ABSTRACT
The aim this study was to characterize four isolates of Diplodia pinea from the South region of Brazil and to estimate their genetic diversity, based on vegetative compatibility and RAPD markers. For vegetative compatibility, isolates were paired on Petri dishes containing PDA medium and formed dark lines when incompatible and cottony light lines when compatible. For the molecular characterization of isolates, DNA extraction was performed for samples obtained from mycelium grown on PDA medium of original and derived monosporic isolates. The DNA of samples was assessed according to polymerase chain reaction (PCR), using eleven different sequences of nonspecific RAPD primers. The grouping of morphotypes was performed according to the UPGMA method and the Jaccard similarity coefficient. Only one RAPD marker showed polymorphism, indicating that the genetic diversity of isolates is low but enough to indicate more than the one present morphotype.
Keywords
genetic diversity; morphotype; Pinus; shoot dieback
