Open-access A mixed beta regression approach for analyzing bounded sensory data in incomplete block designs

ABSTRACT

Sensory analysis plays a crucial role in the food industry, facilitating innovation and enhancing products. This study includes a sample of individuals, both trained and untrained, who evaluate a product using a hedonic scale or scoring system within a balanced incomplete block (BIB) design. In this context, integrating sensory analysis with robust statistical methods that account for the nature of the response variables is essential to the success of the experimental study. Techniques for analyzing sensory data include response surface models and categorical models. This article proposes using beta regression as a viable alternative to the proportional odds model to address convergence issues, particularly given the number of parameters involved. Furthermore, the beta distribution offers flexibility for modeling data with heteroscedasticity and skewness. To support this approach, we conducted simulation studies that demonstrated agreement rates in product selection using both models. Additionally, we present a compelling case study to guide the selection of grape juice formulations. In this application, the findings from the mixed beta regression model reinforced the choices made using the proportional odds mixed model.

Keywords:
likelihood procedure; simulation studies; formulation selection; sensory attributes; random effects

Introduction

Sensory analysis is essential for assessing the acceptability of food products, covering evaluations from raw materials to final formulations. This analysis examines various attributes, including color, flavor, acidity, and aroma, through the senses of sight, touch, smell, and taste (Duan et al., 2024; Kottaridi et al., 2023). Nevertheless, challenges such as panelist fatigue and high costs can arise. These issues can be addressed by employing incomplete block designs, which enhance efficiency and accuracy (Sipos et al., 2021).

In this scenario, hedonic scales are commonly employed in sensory evaluation, producing ordinal data that may be influenced by panelists’ memory biases, potentially leading to variability and errors (Sugumar and Guha, 2022). It is crucial to utilize appropriate statistical methods to reduce these sources of error and enhance the reliability of the conclusions drawn from the data. Several studies in the literature treat these scores as continuous variables and apply analysis of variance, linear regression, or multivariate techniques, assuming that the underlying assumptions are robust (Smithson and Verkuilen, 2006). However, this approach is often incorrect and can result in misleading conclusions. While nonparametric tests, such as Friedman's test, are frequently used, they tend to be less powerful than parametric tests. Conversely, the generalized logit model offers an efficient alternative, although it may encounter challenges such as convergence issues and the necessity for category grouping (Agresti, 2010).

To address these limitations, this study explores the use of beta regression models with random effects for analyzing sensory data. Given their inherent flexibility, beta regression models are well-suited to handling skewed and heteroscedastic data constrained to bounded intervals (Kubinec, 2023). Therefore, these models bypass the assumptions of normality and homogeneity of variance (Junaid et al., 2024), thereby minimizing complications associated with convergence, overparameterization, and the necessity for grouping in logit models. Additionally, we present a simulation study that compares the performance of cumulative logit models and beta regression, followed by an application to real sensory data.

Materials and Methods

The dataset used in this study was obtained from a sensory evaluation conducted in Piracicaba, São Paulo State, Brazil (22°42’30" S, 47°37’30" W, altitude 546 m) in 2025. The experimental design employed a balanced incomplete block (BIB) design, where each of the 98 untrained panelists assessed four of the eight grape juice formulations. The tested products included eight commercial grape juice brands, divided equally into two categories: four whole juices (100 % juice or not from concentrate; formulations F873, F661, F419, and F571) and four reconstituted juices (from concentrate; formulations F715, F179, F732, and F318).

Each panelist rated the four samples on a five-point hedonic scale, with higher scores indicating more favorable perceptions. The evaluated sensory attributes included acidity, aroma, color, sweetness, and flavor.

Review of the beta regression model with a random effect

The beta regression model is well-suited for response variables that are strictly bounded within the open interval (0, 1), such as rates and proportions. Beta regression models that incorporate random effects can be viewed as extensions of generalized linear mixed models (GLMMs) (Figueroa-Zúñiga et al., 2013). In GLMMs, the linear predictor integrates fixed and random effects to address correlation structures that typically arise from repeated measurements within subjects (Salinas-Ruíz et al., 2023).

