# Abstracts

Nutrition and fertilization are important factors in determining fruit yield and fruit quality. There are several methods for plant nutritional status diagnosis, among them, two are relevant and named as Sufficiency Range Approach (SRA) and Diagnosis and Recommendation Integrated System (DRIS). This research reports the main concepts and applications of DRIS in nutritional diagnosis of fruit crops, comparing it with current nutritional diagnosis methods, indicating advantages and disadvantages, and possible limitations to be investigated.

foliar analysis; fruit crops; mineral nutrition

A nutrição e a adubação são fatores determinantes na produtividade dos pomares e na qualidade de frutos. Dentre os diversos métodos de diagnose nutricional das plantas, destacam-se o critério de faixas de suficiência (CFS) e o sistema integrado de diagnose e recomendação (DRIS "Diagnosis and Recommendation Integrated System"). São relatados neste trabalho os principais conceitos e aplicações do método DRIS na diagnose nutricional em fruteiras, comparando-o com os sistemas atuais de diagnose nutricional, apontando vantagens e desvantagens, e possíveis limitações a serem investigadas.

análise foliar; fruticultura; nutrição mineral

REVIEW

DRIS: concepts and applications on nutritional diagnosis in fruit crops

DRIS: conceitos e aplicações na diagnose nutricional em plantas frutíferas

Francisco de Assis Alves Mourão Filho* * Corresponding author < famourao@esalq.usp.br>

USP/ESALQ - Depto. de Produção Vegetal, C.P. 9 - 13418-900 - Piracicaba, SP - Brasil

ABSTRACT

Nutrition and fertilization are important factors in determining fruit yield and fruit quality. There are several methods for plant nutritional status diagnosis, among them, two are relevant and named as Sufficiency Range Approach (SRA) and Diagnosis and Recommendation Integrated System (DRIS). This research reports the main concepts and applications of DRIS in nutritional diagnosis of fruit crops, comparing it with current nutritional diagnosis methods, indicating advantages and disadvantages, and possible limitations to be investigated.

Key words: foliar analysis, fruit crops, mineral nutrition

RESUMO

A nutrição e a adubação são fatores determinantes na produtividade dos pomares e na qualidade de frutos. Dentre os diversos métodos de diagnose nutricional das plantas, destacam-se o critério de faixas de suficiência (CFS) e o sistema integrado de diagnose e recomendação (DRIS "Diagnosis and Recommendation Integrated System"). São relatados neste trabalho os principais conceitos e aplicações do método DRIS na diagnose nutricional em fruteiras, comparando-o com os sistemas atuais de diagnose nutricional, apontando vantagens e desvantagens, e possíveis limitações a serem investigadas.

Palavras-chave: análise foliar, fruticultura, nutrição mineral

INTRODUCTION

One of the main plant mineral nutrition objectives is increasing net incomes through efficient fertilization management. To attain this goal, it is initially necessary to correctly determine the yield-limiting impact of a given nutrient.

The search for an effective method to determine plant nutritional status has been the target of many researches in plant nutrition. Current methods include both soil and tissues analysis. The advantage of this latter was already observed in early studies of Chapman & Brown (1950). The soil analysis method is based on the assumption that the chemical extractants simulate the root system acquisition of soil nutrients in a comparable manner. However, it does not take into account factors such as soil temperature and aeration, and even the higher or lower absorption due to the own plant nutritional needs. Another soil analysis limitation is soil sampling, which is supposed to actually represent the soil portion explored by the roots (Reuther & Smith, 1954).

Tissue analysis is considered a more direct method of plant nutritional status evaluation than soil analysis, but that method must necessarily involve a well-defined plant part analysis (Hallmark & Beverly, 1991). Among the several tissues to be considered for nutritional diagnosis purposes, leaves constitute the main plant sampling material (Chapman & Brown, 1950). The improvement in tissue analysis techniques enabled the comparison of results from different soil type fertilization experiments. In the same way, it allowed the evaluation of fruit crop plants response to treatments in nutrient solution experiments (Reuther et al., 1958).

Leaf analysis can be a very useful tool for plant nutritional diagnosis, since adequate procedures are available for data analysis. Because of the dynamic nature of the leaf tissue composition, strongly influenced by leaf age, maturation stage, and the interactions involving nutrient absorption and translocation, the tissue diagnosis may be a practice of difficult understanding and utilization (Walworth & Sumner, 1987). Several methods for nutritional diagnosis using leaf tissue analysis have been proposed and used, including the critical value (CV), the sufficiency range approach (SRA), and the diagnosis and recommendation integrated system (DRIS). Considering that DRIS uses the nutritional balancing concept (relationship among nutrients), it is postulated that this method might be more precise than the others in the detection of nutritional deficiencies or/and excesses.

2- The DRIS method

The usual methods for leaf chemical analyses interpretation presuppose the nutrient concentration comparison with reference values (critical concentrations or sufficiency ranges). Nutrient concentrations far below or above reference values are associated with decreasing vegetative growth, yield and quality. These methods intend to evaluate isolated deficiency or excess values, without measuring the overall nutritional balance. Moreover, researches related to this subject indicate a great difficulty in establishing consistent critical values and relate them with high yields, mainly because the nutritional status varies with leaf tissue maturation. Thus, sampling definition is a fundamental step for better accuracy of these methods.

