A biological fungicide product containing B. subtilis cells (60 g) and metabolites (60g) was transformed into a wettable powder formulated with, clay (480 g), surfactant (7,92 g) and water (2400 ml).The product showed to be similar to the conventional fungicides used in the control of Rhizoctonia solani, Aspergillus sp. and Sclerotinia sclerotiorum in bean seeds. The control of Pyricularia oryzae and Rhinchosporium sativum in rice seeds and Cercospora kikuchii, Phomopsis phaseoli and Fusarium spp. in soybean seeds was also as efficient as the comercial fungicides. For Dreschlera oryzae in rice seeds and Bipolaris sorokiniana, P. oryzae and Alternaria tenuis in wheat seeds, the treatment with the product, althought not as efficient as the chemical treatment, was statistically distinct from the control. The root nodulation of bean and soybean by nitrogen fixing bacteria, was not affected when the product was simultaneously applied with bacteria ( Rhyzobium and Bradyrhizobium japonicum). The product did not affect seedling emergence in any of the tested crops.
biological control; seed microbiolization; seed pathology; rice; bean; soybean; wheat