Acta Cirúrgica Brasileirahttps://www.scielo.br/journal/acb/feed/2024-02-02T20:04:41.796000ZVol. 39 - 2024WerkzeugCombined administration of gallic acid and glibenclamide mitigate systemic complication and histological changes in the cornea of diabetic rats induced with streptozotocin10.1590/acb3901242024-02-02T20:04:41.796000Z2020-08-09T06:48:06.918000ZZhao, JingHussain, Shaik AlthafMaddu, Narendra
<em>Zhao, Jing</em>;
<em>Hussain, Shaik Althaf</em>;
<em>Maddu, Narendra</em>;
<br/><br/>
ABSTRACT Purpose: To determine the effect of gallic acid or its combination with glibenclamide on some biochemical markers and histology of the cornea of streptozotocin (STZ) induced diabetic rats. Methods: Following induction of diabetes, 24 male albino rats were divided into four groups of six rats each. Groups 1 and 2 (control and diabetic) received rat pellets and distilled water; group 3 (gallic acid) received rat pellets and gallic acid (10 mg/kg, orally) dissolved in the distilled water; and group 4 (gallic acid + glibenclamide) received rat pellets, gallic acid (10 mg/kg, orally), and glibenclamide (5 mg/kg, orally) dissolved in the distilled water. The treatments were administered for three months after which the rats were sacrificed after an overnight fast. Blood and sera were collected for the determination of biochemical parameters, while their eyes were excised for histology. Results: STZ administration to the rats induced insulin resistance, hyperglycemia, microprotenuria, loss of weight, oxidative stress, inflammation, and alteration of their cornea histology, which was abolished following supplementation with gallic acid or its combination with glibenclamide. Conclusions: The study showed the potentials of gallic acid and glibenclamide in mitigating systemic complication and histological changes in the cornea of diabetic rats induced with STZ.Flap of the sternocephalicus muscle in the repair of a partial defect in the trachea of a rabbit (<i>Oryctolagus cuniculus</i>)10.1590/acb3903242024-02-02T20:04:41.796000Z2020-08-09T06:48:06.918000ZFernandes, GustavoRein, AriadneMontanhim, Gabriel LuizCosta, Marcelo Carrijo daLeite, Marcella Dall’AgnolSoares, Nicolle PereiraMoraes, Paola Castro
<em>Fernandes, Gustavo</em>;
<em>Rein, Ariadne</em>;
<em>Montanhim, Gabriel Luiz</em>;
<em>Costa, Marcelo Carrijo Da</em>;
<em>Leite, Marcella Dall’agnol</em>;
<em>Soares, Nicolle Pereira</em>;
<em>Moraes, Paola Castro</em>;
<br/><br/>
ABSTRACT Purpose: The current study aimed at evaluating the repair of a partial defect of the trachea with a muscle flap, an advanced technique that employs combined suture patterns. Methods: Sixteen healthy male New Zealand white rabbits were used as an experimental model. A partial defect in the trachea within the ventral region of the fourth to eighth tracheal ring was created. Subsequently, repair was initiated with a flap of the sternocephalicus muscle. The animals were divided into four groups for postoperative evaluation using clinical, tracheoscopic, and histopathological analyses. Each group was separated according to the time of euthanasia, programmed at interval of seven (G7), 15 (G15), 30 (G30), and 60 days (G60). Results: One animal from the G60 group died, whereas the other animals had good surgical recovery without serious changes in the breathing pattern. The major clinical signs observed were stridor and coughing. Tracheoscopy revealed secretions in the tracheal lumen, exuberant granulation, and stenosis. Histopathological analysis showed growth of the ciliary respiratory epithelium at the flap site 30 days after implantation. Conclusions: Partial repair showed satisfactory results owing to the anatomical location of the muscle, adequate vascular support, and structural and physiological maintenance without serious changes in the respiratory system.Effects of rosmarinic acid and doxorubicine on an ovarian adenocarsinoma cell line (OVCAR3) via the EGFR pathway10.1590/acb3905242024-02-02T20:04:41.796000Z2020-08-09T06:48:06.918000ZSarı, UmutZaman, Fuat