A new interpretation for leaf analysis was firstly developed and proposed by Beaufils (1957; 1971; 1973) for rubber trees (Hevea brasiliensis), named as diagnosis and recommendation integrated system (DRIS). The DRIS method uses nutrient ratios instead of absolute and/or individual nutrient concentrations for interpretation of tissue analysis. Soon after the initial proposed DRIS norms for leaf analysis interpretation were released, further ones were developed for other agricultural, forest and horticultural crops. In countries such as United States, Canada, and China, DRIS is being adopted as part of a representative diagnosis in selected areas (Lopes, 1998; Hallmark & Beverly, 1991; Walworth & Sumner, 1987).

2.1- DRIS method theoretical basis

The DRIS method expresses results of plant nutritional diagnosis through indices, which represent, in a continuous numeric scale, the effect of each nutrient in the nutritional balance of the plant. These indices are expressed by positive or negative values, which indicate that the referred nutrient is in an excess or deficiency, respectively. The closer to zero are the indices for all the nutrients, the closer will be the plant to the adequate nutritional balance (Beverly, 1991; Walworth & Sumner, 1987).

The working premises for DRIS are based on: (a) the ratios among nutrients are frequently better indicators of nutrient deficiencies than isolated concentrations values; (b) some nutrient ratios are more important or significant than others; (c) maximum yields are only reached when important nutrient ratios are near the ideal or optimum values, which are obtained from high yielding-selected populations; (d) as a consequence of the stated in (c), the variance of an important nutrient ratio is smaller in a high yielding (reference population) than in a low yielding population, and to the relations between variances of high and low yielding populations can be used in the selection of significant nutrient ratios; (e) the DRIS indices can be calculated individually, for each nutrient, using the average nutrient ratio deviation obtained from the comparison with the optimum value of a given nutrient ratio, hence, as pointed by Jones (1981) and Walworth & Sumner (1987), the ideal value of the DRIS index for each nutrient should be zero.

In general, the DRIS has some advantages over other diagnosis methods: presents continuous scale and easy interpretation; allows nutrient classification (from the most deficient up to the most excessive); can detect cases of yield limiting due to nutrient unbalance, even when none of the nutrients is below the critical level; and finally, allows to diagnose the total plant nutritional balance, through an unbalance index (Baldock & Schulte, 1996). An additional advantage of DRIS, acknowledged by some authors but rebuted by others, is that, overall, it is less sensitive to tissue aging in comparison to others (Walworth & Sumner, 1987). Tissue aging influence the nutrient concentration (nutrient content/ dry matter); several examples are reported in the literature, including studies in alfalfa, potato, corn, peach, and many other agricultural and horticultural crop species. Although some exceptions may occur, concentrations of nitrogen, phosphorus, potassium and sulfur tend to decrease with tissue aging. On the other hand, calcium and magnesium concentrations tend to increase in older tissues (low mobility), in spite of the opposite being reported in the very early or later stages for some crops. The dynamic nature of the plant tissue mineral composition tends to restrict the use of leaf analysis for nutritional diagnosis. As already stated, the criteria of critical levels or sufficiency ranges generally depend on norms for diagnosis derived from an specific plant tissue part and age, and classifies the plants based solely in the leaf nutrient concentration (leaf nutrient content/ leaf dry matter). Thus, the plant growth stage for leaf sampling is an essential factor for the application of both methods, and therefore, the diagnoses based on these criteria are usually applied in leaf samples obtained from a well-defined growth stage.

An important limitation of these methods is that, especially in some annual crops, the established standard sampling period many times occurs too late in the growing season, so that fertilizer application will not be effective to correct a nutritional problem, or may not match the sudden symptoms of a nutritional disorder, when the producer mostly need the information (Walworht & Sumner, 1987). To overcome this problem, it would be necessary to get nutritional reference values for several maturation stages and, as a matter of fact, some of these standards have already been established for a few crops. Although simple in theory, this procedure is of difficult application. First, there is a need for precise definition, at the sampling time, of plant maturation stages (or growth period) in the field. Later, the sampler should communicate this information to the analyst or person taking care of the diagnosis, so that appropriate norms can be selected and used. In addition to these limitations, little research has been developed to determine the influence of the cultivar in the nutrient concentration in a given maturation or development stage. Finally, factors that affect the tissue aging rate might also influence the relation between nutrient concentration and maturation.

An option for these diagnosis methods was proposed through the DRIS (Beaufils, 1973), which defined that, in general, nitrogen, phosphorus and potassium concentrations decrease with tissue maturation. Therefore, the ratios N/P, N/K, and P/K (or reciprocal ratios) should be kept constant. In the same way, because of concentrations of Ca and Mg generally increase with maturation, quotients between these nutrients (Ca/Mg or Mg/Ca) should result in constant values. Moreover, the product of two nutrients, with concentrations running in opposite directions with time (N ´ Ca, for example), also should remain constant.