Consider a longitudinal study where yi=(yi1,,yini)T is a vector of ni observations for the i-th individual, with yij denoting the j-th response and i = 1, 2, … N. Let xij= (xij1, …, xijp)T be the (ni × p) matrix associated with the vector β = (β1, …, βp)T of unknown regression coefficients related to fixed effects, and zij = (zij1, …, zijq)T be the (ni × q) matrix related to the random effects vector uij = (ui1, …, uiq)T. Assuming Yij | ui are conditionally independent random variables and follow a beta distribution (μij, ϕ); u1, …, uq independent random variables with distribution uiσu2Nq(0,σu2I), the mixed beta regression model is defined as:

(1) g ( E ( Y i u i ) ) = x i T β + Z i T u i = η i

where g(.) is a known, strictly monotonic, and twice differentiable link function. The logit link function is commonly used (though not mandatory) and transforms Eq. (1) into (Zimprich, 2010):

(2) log ( μ i j / 1 μ i j ) = η i j = x i j T β + Z i j T u i

Accordingly, the conditional mean μij= E (Yij | ui) by means Eq. (2) is expressed as:

μ i j = exp ( η i j ) / ( 1 + exp ( η i j ) )

For further methodological details on beta regression models and their extensions, refer to Verkuilen and Smithson (2016), Stroup (2012), Zimprich (2010), Huang and Oosterlee (2011), and Pereira et al. (2013).

Proposed method

In sensory studies structured under a BIB design, v treatments (or formulations) are evaluated across b blocks (panelists), with each panelist assessing k treatments. Each treatment appears in exactly r blocks, and every pair of treatments co-occurs in precisely ƛ blocks. Let L sensory attributes be evaluated using a hedonic scale with c response levels. The BIB design must satisfy the following conditions (Yu et al., 2024): bk = rv.

Number of variety pairs:

( v ( v 1 ) / 2 )

Number of treatment pairs within each block:

( k ( k 1 ) / 2 )

Number across the entire experience:

( b k ( k 1 ) / 2 ) = ( λ v ( v 1 ) / 2 )

In the last equality, replacing bk with rv, we have:

λ ( v 1 ) = r ( k 1 )

These relationships facilitate balanced comparisons across treatments and pairwise comparisons. To assess the adequacy and sensitivity of this design under the specified conditions, a simulation-based power analysis was conducted using the SIMR package in R software (version 4.3.2) (Green and MacLeod, 2016). The simulation replicated the experimental setup in which 98 untrained panelists evaluated four out of eight formulations. Sensory scores were simulated under the assumption of a fixed difference of 0.15 between formulations F873 and F715, while incorporating random variation among panelists. The response variable was constrained to the interval (0, 1) to reflect the bounded nature of hedonic scores.

A mixed-effects model was fitted using the lmer() function from the lme4 package (Bates et al., 2015), with formulation as a fixed effect and panelist as a random intercept. Power analysis based on 100 Monte Carlo simulations yielded a power estimate of 99 % (95 % confidence interval: 94.6-99.97 %) for detecting the specified effect size with the current sample size of 392 observations. The large number of observations, combined with the balanced BIB design, provided sufficient degrees of freedom, confirming that the design possesses adequate sensitivity to identify meaningful differences between formulations (Morris et al., 2006).

To apply the modeling framework discussed in the section "Review of beta regression model with random effect", the original responses yi, which were collected on a five-point hedonic scale (ranging from 1 to 5), were rescaled by dividing each score by five. To ensure the values fell strictly within the open interval (0, 1), as required by the beta distribution, we applied the transformation proposed by Smithson and Verkuilen (2006):

(3) y i = ( y i ( n 1 ) + 0.5 ) / n

where n = bk. After this transformation, the scores are treated as realizations of a continuous variable with bounded support (Sarzo et al., 2023), in accordance with the beta regression class proposed by Ferrari and Cribari-Neto (2004).

To evaluate the product's influence on a sensory attribute, three models were considered. The null model is:

(4) η 0 = log ( μ i / 1 μ i ) = η 0

which assumes that the mean score of the sensory attribute is independent of the formulation. The second model includes the formulation effect:

(5) η 1 = log ( μ i / 1 μ i ) = α 0 + X i T β

where xi=(xit1,,xitk)T with 1 ≤ t1, …, tkv, representing the formulation indices in each block (panelist), and β = (β1,.., βv)T is the associated coefficient vector capturing the effect of formulation. In this setup, the dimensions are: the design matrix x (bk × v), the response vector y (bk × 1), and the coefficient vector β (v × 1). The design matrix includes dummy variables for the formulations. The third model includes random effects to account for the incomplete block structure and the uncontrolled panelist variability:

(6) η 2 = log ( μ i / 1 μ i ) = α 0 + X i T β + u i

where ui~N(0,σu2I) corresponding to a specific case of the general Eq. (2) for a given sensory attribute L. For all models (Eq. 4, 5, and 6), the conditional mean is obtained by:

