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Short Communications • Rev. Soc. Bras. Med. Trop. 48 (2) • mar-apr 2015 • https://doi.org/10.1590/0037-8682-0199-2014   copy

A rapid and simple method to detect ESBL in Enterobacter cloacae based on MIC of cefepime

Authorship SCIMAGO INSTITUTIONS RANKINGS

INTRODUCTION:

The aim of this study was to identify a rapid and simple phenotypic method for extended-spectrum β-lactamase (ESBL) detection in Enterobacter cloacae.

METHODS:

A total of 79 consecutive, non-repeated samples of E. cloacae were evaluated. Four phenotypic methods were applied for ESBL detection, results were compared to multiplex polymerase chain reaction (PCR) as the gold standard reference method: 1) ceftazidime and cefotaxime disks with and without clavulanate, both with boronic acid added; 2) disk approximation using cefepime and amoxicillin/clavulanate; 3) ESBL screening by minimum inhibitory concentration (MIC) ≥ 16µg/mL and 4) by MIC ≥ 2µg/mL for cefepime.

RESULTS:

Method 4 showed the best combination of sensitivity (100%) and specificity (94%).

CONCLUSIONS:

MIC ≥ 2µg/mL for cefepime would be very useful for the phenotypic detection of ESBL in samples of E. cloacae.

Enterobacter cloacae; ESBL; Phenotypic detection

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