalimai populations. Moreover, we found substantial mtDNA divergence between the bona fide Brazilian T. costalimai and the Bolivian specimen identified as T. costalimai. Further studies and the inclusion of new populations are necessary to suggest a new Triatoma species from Bolivia.

ACKNOWLEDGEMENTS

We are indebted to Secretaria Municipal de Saúde de Carmo do Rio Verde, Goiás, Secretaria Estadual de Saúde da Bahia and Secretaria Estadual de Saúde de Minas Gerais, who kindly helped us with field collections. We also thank Fernando Abad-Franch for comments on the final version of the manuscript.

REFERENCES

  • 1
    Monteiro FA, Weirauch C, Felix M, Lazoski C, Abad-Franch F. Evolution, systematics, and biogeography of the Triatominae, vectors of Chagas disease. Adv Parasitol. 2018;99:265-344.
  • 2
    Galvão C, Gurgel-Gonçalves R. Vetores conhecidos no Brasil. In: Vetores da doença de Chagas no Brasil. Série Zoológica: Guias e Manuais de identificação. Curitiba, 2015, 88-170.
  • 3
    Verano OT, Galvão AB. Triatoma costalimai sp.n. Rev Bras Malariol D Trop. 1958; 10:199-205.
  • 4
    Gonçalves TCM, Teves-Neves SC, Santos-Mallet JRD, Carbajal-De-La-Fuente AL, Lopes CM. Triatoma jatai sp. in the state of Tocantins, Brazil (Hemiptera: Reduviidae: Triatominae). Mem Inst Oswaldo Cruz. 2013;108(4):429-37.
  • 5
    Machiner F, Cardoso RM, Castro C, Gurgel-Gonçalves R. Occurrence of Triatoma costalimai (Hemiptera: Reduviidae) in different environments and climatic seasons: a field study in the Brazilian savanna. Rev Soc Bras Med Trop. 2012; 45(5):567-71.
  • 6
    Brito RN, Diotaiuti L, Gomes ACF, Souza RCM, Abad-Franch F. Triatoma costalimai (Hemiptera: Reduviidae) in and around houses of Tocantins State, Brazil, 2005-2014. J Med Entomol. 2017;54(6):1771-4.
  • 7
    Teves SC, Toma HK, Lopes CM, Oliveira BLN, Carbajal-de-la-Fuente AL, Souza DM et al. Triatoma costalimai naturally infected by Trypanosoma cruzi: a public health concern. Am J Trop Med Hyg. 2019;100(1):90-2.
  • 8
    Teves SC, Gardim S, Carbajal De La Fuente AL, Lopes CM, Goncalves TC, Dos Santos JRM et al. Mitochondrial genes reveal Triatoma jatai as a sister species to Triatoma costalimai (Reduviidae: Triatominae). Am J Trop Med Hyg . 2016;94(3):686-8.
  • 9
    Justi SA, Russo CA, Dos Santos JRM, Obara MT, Galvão C. Molecular phylogeny of Triatomini (Hemiptera: Reduviidae: Triatominae). Parasit Vectors. 2014;7:149.
  • 10
    Lent H, Wygodzinsky P. Revision of the Triatominae (Hemiptera, Reduviidae), and their significance as vectors of Chagas' disease. Bull Am Mus Natl Hist. 1979;163:123-520.
  • 11
    Schachter-Broide J, Dujardin JP, Kitron U, Gürtler RE. Spatial structuring of Triatoma infestans (Hemiptera, Reduviidae) populations from northwestern Argentina using wing geometric morphometry. J Med Entomol . 2004;41(4):643-9.
  • 12
    Lyman DF, Monteiro FA, Escalante AA, Cordon-Rosales C, Wesson DM, Dujardin JP, Beard CB. Mitochondrial DNA sequence variation among triatomine vectors of Chagas' disease. Am J Trop Med Hyg .1999;60(3):377-86.
  • 13
    Dujardin JP, Panzera P, Schofield CJ. Triatominae as a model of morphological plasticity under ecological pressure. Mem Inst Oswaldo Cruz . 1999;94(Suppl 1):223-8.
  • 14
    Nattero J, Pita S, Calleros L, Crocco L, Panzera Y, Rodriguez CS et al. Morphological and genetic differentiation within the southernmost vector of Chagas Disease: Triatoma patagonica (Hemiptera - Reduviidae). PLoS One. 2016;1:e0168853.
  • 15
    Monteiro FA, Escalante AA, Beard CB. Molecular tools and triatomine systematics: a public health perspective. Trends Parasitol. 2001;17(7):344-7.
  • Financial Support: Fundação de Amparo à Pesquisa do Estado de São Paulo. DPV is the recipient of a PhD fellowship from FAPESP (2012/19238-4).

Publication Dates

  • Publication in this collection
    21 Dec 2020
  • Date of issue
    2021

History

  • Received
    05 Feb 2020
  • Accepted
    13 July 2020
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