<em>Sarı, Umut</em>;
<em>Zaman, Fuat</em>;
<br/><br/>
ABSTRACT Purpose: We aimed to reveal the effects of rosmarinic acid (RA), which has come to the forefront with its antitumor and antioxidant properties in many studies recently in the ovarian adenocarcinoma cell line, on the epidermal growth factor receptor (EFGR) signaling pathway in the presence of doxorubicin (DOX). Methods: Ovarian adenocarcinoma cell line (OVCAR3) and human skin keratinocyte cell line human skin keratinocyte cell line (HaCaT) were used as control. (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test was applied to determine the effect of RA and DOX on the proliferation of OVCAR3 and HaCaT cells. Bcl2 expression and epidermal growth factor receptor (EGFR) and western blot analysis were performed to determine the expression levels of the markers. Results: It was determined that RA (IC50 = 437.6 μM) and DOX (IC50 = 0.08 μM) have the ability to inhibit the proliferation of OVCAR3 cells and induce apoptosis in a 72-hour time and dose-dependent manner. Western blot showed that the expression level of Bcl-2 and EGFR in OVCAR3 cells was down-regulated by RA and DOX. Conclusions: Apoptosis in OVCAR3 cells can potentially be induced by RA via the EGFR pathway, and RA may be a potent agent for cancer therapy.Bone protective effect of sinomenine against monosodium iodoacetate induced knee and hip injury in rat model: an inflammatory pathway10.1590/acb3909242024-02-02T20:04:41.796000Z2020-08-09T06:48:06.918000ZLei, Yi-HaoHu, Xing-XiWen, Hong-JieDeng, Yong-ChengJiang, Jun-LiangZhao, Qing-Gang
<em>Lei, Yi-Hao</em>;
<em>Hu, Xing-Xi</em>;
<em>Wen, Hong-Jie</em>;
<em>Deng, Yong-Cheng</em>;
<em>Jiang, Jun-Liang</em>;
<em>Zhao, Qing-Gang</em>;
<br/><br/>
ABSTRACT Purpose: Osteoarthritis (OA) is a degenerative joint disease which is categorized via destruction of joint cartilage and it also affects the various joints, especially knees and hips. Sinomenine active phytoconstituents isolated from the stem of Sinomenium acutum and already proof anti-inflammatory effect against the arthritis model of rodent. In this experimental protocol, we scrutinized the anti-osteoarthritis effect of sinomenine against monosodium iodoacetate (MIA) induced OA in rats. Methods: MIA (3 mg/50 μL) was used for inducing the OA in the rats, and rats received the oral administration of sinomenine (2.5, 5 and 7.5 mg/kg body weight) up to the end of the experimental study (four weeks). The body and organs weight were estimated. Aggrecan, C-terminal cross-linked telopeptide of type II collagen (CTX-II), glycosaminoglycans (GCGs), monocyte chemoattractant protein-1 (MCP-1), Interferon gamma (IFN-γ), antioxidant, inflammatory cytokines, inflammatory mediators and matrix metalloproteinases (MMP) were analyzed. Results: Sinomenine significantly (P < 0.001) boosted the body weight and reduced the heart weight, but the weight of spleen and kidney remain unchanged. Sinomenine significantly (P < 0.001) reduced the level of nitric oxide, MCP-1 and improved the level of aggrecan, IFN-γ and GCGs. Sinomenine remarkably upregulated the level of glutathione, superoxide dismutase and suppressed the level of malonaldehyde. It effectually modulated the level of inflammatory cytokines and inflammatory mediators and significantly (P < 0.001) reduced the level of MMPs, like MMP-1, 2, 3, 9 and 13. Conclusions: Sinomenine is a beneficial active agent for the treatment of OA disease.Breviscapine alleviates myocardial ischemia-reperfusion injury in diabetes rats10.1590/acb3902242024-02-02T20:04:41.796000Z2020-08-09T06:48:06.918000ZSu, ZhenhongZheng, YuanmeiHan, MengZhao, DeqingHuang, ZhiZhou, YijunHu, Wenbing
<em>Su, Zhenhong</em>;
<em>Zheng, Yuanmei</em>;
<em>Han, Meng</em>;
<em>Zhao, Deqing</em>;
<em>Huang, Zhi</em>;
<em>Zhou, Yijun</em>;