(7) μ ^ i = exp ( η i ) / 1 + exp ( η i )

Parameter estimation for the mixed beta regression model (Eq. 6) was performed using maximum-likelihood estimation. The likelihood contribution from panelist i is given by:

(8) f i ( y i β , σ u 2 , ϕ ) = j = 1 k f i j ( y i j u i , β , ϕ ) f ( u i σ u 2 ) d u i

where fij(yij | ui, β, ϕ) is the conditional beta density for the observation yij (i.e., the j-th evaluation in block i), and f(ui|σu2) is the density of the random effect ui~N(0,σu2I). The overall likelihood function is:

(9) L ( β , σ u 2 , ϕ ) = i = 1 b f i ( y i β , σ u 2 , ϕ ) = i = 1 b j = 1 k f i j ( y i j u i , β , ϕ ) f ( u i σ u 2 ) d u i

Maximizing Eq. (9) yields estimates of the formulation effects (β), panelist variance (σu2), and the precision parameter (f). Taking the natural logarithm of Eq. (9) yields the log-likelihood function:

(10) ( β , σ u 2 , ϕ ) = i = 1 b log j = 1 k f i j ( y i j u i , β , ϕ ) f ( u i σ u 2 ) d u i

The primary computational challenge lies in evaluating the b integrals over the q-dimensional random effects ui, which lack a closed-form solution. To address this issue, the integrals were approximated using the Laplace method. This approach approximates the integral as a weighted sum centered on the integrand's mode. The method expands the integrand around the mode of the random effects through a second-order Taylor series and approximates the integral by computing a Gaussian integral at that mode. It is particularly effective for models with non-Gaussian responses and latent variables (Tierney and Kadane, 1986).

The model was estimated using R software with the glmmTMB package, which is built on the Template Model Builder (TMB) framework. This framework allows for the specification of complex likelihood functions that incorporate random effects and facilitates optimization through the Laplace approximation. The code is available at: https://github.com/GabrielRPalma/MixedBetaRegression/tree/main.

After model fitting, selection was based on the maximized log-likelihood, Akaike Information Criterion (AIC), and the Likelihood Ratio Test (LRT). All comparisons were evaluated at the 5 % significance level. The LRT statistic is defined as:

Λ = 2 [ log ( θ ^ 0 ) log ( θ ^ 1 ) ]

where log(θ^0) and log(θ^1) are the maximized log-likelihoods under the reduced and full models, respectively. Under standard conditions, Λ~χdf2, where df is the difference in the number of parameters between the two models. The AIC was computed as:

AIC = 2 log ( θ ^ ) + 2 k

where log(θ^) is the maximized log-likelihood, and k is the number of estimated parameters in the model.

Simulation studies

A simulation study was conducted to evaluate the performance of the beta regression model with random effects in sensory data analysis. This simulation utilized three formulations, arranged in a balanced complete block design. The objective was not to explore alternative experimental designs but rather to assess the modeling strategy compared with the commonly employed cumulative logit model with proportional odds.

Data were generated using parameters from a fitted mixed cumulative logit model with proportional odds, as defined below:

η i = log [ γ i / ( 1 γ i ) ] = α i + β T x + u i

where αj is the intercept for the j-th response category corresponding to a specific sensory attribute (such as acidity, color, aroma, sweetness, and flavor), β is the vector of fixed effect coefficients associated with the design xi, and ui~N(0,σu2I) represents the random effect for the i-th panelists. The parameter values used in the simulations are presented in Table 1. The reference category for the response variable was "1 = dislike extremely", and formulation F1 served as the reference level.

Table 1
Fixed parameters of the proportional probability model, used to simulate data in sensory analysis considering 13 scenarios.

After generating the data, both the cumulative logit models with proportional odds and the beta regression model with random effects were fitted. The proportional odds logit models (Eq. 11) were estimated using the clmm2function from the ordinalpackage (Christensen, 2015), whereas the beta regression model with random effects (Eq. 6) was implemented using the glmmTMBpackage (Brooks et al., 2017), as detailed in before.

For each scenario, 1,000 simulated datasets were generated for two sample sizes (N = 90 and N = 300 panelists). Agreement rates between the two fitted models are reported in Table 2, and the simulation scenarios are summarized in Table 1.

Table 2
Agreement rates between the cumulative logit model with proportional odds and the beta regression model with random effects for 13 scenarios in the simulation study, considering three formulations (F1, F2, and F3), for N = 90 and N = 300.