An example of nutrient ratio use is illustrated in Table 1, where nutrient concentrations in corn tissues are expressed in a dry matter basis or as nutrient ratios (Walworth & Sumner, 1987). The expression in a dry matter basis (%N, %P and %K) showed much more coherent correlation with tissue maturation (higher determination coefficients - r2) than the expression as nutrient ratios.

Such constancy in the nutrient ratios and their products cannot be applied in all cases. Several nutrient concentration rate can change very fast in young plants, and the nutrient ratios and their products can also vary in these cases. Even so, these expression forms are less affected by the maturation processes, therefore presenting great potential to expand usefulness and exactness of the leaf tissue diagnosis.

Nevertheless, DRIS advantages have already been contested, because for some crops, it showed to be as sensitive as SRA to plant tissue maturation and plant age (Baldock & Schulte, 1996). Moreover, additional limitations to the method can be pointed, such as the need for extensive and advanced computational calculations and equipment, results expressed in non-independent indices, and frequent occurrence of false diagnosis for some nutrient excesses.

Due to recent developments in both hardware and software resources, the difficulty in running the method turned to be of little importance. The non-independent indices are perhaps an advantage, because this might be the greater DRIS contribution in relation to the SRA. Other ten mistakes in the diagnosis through DRIS have also been identified, but many of them do not affects the method effectiveness in a relevant way (Hallmark & Beverly, 1991).

2.1.1 - DRIS norms

The first step for the implementation of any nutritional diagnosis method is the establishment of standards or norms, and the same applies for the DRIS method. The DRIS norms are always obtained in a high-yielding population, named reference population, which is selected from a larger population. The databases for definition of norms might have variable size in function of premises to be adopted in the method and should be uniform, regarding the crop characteristics. Norms obtained from a large database derived from different soil types, climates and cultivars, usually cannot be generalized, and they will be considered representative just if they include all the population variability. Therefore, these attributes should be previously well defined, and thus, be gathered to form the database (Letzsch & Sumner, 1984).

The database size might not be directly related to standard quality. DRIS norms developed from 10 cornfield observations, with yields exceeding 18 t ha-1, were more representative and efficient than norms deriving from larger databases (Walworth et al., 1988). Scientific literature reports a large variation in the database size for DRIS norms definition, from just 24 observations (Leite, 1992) up to about 2,800 (Sumner, 1977) or even more. However, although the latter presents a high quality, it is also more embracing because, it refers to a population of all corn cultivars and to the whole Southern Africa territory, while the first is originated from Conilon coffee plants, cultivated only in the north of the Espírito Santo State, Brazil. Maybe, too much generic DRIS norms can negatively affect the diagnosis efficiency. Despite the data quantity, the observation quality should be the goal for database choice.

The chosen population or database for norms definition should be subdivided in two sub-populations or categories (Beaufils, 1973; Beverly, 1991; Walworth & Sumner, 1987). These sub-populations are the following: a) Non-abnormal plants, or reference population, that are not influenced by adverse conditions and present yield significantly higher than an arbitrarily established level; b) Abnormal plants, or non-reference population, influenced by other factors, with lower yields than the established.

Several researches have revealed that the selection of the reference population is an important factor for the DRIS effectiveness and success. Walworth & Sumner (1987) alleged that the reference limit to separate two sub-populations should be arbitrarily chosen, because each sub-population ought to present normal distribution. Other authors recommended that the reference population contain, at least, 10% of the overall database observations (Letzsch & Sumner, 1984). Malavolta & Malavolta (1989) recommended the reference population to be obtained with 80% maximum yield observations.

DRIS norms are originated after the reference population definition, in other words, the relation between all the nutrients pairs and their respective standard deviations or coefficient of variation are obtained. The ratio between a pair of nutrients can be direct or inverse. The concentrations of nitrogen and phosphorus, for instance, can be related either as N/P or P/N ratio.

In DRIS calculi, each pair of nutrients is discriminated by only one expression. There are several criteria to select the best adequate expression and the most used is the variance largest ratio among high and low yielding populations (Letzsch, 1985; Walworth & Sumner, 1987). That same criterion was named "F value" (Nick, 1998). Bataglia & Santos (1990) evaluated direct and indirect ratios, concluding that the ratio order can interfere in the indexes results in citrus, especially if the function is obtained according to Jones (1981) proposal. Nick (1998) suggested the criterion named "r value" for the nutrient ratio order choice for DRIS application in pruned coffee plants, which is referred to the correlation coefficient calculation (r) between plant variable response values and the ratio between nutrient pairs, both in direct or inverse order. This ratio order that will result in higher correlation coefficient absolute value is the chosen one. Studies in citrus showed that the "r value" is an adequate criterion for the determination of the nutrient ratio order (Mourão Filho et al., 2002).

The DRIS for plant nutritional diagnosis can basically be applied in two forms: DRIS graphs and DRIS indices. The DRIS graphs are applied in the norms for only three nutrients and their ratios (Walworth & Sumner, 1987). Although the use of diagrams or graphs enable the diagnosis for three nutrients, DRIS still favors the mathematical ranking of the nutrient ratios or their products in nutritional indices, which can be easily interpreted. Initially, the DRIS reference or norms values should be determined, as already described, for all the nutrient ratios or products (for all nutrient pairs) to be used in the indices calculation.

