PHYSICOCHEMICAL CHARACTERISTICS AND FATTY ACID COMPOSITION OF TOMATO SEED OILS FROM PROCESSING WASTES

The major component of tomato processing industry wastes is seed. Samples of tomato (Petomech var.) pomace from industries of São Paulo state submitted to Hot and Cold Break treatments, were spontaneously fermented and washed to separate seeds. The oils were analysed for specific gravity, iodine and saponifícation numbers, refractive index, viscosity and fatty acid composition. Except for saponifícation number, Hot and Cold Break seed oils were very similar. In both treatments palmitic acid was the major saturated fatty acid, followed by stearic acid. Linoleic acid was the major unsaturated fatty acid followed by oleic acid. Both oleic and linoleic acids added up to over 60% of total fatty acids, being higher in Cold Break seed oils. Both treatments produced high nutritional oil quality. Differences in colour and viscosity may incur in more intense refining operations for Brazilian tomato processing industries due to the adoption of the Hot Break treatment.


INTRODUCTION
The expanding tomato processingindustry has been facing severe economical and technical environmental problems with the handling and disposal of resulting wastes.About one third of the total tomato production of 2.8 million tons goes to the industry for processing.The major component of such wastes is seed whose composition has been studied by several researchers (GAD et al., 1968;TSATSARONIS;BOSKOU, 1975;KRAMER;KWEE, 1977b;CANELLA et al., 1979;BRODOWSKI;GEISMAN, 1980;LAZOS;KALATHENOS, 1988;CANTARELLI et al., 1989), with data ranging from 14.6 to 29.6% crude fat; 14.8 to 41.8% crude fibre; 2.0 to 9.6% ash; 2.9 to 5.4% carbohydrates and 22.9 to 36.8%  1983a,b).The lipid fraction showed a high degree of unsaturation, with up to 55% linoleic acid (LAZOS; KALATHENOS, 1988).
The purpose of this work was to determine the fatty acid composition and some physicochemical characteristics of the oils extracted from industrial tomato seed wastes and evaluate its potential for human consumption.

MATERIAL AND METHODS
Three samples of 10kg tomato pomace were obtained after the commercial extraction of ripe tomatoes (cultivar Petomech) grown in São Paulo State, Brazil, which were submitted to Hot Break (95°C/10 min) or Cold Break (60°C/10 min) treatments.The samples, after a spontaneous fermentation at room temperature for ca.one week, were washed with tap water to separate seeds from skins and gelatinous material.The seeds were dried at 45°C for 48 hr in a forced air oven, and then comminuted with a grinder model 4-E (Quaker City Mill, Pa).
Seed oils were extracted with n-hexane (boiling point 68-70°C, 6 hr) in a Soxhlet extractor, and stored in glass vials at 1-5°C.Methyl esters of the fatty acids of the tomato oils were prepared according to LUDDY et al. (1960).The gas chromatographic analysis was perfomed with a C.G. 37-D Gas-Chromatograph (C.G.Scientific Instr., Brazil) fitted with a single stainless steel column (200 X 0.95 cm) packed with 18% Diethyleneglycol succinate (DECS) on Chromosorb P A/W DMCS and flame ionization detector.Operating conditions were as follows: column temperature, 170° C; detector temperature, 250°C: injector temperature, 210°C and nitrogen flow rate, 50 ml/min.
Fatty acid identification was based on chromatograms of known standards and by the Equivalent Chain Length Method.Peak areas were measured by triangulation.
Oil characteristics, namely specific gravity, iodine and saponification numbers, were performed according to standard methods (Association of Official Analytical Chemistry, 1975).Refractive index was determined by Abbe refractometer at 20°C.The viscosity was determined with an Epprechet viscosimeter model TVB (Contraves Inf.Products, England).All data represent mean values of three determinations not exceeding * 5% variation.

RESULTS AND DISCUSSION
The fatty acid composition is presented in TABLE 1. Saturated, short-chained fatty acids except for stearic and behenic acids presented lower contents in the Cold Break seed oil.
Oleic and linoleic acid contents were higher in the Cold Break seed oil, while linoleic acid content was lower than that in the Hot Break seed oil.
Total saturated fatty acid content was higher in the Hot Break seed oil while total unsaturated fatty acid content was higher for the Cold Break seed oil.
On a percentage basis, it is known that such thermal treatments provide different yields of oil contents (CANTARELLI et al., 1989), i.e. 19.0 and 14.5% for Cold and Hot Break treatments, respectively.Therefore one could suppose there has been a differential leaching of oil (stearic and oleic acids being the most affected) and other components (protein, amino acids and carbohydrates) during the Hot Break treatment as shown in the previous work.
In both treatments, palmitic acid was the major saturated fatty acid, followed by stearic acid.Linoleic acid was the major unsaturated fatty acid followed by oleic acid.This data is in accordance with BERTONI et al. (1963);GAD et al. (1968); TSATSARONIS; BOSKOU (1972); VIGO et al. (1977);CANELLA et al. (1979) and LAZOS;KALATHENOS (1988).The differences in individual contents of fatty acids when compared to the literature, may be due to the cultivars used and to the cultivation and/or environmental factors.
As can be observed from TABLE 2, except for the saponification number, Hot and Cold Break seed oils were very similar.Compared to other authors' data they can be included within the range of values reported.The levels found were in agreement with CANELLA et al. (1979), who worked with the same cultivar (Petomech).They showed a high degree of unsaturation and the presence of long chain fatty acids.
Cold Break seed oil was clearer golden yellow in colour and less viscous, 57.5 cP at 25°C, than the Hot Break seed oil which presented a darker red color and 61.0 cP viscosity at the same temperature.Brazilian tomato processing industries use the Hot Break treatment for enzymic inactivation which would mean more intense refining operations to obtain a commercial oil quality.

CONCLUSIONS
The present work confirms previous information by CANTARELLI et al. (1989) that indicates that tomato seed wastes are a potential source of edible oil.They should not be discarded as a fertilizer or animal feed due to the high unsaturated fatty acid content of the oils, whether they originate from Cold or Hot Break treatments of the ripe tomatoes.They are good sources of the essential linoleic fatty acid.