The results demonstrate that, for N = 90, the agreement rates between the models defined by Eq. (11) and (6) were at least 91 % across all scenarios. These rates showed a steady increase with N = 300, although a few exceptions were noted (e.g., F2 = F3 < F1, F1 = F2 = F3, and F3 = F1< F2), likely due to stochastic variation and random effects.

Certain configurations, specifically F1 < F3 < F2, yielded agreement rates as high as 99.9 % for N = 90 and 100 % for N = 300. This pattern was consistently observed across several scenarios, indicating strong agreement among the models regardless of sample size.

Overall, the simulation results show that the beta regression model with random effects aligns closely with the cumulative logit model, making it well-suited for analyzing sensory data collected on bounded ordinal scales.

Results

In this study, the original ordinal scale was converted to a continuous scale in the interval (0, 1) using Eq. (3), enabling the use of beta regression models. Additionally, an exploratory analysis was conducted to examine the distribution of responses across sensory attributes.

Boxplots illustrating the scaled evaluations (ranging from 0 to 1) for each sensory attribute are shown in Figure 1. Most formulations exhibited asymmetric response distributions across all attributes, except for F715 and F732 for acidity and flavor, and F715 for sweetness and F732 for aroma, which showed approximately symmetrical patterns. Outliers were detected in several formulations, notably for color (F179, F318, F419, F571), sweetness (F179), and aroma (F318 and F661).

Figure 1
Boxplots of the sensory attributes for the eight juice formulations, based on a sensory study conducted in Piracicaba, São Paulo State, Brazil, in 2025.

Median values were equal to or greater than 0.8 for most formulations. Exceptions included F715 and F732 for acidity; F715, F732, and F318 for flavor; F715 for sweetness; F318 and F732 for aroma, each with a median of approximately 0.6, and F732 for color, with a median of 0.4.

This exploratory analysis offers an initial overview of the data distribution. As outlined in the methodology, beta regression models were applied to all sensory attributes. Three model structures were evaluated: the null model (Eq. 4), which includes only the intercept; Model 1 (Eq. 5), which accounted for the fixed effect of formulation; and Model 2 (Eq. 6), which incorporates both formulation as a fixed effect and panelist as a random effect. To determine the most suitable model, we assessed the estimated dispersion parameter (ϕ^), the logarithm of the maximized likelihood function (log(L)), and the AIC. A summary of these metrics is provided in Table 3.

Table 3
Estimated dispersion parameter ϕ^, the logarithm of maximized likelihood function (log(L)) and Akaike Information Criterion (AIC) for all sensory attributes, considering the three models: Null (Eq. 4), formulation effect (Eq. 5) and with formulation and random effect (Eq. 6) referring to the study carried out in Piracicaba, São Paulo state, Brazil, in 2025.

The log-likelihood, estimated dispersion parameter, and goodness-of-fit statistics presented in Table 3 progressively increased from the null model to Model 2. Since these metrics indicate the quality of model fit, higher values signify a better fit. Incorporating a random effect for the panelist as an incomplete block proved relevant for elucidating response heterogeneity, as evidenced by lower AIC values for the mixed model across most attributes. Furthermore, the likelihood ratio test for the inclusion of the random effect (p < 0.01) confirmed its significance for all attributes except color, supporting the models described by Eq. (6) as the more suitable functional structure for the data. The estimated variances of the panelist random effect for each attribute were as follows:

σ ^ a c i d i t y 2 = 0.20 , σ ^ a r o m a 2 = 0.15 , σ ^ s w e e t n e s s 2 = 0.13 , σ ^ f l a v o r 2 = 0.12 , σ ^ c o l o r 2 = 0.06

The magnitude of the variance of the panelist random effects for nearly all attributes not only supports the structure of the chosen design but also demonstrates the mixed beta model's sensitivity in identifying variability in responses influenced by unmeasurable factors represented by these random effects.

For the "color" attribute, the model with only the fixed effect had a slightly lower AIC than the mixed model.

The marginal predicted means obtained from the fitted model are summarized in Table 4. Formulations F419, F179, and F571 achieved the highest overall sensory scores across various attributes. Notably, formulation F419 consistently recorded the highest predicted values for aroma (0.8375), flavor (0.7995), and sweetness (0.7833), suggesting greater consumer acceptance. In contrast, formulations F715 and F732 had the lowest estimated means across multiple attributes, particularly for aroma (0.6450 and 0.6451, respectively) and color (0.7808 and 0.5761, respectively). These findings align with the mixed cumulative logit models; however, for brevity, these details are not included in this study.