After norms definition, sample analysis results are ready to be submitted to the DRIS indices calculation (Walworth & Sumner, 1987), which are composed of each nutrient individual index, calculated in two steps: first, the functions for each nutrient pair ratio, and second, the sum of functions involving each nutrient. Hypothetical A to N nutrient indices can, therefore, be calculated as follows (Walworth & Sumner, 1987):

where: When A/B is larger or equal to a/b,

Or, when A/B is smaller than a/b,

In these equations, A/B is the tissue nutrient ratio of the plant to be diagnosed; a/b is the optimum value or norm for that given ratio; CV is the coefficient of variation associated with the norm; and z is the number of functions in the nutrient index composition. Values for other functions, such as f(A/C) and f(A/D) are calculated in the same way, using appropriate norms and CV. In other words, one nutrient index is the average function of all the ratios containing a given nutrient. The components of this average value are pondered by the CV reciprocal of the high yielding populations (reference populations). Thus, if the A/B and A/C ratios are both used to generate an index for the A nutrient, the contribution of each one to the calculation of this index will be function of the CV values (reference ratios) associated to them, what will reflect the relative influence of these two expressions in the crop yield.

Several model modifications have been proposed to increase accuracy in the nutritional diagnosis for several crops. The calculation of the nutrient ratio functions is made according to one of three methods, namely: (a) the original method proposed by Beaufils (1973); (b) the Jones (1981) method; and (c) the Beaufils (1973) method, modified by Elwali & Gascho (1984). Although these nutrient function ratio calculation methods have been evaluated in some researches, there is not yet a clear definition for the best recommendation. The three methods applied to rubber trees revealed that Beaufils (1973) and Elwali & Gascho (1984) procedures presented similar results, and that Jones (1981) procedure showed dependence on the nutrient ratio (Bataglia & Santos, 1990).

In some citrus databases, the Beaufils (1973) method highlighted nutritional deficiencies, the Jones (1981) method had advantage for presenting more simple calculation and larger statistical formality, and the Elwali & Gascho (1984) method showed lesser interpretation errors (Santos, 1997).

According to Beverly (1991), there are two ways for the second and last stage of DRIS indices calculation (the function sum involving each nutrient) namely: DRIS (Beaufils, 1973) and M-DRIS (Hallmark et al., 1987; Walworth et al., 1986). The original DRIS method just uses the nutrient ratio functions. On the other hand, the M-DRIS method, a variation and expansion of original DRIS, foresees dry matter inclusion in the indices calculation. The expressions are identical to the ordinarily used, however, in this case, the dry matter is treated as an additional constituent and a new index is calculated, in the same way as for the other plant constituents. In fact, dry matter is, essentially, the sum of the concentration of three nutrients usually ignored in nutritional considerations: C, H, and O. That additional index is the dry matter mass index, a good indicator of the sampled tissue maturity regarding the standard.

The DRIS utilization can be enlarged for evaluation of other given data beyond the limits of those related to leaf analysis. Thus, norms for soil analysis tests were developed for P, K, Ca, and Mg to be applied in sugar cane crop in Southern Africa (Beaufils & Sumner, 1976). As in the leaf diagnosis, the use of DRIS for soil analyses results, presents the advantage of carrying considerations on the nutritional balancing and the nutrient ranking in terms of relative abundance to the optimum levels.

DRIS can also be expanded for expressions including non-essential elements, such as Si or Na, or even non-nutritional variables, like plant population or planting date, although such variables were not included in calibrations in already published diagnosis. Theoretically, nutrients such as nitrate and ammonium can be considered apart and treated as individual nutritional factors, in expressions within the method (Walworth & Sumner, 1987).

The absolute sum values of the nutrients indices generate an additional index denominated Nutritional Balance Index (NBI). This index can be useful to the plant nutritional status indication, without however, hinting their causes. The higher the sum value, the larger will be the indication of plant nutritional unbalance and, therefore, the lower will be the yield. NBI can be calculated for both DRIS and M-DRIS.

2.1.2- Interpretation of the DRIS nutritional indexes

The value of each ratio function is added to the subtotal of one index and subtracted from another [that is, the value f (A/B) is added to A index and subtracted from B index]; before the final ponderation, all the indexes are balanced around zero (Walworth & Sumner, 1987). Consequently, the sum of the nutritional indexes must be zero. When results are negative (lower than zero), that means deficiency, and the more negative the index, the higher the deficiency will be in relation to the other diagnosed nutrients. On the other hand, high index values (the more positive and distant from zero indexes) indicate excessive quantity of the considered nutrient relatively to the others.

The following example may illustrate the DRIS method interpretation, and to make it simple, this example refers only to nitrogen (N), phosphorus (P) and potassium (K). Other nutrients may be incorporated to the calculations using the same procedure. For a nutritional diagnosis in maize, the interpretation norms are presented in Table 2.

Considering a maize leaf sample with the following nutrient concentrations in the dry matter: N (3.30%), P (0.20%) and K (1.20 %), the calculations to be made are the ratios between the nutrients (represented in capital letters in the previous equations) that are: N/P = 3.30/0.20 = 13.5; N/K = 3.30/1.20 = 2.75; and N/P = 1.20/0.20 = 6.0.

