ABSTRACT
Among the fungal diseases of vine, leaf spot caused by Pseudocercospora vitis is worthy of note. The favorable conditions for the development of leaf spot have not been fully determined yet. Conidia are known to be formed under high humidity conditions and spread by the wind, initiating new infections. To increase the knowledge on production, germination and infection by this pathogen, three experiments were conducted. In the first experiment, we evaluated the production of P. vitis spores on the culture media: PDA (potato dextrose agar); PDA + coconut milk (5%); PDA + 'Isabel cv.' grape extract (5%); ST (tomato juice-calcium carbonate-agar); ST + coconut milk (5%); ST + 'Isabel cv.' grape extract (5%). There was sporulation on all tested media and different incubation periods. In the second experiment, the temperature of 25°C provided higher percentage of germinated conidia and there was greater germination in the absence of light. In the third experiment, the temperature of 20°C led to greater severity of the leaf spot in vine seedlings after 48 hours of incubation in the dark.
Additional keywords:
epidemiology; conidium; grape; leaf spot