Table 4
Means estimated values and standard deviations (between parentheses) by Eq. (7) according to the sensory attributes and formulations, resulting from the study carried out in Piracicaba, São Paulo State, Brazil, in 2025.

Discussion

Most sensory tests used for product evaluation produce ordinal categorical data from hedonic scales. Cumulative logit models are the standard method for this type of data (Agresti, 2010), and their applications in sensory science are well-documented (Gadrich et al., 2022; Fatoretto et al., 2018). Despite their theoretical robustness, these models often suffer from overparameterization and convergence issues, limiting their applicability to complex experimental designs.

Alternatively, beta regression models with random effects are particularly well-suited to analyzing hedonic scores transformed to the interval (0, 1). Our proposal approach addresses data asymmetry and heteroscedasticity while incorporating evaluator-specific variability through random intercepts. Moreover, this modeling approach allows prioritizing of formulations with desirable profiles, even under ingredient constraints. In this context, the approach offers methodological and statistical advantages and benefits researchers by improving product selection.

Simulation results show that beta regression produces estimates that align with those generated by the cumulative logit model. However, it offers superior numerical stability, better convergence behavior, and greater interpretability of both parameters and results. These advantages underscore its potential as a reliable analytical framework for sensometric applications.

Applying the model to real data from a grape juice sensory study demonstrated its effectiveness in capturing both formulation effects and interindividual variation. Formulations with higher juice content (F419 and F571) received the highest hedonic scores. However, F179, despite its lower juice concentration, also received favorable evaluations. These findings suggest that certain sensory attributes, such as flavor balance and sweetness, can offset compositional shortcomings. This aligns with the observations of Rossiter et al. (2000) for Actinidia deliciosa (A. Chev.) C.F. Liang & A.R. Ferguson, in which higher °Brix levels reduced perceived acidity and enhanced consumer preference.

From an applied perspective, these findings underscore that achieving high sensory acceptance can be attained not only through compositional enrichment but also through targeted sensory optimization. The proposed modeling approach enables prioritizing formulations with desirable profiles, even under ingredient constraints. Additionally, its integration with BIB designs has proven advantageous in scenarios where panelists have limited evaluation capacity. BIB designs yield conclusions comparable to those from complete block designs while reducing experimental time by more than 40 % and costs in consumer research (Silva et al., 2014).

Despite its strength, certain limitations persist. The current model assumes that sensory attributes are independent, a premise that may not always hold across contexts. Furthermore, while the logit link is commonly used in beta models, exploring alternative link functions such as probit, complementary log-log, or Cauchy could yield a better fit, depending on the data distribution, and warrants further investigation.

Another limitation is the lack of formal residual diagnostics. Although the model has shown strong performance in both simulations and empirical applications, diagnostic tools are crucial for verifying model assumptions and identifying outliers (Espinheira et al., 2017). There remains a scarcity of specific techniques for beta models with random effects; for instance, the quantile residuals proposed by Pereira (2019) are limited to models without random effects. To enhance diagnostic assessment, it is advisable to utilize tools such as the quantile residuals from the DHARMa package (Hartig, 2024) or half-normal plots with simulated envelopes (Moral et al., 2017).

Future research should explore multivariate modeling techniques, such as Dirichlet regression and Bayesian hierarchical models, to address attribute correlations and improve predictive accuracy. Additionally, there is a need for studies focusing on residual analysis and diagnostics, particularly in the context of random effects. A promising avenue for expanding the application of this approach in applied sensory science is the simulation-based optimization of BIB designs, which can be explored across various panel sizes, treatment structures, and formulation subsets. Furthermore, a unified analysis of multiple sensory attributes could help optimize outcomes and improve interpretability.

  • Declaration of use of AI technologies
    The authors declare that they did not use artificial intelligence technologies for text generation, data analysis, or any other stage of the development of this manuscript. All content was produced independently by the authors.

Data availability statement

The data in this study contains sensitive and confidential information, protected by legal regulations; therefore, it cannot be shared publicly.

Acknowledgments

This publication has emanated from research conducted with the financial support of Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) process number (88887.821274/2023-00) and Science Foundation Ireland under Grant number 18/CRT/6049. Special thanks to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), process number 300155/2025-5

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Publication Dates

  • Publication in this collection
    14 Aug 2026
  • Date of issue
    2026

History

  • Received
    02 Apr 2025
  • Accepted
    07 Oct 2025
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