Thus,

because N/P > n/p.

Applying the respective values, it will result: f(N/P) = [(16.5/10.04) 1] (1000/14) = 45.96.

In the same way,

The equation for the f(K/P) is, however, 1 [(k/p)/(K/P)] (1000/CV), because k/p > K/P and is equal to [1 6.74/6.00)] (1000/22) = -5.61. The other nutrient indexes are calculated:

N index = [f(N/P + f(N/K)]/2 = (45.96 + 40.27)/2 = 43

P index = [-f(N/P) f(K/P)]/2 = (-45.96 + 5.61)/2 = -20

K index = [-f(N/K) + f(K/P)]/2 = (-40.27 5.61) = -23

The N index (43) >> P index (-20) > K index (-23); thus, this result may be interpreted as: for a high yielding corn, the K is being relatively more required than P, which is more required than N.

A leaf sample with adequate nutritional balance will show all indexes equal to zero. However, it is possible to have a nutrient presenting an index equal to zero and not being at the adequate concentration. For example, supposing the following diagnosis results:

It might be concluded that N index would indicate the most deficient nutrient, compared to the others, and would probably be the most limiting nutrient if the yields were entirely related to the nutrition. And the P index equal to zero, would indicate a nutrient relatively less abundant than K and Ca or Mg and would be the second more deficient in this diagnosis. Nevertheless, in this case, because of nutrients may be added but not be removed from the soil, at least under ordinary conditions, the recommendations for this diagnosis would be addition of N, and addition of P in lower proportion, despite the P index equal to zero.

2.1.3 - Application of DRIS method to fruit crops

Since the original proposal, DRIS was developed for several horticultural, ornamental, and fruit species. Among the main horticultural species already diagnosed by this method are the lettuce (Sanchez et al., 1991), tomato (Caron & Parent, 1989; Caron et al., 1991; Hartz et al., 1998; Parent et al., 1993; Rouin et al., 1988; Mayfield et al., 2002), potato (Mac Kay et al., 1987; 1989; Meldal-Johnsen & Sumner, 1980; Navvabzdeh & Malakouti, 1993; Parent et al., 1994a), onion (Caldwell et al., 1994), cucumber (Mayfield et al., 2002), and carrot (Parent et al., 1994b)

There are few research works in the literature about the application of DRIS method in ornamental plants. Some of them refer to the Christmas pine (Abies fraseri) (Rathfon & Burger, 1991a; 1991b; Arnold et al., 1992).

There are reports on DRIS application to fruit crops and some DRIS norms were developed for application on cherries, Napolean cultivar, in the State of Oregon, USA (Davee et al., 1986). The DRIS indexes for each nutrient and the unbalance-index were calculated. Plants with high unbalanced indexes presented consistently low yield, but crop systems using mulching presented lower unbalanced indexes and relatively higher yields. These nutrient unbalanced indexes were more correlated with relative increases in yield than any other nutritional parameter. Some authors from the State of Oregon, using DRIS norms recommended for cherries and hazelnuts, have calculated the DRIS indexes for N, P, K, Ca, Mg, Mn, Fe, Cu, B and Zn in more than 1,000 leaf sample data for each crop (Righetti et al., 1988). The nutrient unbalanced indexes were obtained through the sum of values in module. The sample with the least nutrient unbalanced indexes was considered the ideal sample for their nutrient concentrations, and its values were used to create an artificial database and determining which critical values would be more consistent when compared to the DRIS evaluations. By means of calculations that maintained all but one nutrient in ideal levels, and varying another, the authors identified the concentration of each nutrient that would be associated to nutritional unbalance occurrence. Thus, besides the nutritional diagnoses evaluation based on the nutrient ratios, the norms for DRIS also allowed evaluating the SRA for nutrients that DRIS itself sometimes appoints as relatively deficient or excessive nutrients.

SRA and DRIS were also compared for hazelnuts, in the State of Oregon, USA (Alkoshab et al., 1988). The reference values, derived from both published and unpublished data, were used to calculate the DRIS indexes for N, P, K, Ca, Mg, Mn, Fe, Cu, B and Zn. The nutrient unbalanced indexes were also calculated. The SRA and DRIS diagnoses were compared to determine whether the relative nutrient deficiencies or excesses, associated to severely unbalanced plants, would be efficiently detected in leaf samples. From a total of 624 leaf analyses, the diagnoses resulted in agreement of both methods, especially when the sufficiency range was narrow for a given nutrient. On the other hand, some nutrients were not identified in the range of severely in excess or deficiency in any of the leaf samples classified as severely unbalanced, based on the DRIS indexes sum. N and Mg deficiencies were not detected, unless extremely low limits for the nutrient unbalance indexes were used. The use of such low limits for the N and Mg deficiencies unbalance indexes induced the identification of some high-yielding plants as nutritionally unbalanced. Thus, in this situation, DRIS was not effective in detecting nutrient deficiencies or toxicities and it was concluded that the method is a complementary tool to the SRA, providing additional information about nutritional unbalances.

