This experiment was carried out in the Laboratory of Phytopathology - Mycology - School of Agronomy and Veterinary Medicine, Passo Fundo University, in order to evaluate Corynespora cassiicola sporulation on different substrates. Thus the used Petri plates contained six different culture media - Potato Dextrose Agar (PDA), Czapek Solution Agar, infant food, Malt Agar, Oatmeal Agar, and V8 juice agar, besides four combinations (with both light and filter paper, with light and without filter paper, with filter paper and without light, and without light and without filter paper). Experimental design was triple factorial (substrate, light / dark, with or without filter paper). In the fungus sporulation assessment, two disks (0241cm²) were cut from each plate of the different substrates and transferred to test tubes containing 10mL distilled water. These tubes were shaken: then, three 10mL aliquots were removed for conidia counting under optical microscope, The obtained data were converted into number of spores.cm². The highest fungus sporulation was obtained with the substrate Czapek Solution Agar under 12h-light photoperiod and filter paper overlapping.
Inoculum production; culture medium; photoperiod