<em>Hu, Wenbing</em>;
<br/><br/>
ABSTRACT Purpose: To investigate the protective effect of breviscapine on myocardial ischemia-reperfusion injury (MIRI) in diabetes rats. Methods: Forty rats were divided into control, diabetes, MIRI of diabetes, and treatment groups. The MIRI of diabetes model was established in the latter two groups. Then, the treatment group was treated with 100 mg/kg breviscapine by intraperitoneal injection for 14 consecutive days. Results: After treatment, compared with MIRI of diabetes group, in treatment group the serum fasting blood glucose, fasting insulin, homeostasis model assessment of insulin resistance, and glycosylated hemoglobin levels decreased, the serum total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol levels decreased, the serum high-density lipoprotein cholesterol level increased, the heart rate decreased, the mean arterial pressure, left ventricular ejection fraction, and fractional shortening increased, the serum cardiac troponin I, and creatine kinase-MB levels decreased, the myocardial tumor necrosis factor α and interleukin-6 levels decreased, the myocardial superoxide dismutase level increased, and the myocardial malondialdehyde level decreased (all P < 0.05). Conclusions: For treating MIRI of diabetes in rats, the breviscapine can reduce the blood glucose and lipid levels, improve the cardiac function, reduce the myocardial injury, and decrease the inflammatory response and oxidative stress, thus exerting the alleviating effect.Maturation and development of fetal pig intestinal tissue in immunodeficient mice10.1590/acb3906242024-02-02T20:04:41.796000Z2020-08-09T06:48:06.918000ZHarada, AtsushiMatsumoto, NaotoKinoshita, YoshitakaMatsu, KenjiInage, YukaMorimoto, KeitaYamanaka, ShuichiroKurobe, MasashiYokoo, TakashiKume, HarukiOhki, TakaoKobayashi, Eiji
<em>Harada, Atsushi</em>;
<em>Matsumoto, Naoto</em>;
<em>Kinoshita, Yoshitaka</em>;
<em>Matsu, Kenji</em>;
<em>Inage, Yuka</em>;
<em>Morimoto, Keita</em>;
<em>Yamanaka, Shuichiro</em>;
<em>Kurobe, Masashi</em>;
<em>Yokoo, Takashi</em>;
<em>Kume, Haruki</em>;
<em>Ohki, Takao</em>;
<em>Kobayashi, Eiji</em>;
<br/><br/>
ABSTRACT Purpose: This study aimed to compare the degree of maturation and development of fetal pig segmental intestinal tissue with that of spheroids created by in-vitro reaggregation of dissociated fetal intestinal cells after transplantation into immunodeficient mice. Methods: Fetal pig small intestines were transplanted as segmental grafts into the omentum and subrenal capsules of immunodeficient mice or enzymatically treated to generate single cells. Spheroids made by in-vitro reaggregation of these cells were transplanted into the subrenal capsules of immunodeficient mice. The segmental grafts and spheroids were harvested four and eight weeks after transplantation, and the structural maturity and in-vivo development of these specimens were histologically evaluated. Results: The spheroids were engrafted and supplied blood vessels from the host mice, but an intestinal layered structure was not clearly observed, and there was almost no change in size. On the other hand, the segmental grafts formed deep crypts in the mucus membrane, the inner circular layer, and outer longitudinal muscles. The crypts of the transplanted grafts harvested at eight weeks were much deeper, and the smooth muscle layer and the enteric nervous system were more mature than those of grafts harvested at the fourth week, although the intestinal peristaltic wave was not observed. Conclusions: Spheroids created from fetal small intestinal cells could not form layered structures or mature sufficiently. Conversely, segmental tissues structurally matured and developed after in-vivo transplantation and are therefore potential grafts for transplantation.The effect of biopolymer gel derived from sugarcane on healing of traumatic oral ulcers: an experimental study10.1590/acb3907242024-02-02T20:04:41.796000Z2020-08-09T06:48:06.918000ZBarbosa, Lívia MirelleOliveira, Lívia Maria Lopes deQueiroz, Camylla Pinheiro VerissimoFreitas, Bruna Andrade SantosLima, Tarciana Maria Pereira deAguiar, José Lamartine de AndradeMedeiros Júnior, Martinho DinoáPerez, Danyel Elias da CruzGodoy, Gustavo Pina