The application of DRIS has also been considered for apple trees (Parent & Granger, 1989). DRIS norms were derived from an apple compact orchard in Canada. The experiment was installed in the Appalachian mountains region, at Quebec, with the Morspur McIntosh cultivar on the M.7, M.26, Ott. 3, and M. 9 dwarfing rootstocks. The plants received 12 different fertilization treatments involving commercial products based on N, P, K, Ca, and Mg in three rates. The higher yielding plants on the Ott.3 rootstock presented lower leaf Mg concentrations than on the other rootstocks. Variations in the norms, year by year, led to an annual norm definition. It was concluded that annual yields could be used instead of cumulative yields for the DRIS norms definition, especially after the sixth planting year. The incorporation of the dry matter index in the nutritional balance equations (M-DRIS) was important to better define the limiting and non-limiting nutrients, especially when the tissue samples were collected in a specific period, as is the case of fruit trees.

Research works carried out in Hungary investigated the DRIS standard ratios for apple orchards (Szucs et al., 1990). Data on yield and leaf nutrient concentration from 18 representative orchards were collected during three consecutive years. By means of conventional DRIS method calculations, the indexes indicated K-excess and P-deficiency, while the N concentrations were adequate. The norms estimated by quadratic regression analyses for N/P, N/K and K/P indicated K excess and relative N- and P-deficiency, suggesting that the norms obtained by regression analysis might possibly point out more extreme nutrient ratios than the traditional method.

DRIS norms and indexes involving N, P, K, Ca and Mg were established for apple orchards in New Zealand (Goh & Malakouti, 1992). DRIS was compared to the SRA and the conclusion was that both methods presented similar efficacy. Unbalances referred to the N-excess and Ca-deficiency were detected. The best sampling period for diagnosis purposes was 3 to 5 months after blooming.

Preliminary norms for DRIS in vineyards were determined in Germany (Schaller & Lohnertz, 1984). The indexes were calculated based on approximately 7,000 groups of leaf samples. The reference population (high productivity) was defined as that presenting high sugar content in the fruit must. The developed norms allowed the detection of limiting nutrient concentrations for productivity and quality, which could not be detected using the conventional methods. On the other hand, it was not possible to demonstrate coincident results between soil analysis and DRIS norms. By means of simple and multiple regression analyses, DRIS norms provided precise estimates for the next year yields.

In India, DRIS preliminary norms were derived from Thompson Seedless' grape, which indexes were evaluated in a low yielding vineyard (Chelvan et al., 1984). The DRIS norms were determined from a population in 48 plots, with three-year-old plants, cultivated with four N rates (300; 600; 900 and 1200 kg ha-1), four K2O rates (0; 500; 1000 and 1500 kg ha-1) in a factorial, and a unique P2O5 rate (500 kg ha-1). Plots that showed yields higher than 20 kg per vine plant (9 m2) were considered as high-yielding plots and those lower than 15 kg per vine plant in the same area, as low-yielding plots. Overall, for low productivity plots, high P- and low K-indexes were obtained. The most limiting nutrients were K and N in the plots that received only N in the rate of 900 kg ha-1. In another research in India, new criteria was developed to classify the N nutritional status of two grapevine cultivars based on the DRIS indexes calculated with soil and leaf analysis data (Bhargava & Raghupathi, 1995). Besides the new nutrient level adopted, new fertilization procedures were also recommended.

Research data on the nutritional diagnosis methods for pecan were also developed and preliminary DRIS norms were obtained from a data collection of more than 3,000 entries, including the yield and 11 nutrient concentrations. The reference population was selected from 25% best yielding plants (yield above 58 kg per plant) (Beverly & Worley, 1992).

Nutrient concentration ratios in peach leaves collected at different stages of maturation and plant yield of Batsch' cultivar were also determined (Sanz et al., 1992). Data were collected from 180 plants in several groves. Leaf samples from 60, 90, 120, 150 and 180 days after blooming were analyzed for nutrient concentrations and the respective yield was registered for each plant. Several calculations were performed, among them, the correlation between the nutritional data (nutrient concentrations and 10 nutrient ratios) and plant yield. The best correlation coefficients were obtained from leaf samples collected 60 and 120 days after blooming. Sanz (1999) performed a series of simulations to evaluate the DRIS method and some of its variations with the method DOP (Deviation from Optimum Percentage), using leaf samples of the Batsch' peach cultivar, and concluded that both methods presented similar efficiency.

The DRIS method was used to identify mineral deficiencies associated to the mango decline (a disorder of unknown etiology), in Tommy Atkins' cultivar orchards, in Florida, USA (Schaffer et al., 1988). The nutrient deficiencies associated to decline-affected plants were correlated to the whole orchard nutrition and not to the individual plant nutritional status. Higher nutrient unbalanced indexes were observed in highly affected than in healthy orchards. In the decline-affected plants, the nutrients considered deficient according to the DRIS method were Mn, Fe, or a combination of both. These nutrient concentrations were below the critical value in two of the three decline-affected mango orchards. The Mg concentration was higher, overall, in decline-affected plants than in healthy plants. In the affected orchards, phosphorus was the nutrient with the lowest DRIS index, but the average leaf P concentration was still above the critical value. It was concluded that DRIS used together with sufficient ranges, might be a good auxiliary tool to detect nutritional deficiencies in decline-affected mango orchards.

