The detection, transmission and the effect of Xanthomomas campestris pv. campestris (Xcc) in the physiological quality of broccoli seeds were evaluated. The seeds were obtained from inoculated field plants (`Baron', `Flórida', `Hana Midori Sakata', `Precoce Piracicaba de Verão', `Ramoso Santana' and `Sabre' genotypes) with the bacterium. For the seed pathogen detection the semi-selective medium were used: starch for xanthomonads (SX) agar, nutrient starch cycloheximide antibiotic agar (NSCAA) and basal starch cycloheximide antibiotic agar (BSCAA); the bacteria seeds transmission percentage was evaluated using sand and test tube. The physiological seed quality was evaluated by the standard germination and vigor tests: the accelerated aging, speed of emergence, seedling growth and seedling dry weight. The semi-selective medium NSCAA was more efficient to detect Xcc from broccoli seeds; there was not significant difference among genotypes regarding the percentage of bacteria transmissed by seeds percentage; although, Xcc can be disseminated by seeds, it did not affect both seed germination and vigor.
black rot; seed pathology; Brassica oleracea var; italica
