Note ATTRACTION OF Astylus variegatus ( GERM . ) ( COLEOPTERA : MELYRIDAE ) BY VOLATILE FLORAL ATTRACTANTS

The beetle Astylus variegatus (Germ.) (Coleoptera: Melyridae) is frequently found in flowers feeding on pollen. Responses of A. variegatus to volatile floral attractants were studied in common beans (Phaseolus vulgaris L.) fields. Traps originally designed to capture Diabrotica speciosa (Germ.) (Coleoptera: Chrysomelidae), consisted of plastic bottles (2 L) with 150 holes (5-mm diameter) yellow gold painted and containing inside a plastic strip (3.5 × 25 cm) with Lagenaria vulgaris (L.) powder (0.28% B cucurbitacin feeding stimulant and arrestant for diabroticites) sprayed with carbaril insecticide. Treatments consisted of 1,4-dimethoxybenzene (one or two dispensers per trap), 1,4dimethoxybenze + indole, 1,4-dimethoxybenzene + cinnameldehyde and control. Volatile average release rates (over ten days) was approximately 32 mg day per dispenser under laboratory conditions. 1,4dimethoxybenzene-lured traps caught significantly more beetles than the control, three and seven days after trap setting. Ten days after the onset of the experiment, there were no differences in number of beetles caught by treatments. Captures were higher in the 1,4-dimethoxybenzene + cinnamaldehyde treatment than in 1,4-dimethoxybenzene only in the first assessment. Adding indole to 1,4dimethoxybenzene did not improve beetle captures.


INTRODUCTION
Melyridae are small to medium size beetles that feed on pollen (Willemstein, 1987).Astylus variegatus (Germ.)(Coleoptera: Melyridae) is a univoltine beetle associated with flowers of native and exotic plants in South America (Matioli et al., 1990).
The life cycle lasts about one year and the larvae live in the soil.Under favorable humidity conditions (dry season), the larvae damage seeds and seedlings mostly in corn (Bianco, 1991).Therapeutic tactics are not available for larval management; seed treatment with insecticides is the suggested measure to control them (Bianco, 1991).
Bright yellow is reported as attractive to A. variegatus beetles (Matioli & Figueira, 1988;Gonçalves, 1997).Several yellow traps attract and capture A. variegatus (Matioli et al., 1990).From 1997 to 2003, a series of field experiments on the chemical ecology of D. speciosa and C. arcuata tingomariana (Ventura et al., 2000;2001) were conducted and yellow traps caught non-target A. variegatus in some experiments.The responses of A. variegastus beetles to volatile floral attractants were studied in the field.

MATERIAL AND METHODS
A field assay was conducted in 2003 to evaluate the volatile dispenser ability to attract Diabrotica speciosa (Germ.)(Coleoptera: Chrysomelidae) beetles.The experiment also aimed to assess the effect of adding 1,4-dimetoxybenze and trans-cinnamaldeyde to 1,4dimetoxybenzene on captures.The experiment was carried out in Londrina (23 o 19'S, 51 o 12'W), PR, Brazil.Common beans, Phaseolus vulgaris L., cv.IAPAR 59, sown on January 15, 2003 were used as testing sites.The traps consisted of plastic bottles (2 L) with 150 holes (5-mm diameter) yellow gold painted having inside a plastic strip (3.5 × 25.0 cm) containing Lagenaria vulgaris (L.) powder (0.28% cucurbitacin) -a feeding stimulant and arrestant for diabroticitessprayed with carbaril insecticide [Sevin 480 SC (2.25 mL per L)].The bottom of the bottles was removed and an acrylic vial (12.0 × 12.0 × 3.8 cm) containing water and detergent was attached bellow to collect the dead insects.The treatments were 1,4dimethoxybenzene (one or two dispensers per trap), 1,4-dimethoxybenze + indole, 1,4-dimethoxybenzene + trans-cinnameldehyde and control (n = 10).Traps were placed in the field on February 10, 2003.The population levels of A. viariegatus allowed the assessment of responses to volatile attractants.The volatile average release rate (over ten days) per dispenser was approximately 32 mg day -1 under laboratory conditions (Arruda-Gatti et al., 2006).
The experiment was conducted in a randomized complete block design.The distance between traps was 5 m within a block, and 10 m between blocks.An analysis of variance (ANOVA) was performed.Individual means were compared using Duncan's multiple range test (SAS Institute, 1989).

RESULTS AND DISCUSSION
The 1,4-dimethoxybenzene lured traps caught more beetles than the control in the first and second assessments (Table 1).Ten days after the onset of the experiment, there were no differences in the number of beetles caught by treatments probably due to the complete volatilization of the lure.Double dosages of 1,4-dimethoxybenzene did not improve beetle captures.Captures were higher in the 1,4dimethoxybenzene + cinnamaldehyde treatment than in 1,4-dimethoxybenzene only in the first assessment.Adding indole to 1,4-dimethoxybenzene did not improve beetle captures.
1,4-dimethoxybenzene is a major volatile floral chemical in Cucurbita maxima Duchesne cv.True Hubbard and the 4 th major component in Blue Hubbard floral volatile (Andersen, 1987).The neotropical D. speciosa is attracted by traps lured with 1,4dimethoxybenzene (Ventura et al., 2000).However, no records of attraction of North American Diabrotica inhabitants by 1,4-dimthoxybenzene have been reported although C. maxima blossoms do attract them (Metcalf