DRIS norms were developed for mango orchards, Alphonso cultivar, using a plant population from the Maharashtra district, India (Raghupathi & Bhargava, 1999). The reference population was defined within the productivity range of 5.4 and 7.4 t ha-1. Low yield was associated to low Mg concentrations. The same authors developed another similar research work with pomegranate (Punica granatum, L.) (Raghupathi & Bhargava, 1998).

Preliminary DRIS norms were also developed for N, P, and K for pineapple plantation, based on more than 1,100 observations (previously published) on the leaf nutrient composition and yields (Angeles et al., 1990). The data were separated in high yielding (above 60 t ha-1) and low yielding groves (below 60 t ha-1), and the DRIS norms were derived by the standard method. The norms validity was tested using independent groups of published data from factorial experiments with significant responses for N, P and K. For most data grouping, correct diagnosis were obtained using DRIS, while the method of critical values was inefficient for the N, P and K diagnosis.

In another paper, Angeles et al. (1993) developed DRIS norms for banana, based on 915 observations from 26 sources (published and unpublished data). The reference subpopulation was selected according to productivity equal or superior to 70 t ha-1. The indexes originated from the developed norms were compared with the method of critical values and the results of both methods were similar, except for K and K/nutrient ratios. The DRIS norms validity and their advantages over the method of critical values, by providing correct nutritional diagnosis, were partially confirmed through a fertilization experiment. In Eastern Africa, experiments and research carried out in 45 farms in the region of Kagera, Tanzania, also derived new norms to estimate the nutritional status of the banana plantation, using both DRIS and the critical value method (Wortmann et al., 1994).

These two methods were also utilized to identify nutrient deficiencies in papaya grown in nutrient solution in the greenhouse and in the field, in Hawaii, USA (Bowen, 1992). Plants grown in complete nutrient solution in the greenhouse did not show any nutrient deficiency, and the DRIS indexes were all positive, indicating that no nutrient was actually limiting plant growth. Plants grown in the field were found to present some visual symptoms of nutrient deficiency and their leaf petiole contents indicated deficient nutrient concentrations that according to DRIS were P, Fe and Zn. The average P concentration was below the critical level in all plants showing visual symptoms of nutrient unbalance, what was confirmed by the negative DRIS index. The indexes for Fe and Zn were also negative, indicating that they might not be in adequate levels, although the actual leaf Fe and Zn concentrations were within or above the sufficiency range. Toxicity might be, thus, an important factor expressing visual symptoms, particularly for Zn. Magnesium concentrations were significantly higher in unhealthy plants than in the healthy ones, but the DRIS indexes did not detect this situation. It was concluded that the DRIS method seems to be useful in detecting nutrient deficiencies in papaya, when used simultaneously with the method of critical values for the interpretation of tissue nutrient analysis.

For citrus, early studies on DRIS were carried out at California, USA, by Beverly et al. (1984), when preliminary reference values were derived for nutritional diagnosis of N, P, K, Ca, and Mg for Valencia' sweet orange. These values were also used for subsequent comparisons with the SRA and, overall, both methods presented similar results. However, the DRIS diagnosis was affected by the sample tissue type and maturation, and the indexes reflected the nutrient concentration change related to the yield alternation or to the presence of fruits in the shoots at sampling time. The DRIS indexes were in agreement with the SRA diagnosis, only when changes in nutrient concentration significantly affected the second method.

In a subsequent work, Beverly (1987) suggested three modifications on the DRIS method and proposed two new methods for nutritional diagnosis for Valencia' sweet orange. The logarithmic transformation, the use of standard populations and the adoption of an unique calculus procedure are modifications introduced to avoid systematic errors and simplify the diagnosis method, broadening its application. The two new suggested methods were based on individual plant nutrient concentrations, instead of nutrient ratios. The diagnosis resulted similar to the one obtained by DRIS or SRA, but provided more precise recommendations when evaluated by field tests. After this work, new researches involving data collecting during five more years revealed that SRA could be more advantageous than DRIS for Valencia' sweet orange (Beverly, 1992). The author compared SRA, DRIS and three modifications of DRIS. The SRA showed efficacy (not presenting false diagnosis) for N and P diagnosis status in 75 and 90% of the cases, respectively, compared to 50% or less by the other methods. All tested methods showed efficacy for K diagnosis.

Wallace (1990) carried out studies on DRIS for Valencia' sweet orange, from the established by Beverly et al. (1984), investigating several N, P, K ratios and interactions. This author observed 23 % yield increase in response to K supply, but 69 % increase when N and P were also added. DRIS was an effective method for nutritional diagnosis in this study. Woods & Villiers (1992), in research works developed at South Africa, obtained well succeeded DRIS results for Valencia' sweet orange, in disagreement with the results reported by Beverly (1992). Those authors observed good correlation between yield (kg plant-1) and fruit quality (fruit mass; g), with DRIS indexes derived from 1,700 observations. The results were compared with the conventional diagnosis method. The DRIS norms were also evaluated in fertilization experiments and the increase in yield and fruit quality (fruit mass) were consistent with DRIS diagnosis.

To develop DRIS norms for Verna' lemon nutritional diagnosis, research was carried out at Murcia and Alicante, Spain (Cerda et al., 1995). The adopted reference population presented yield equal or above 125 kg plant-1. The DRIS determinations were influenced by the rootstock/scion combination and leaf sampling period. The results of diagnosis agreed with those obtained by the SRA only when the analyzed leaves came from the same period of sampling than the ones for the DRIS norms. Under salinity conditions, DRIS was not effective in detecting if the cause of nutrient deficiency, that is, whether the nutrient unbalance was due to high salinity of fertilization deficiency. Results obtained in hydroponics were used to establish a data bank for DRIS indexes calculation for several citrus rootstock/scion combinations in Spain (Moreno et al., 1996). Useful reference values were determined for Fe availability evaluation and its influence in the nutrition of studied citrus rootstock/scion combinations, under sufficient and deficient Fe supply. A lemon scion budded on Citrus macrophylla rootstock showed less Fe-chlorosis deficiency symptoms compared to the same lemon budded on sour orange. Citrus volkameriana induced higher Fe-deficiency tolerance than Cleopatra mandarin, when used as rootstocks combined with sweet orange scions.

DRIS norms were developed for Valencia' sweet orange for a plant population with different plant ages, on various rootstocks, at several regions of the four most important citrus producing States of Venezuela (Rodriguez et al., 1997). The reference population was obtained through the selection of the 20% most productive plants. The values obtained were comparable to the previously determined in the literature. The authors concluded that DRIS method might be a low cost, timesaving and trustful alternative for the development of nutritional diagnosis norms.

In Brazil, there is paucity on DRIS method investigations, especially in fruit crops. Apart of one research carried out in banana (Teixeira et al., 2002), a few other studies were realized in citrus. Bataglia (1989) was probably the first author to report the application of this method for citrus nutritional diagnosis and indicated DRIS as an alternative diagnosis method, pointing out the need of using it together with other diagnoses criteria. The DRIS norms for N, P, K, Ca, Mg and S, were calculated according to Jones (1981), using a reference subpopulation with productivity equal or superior to 120 kg plant-1.

Creste (1996) reported the first DRIS evaluation by comparison with the SRA in groves of Brazil, studying Siciliano' lemon. Data were obtained from the analysis of leaves of fruiting branches of different plant ages and rootstocks, collected in several harvesting years. The reference population was derived from plants with productivity greater than 80 t ha-1. After the DRIS norms calculations, the method was evaluated under field conditions. DRIS showed to be more advantageous over the SRA, mainly because it was able to discriminate the nutrient importance order of deficiency or excess.

Santos (1997) evaluated the DRIS method using results of leaf analysis derived from a series of field experiments with N, P, K fertilization in commercial groves of the State of São Paulo. This author obtained superior results with the DRIS compared to the SRA, for detecting yield limitation by nutrient deficiency. Among the three available procedures for the DRIS indexes calculations, that proposed by Jones (1981) was the most advantageous.

Yield response curves were established for three cultivars of sweet orange (Pera', Valência' and Natal') budded on two rootstocks (Rangpur lime and Cleopatra mandarin) in southwestern State of São Paulo (Creste & Grassi Filho, 1998). The most productive rootstock/scion combination was obtained for Pera' sweet orange budded on Rangpur lime. It was suggested that regional DRIS norms should be established instead of general norms.

Mourão Filho & Azevedo (2003) established DRIS norms for the Valencia' sweet orange budded on Rangpur lime, Caipira sweet orange, and Poncirus trifoliata rootstocks. The nutritional balance indexes calculated by the derived norms were highly correlated with yield for the rootstock/scion combinations, from what it was inferred that DRIS norms might be applicable always that leaf sampling is collected from non-bearing fruit branches of irrigated-plant groves.

3 - Final remarks

A careful overview on the scientific literature reveals that DRIS is a promising, effective auxiliary tool for the nutritional diagnosis in several crops, although, still unknown, barely studied and applied. Except for a few studies, most of the developed research works turns clear that DRIS is as effective as the conventional methods of nutritional diagnosis (critical values and sufficiency range) with the additional advantage of establishing a nutrient deficiency or excess ranking, according to its importance, and a strong relation among them, quantifying the plant nutrient balance.

There are controversies regarding calculation procedures for the norms and DRIS indexes. One of the main questions is about the method application validation and the data universe that the norms are expected or supposed to represent. Most research results have indicated that the more specific the data universe, the more effective the method for the norms derivation.

The criteria for the reference subpopulation definition also demand further studies, and are, to a certain extent, specifically adjusted for each situation. In this way, DRIS norms should be developed for specific conditions, in which all other factors to be correlated with yield or quality (or any other variable) be known and isolated: cultivar, climate, soil and crop management, productivity etc., attaining the specific objectives.

Finally, it is highlighted that researches, both in a worldwide or Brazilian basis, on DRIS method utilization are incipient. Further investigations are necessary on the identification and isolation of factors that significantly affect productivity, under several fruit crop management production systems.

Accepted August 05, 2004

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# Publication Dates

• Publication in this collection
20 Sept 2004
• Date of issue
Oct 2004

• Accepted
05 Aug 2004