<em>Barbosa, Lívia Mirelle</em>;
<em>Oliveira, Lívia Maria Lopes De</em>;
<em>Queiroz, Camylla Pinheiro Verissimo</em>;
<em>Freitas, Bruna Andrade Santos</em>;
<em>Lima, Tarciana Maria Pereira De</em>;
<em>Aguiar, José Lamartine De Andrade</em>;
<em>Medeiros Júnior, Martinho Dinoá</em>;
<em>Perez, Danyel Elias Da Cruz</em>;
<em>Godoy, Gustavo Pina</em>;
<br/><br/>
ABSTRACT Purpose: The extracellular polysaccharide (EPS) is produced by the bacterium Zoogloea sp. and plays a positive role in tissue repair. The purpose of this study was to clinically and histologically compare the effects of EPS in the healing of traumatic oral ulcers in rats with the effects of triamcinolone. Methods: Ulcers were induced in the oral mucous of 45 male Wistar rats, divided into three groups: control group, treated with triamcinolone, and treated with biopolymer gel. In the clinical evaluation, we considered the weight variation of the animals and the size of the lesion area, at baseline and on treatment days 1, 3 and 7. The histological parameters evaluated were the type and intensity of the inflammatory infiltration, the presence of necrosis and foreign body granuloma and the degree of re-epithelialization of the lesion. Results: The reduction of the lesion area was greater in the animals treated with EPS, with no difference in the intensity of the inflammatory infiltration between the groups on days 3 and 7 of treatment. Conclusions: The results suggest that topical application of EPS in traumatic oral ulcers of rats promotes faster repair than triamcinolone ointment, without increasing the intensity of inflammatory infiltration under the lesion.Protective effects of hesperidin in gastric damage caused by experimental ischemia-reperfusion injury model in rats10.1590/acb3911242024-02-02T20:04:41.796000Z2020-08-09T06:48:06.918000ZOzyigit, FilizDeger, Ayse NurKocak, Fatma EmelEkici, Mehmet FatihSimsek, HasanArık, Ozlem
<em>Ozyigit, Filiz</em>;
<em>Deger, Ayse Nur</em>;
<em>Kocak, Fatma Emel</em>;
<em>Ekici, Mehmet Fatih</em>;
<em>Simsek, Hasan</em>;
<em>Arık, Ozlem</em>;
<br/><br/>
ABSTRACT Purpose: This study evaluated the protective effect of hesperidin on injury induced by gastric ischemia-reperfusion. Methods: Fifty male Sprague Dawley rats (250–300 g) were divided into five groups: control (C), sham (S), ischemia (I), ischemia-reperfusion (I/R) and hesperidin + ischemia-reperfusion (Hes + I/R). Hesperidin was injected intraperitoneally at the dose of 100 mg/kg one hour before the experimental stomach ischemia-reperfusion. Celiac artery was ligated. After 45 minutes ischemia and 60 minutes reperfusion period, blood samples were obtained under anesthesia. Then, animals were sacrificed, stomach tissues were excised for biochemical, and histopathological analyses were performed. Malondialdehyde levels and superoxide dismutase, glutathione peroxidase activities and total antioxidant status (TAS), total oxidant status (TOS), protein, total thiol parameters were measured in plasma, and tissue homogenate samples. H + E, periodic acid–Schiff, hypoxia inducible factor, terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end-labeling (TUNEL), and proliferating cell nuclear antigen (PCNA) for cell proliferation as immunohistochemical parameters were determined. Results: Upon biochemical and histopathological assessment, hesperidin decreased stomach tissue changes in comparison with IR group. Ischemia-reperfusion injury led to a considerably increase in malondialdehyde, protein, and TOS levels (p < 0.001) in stomach tissue. Hesperidin treatment significantly decreased malondialdehyde, protein, and TOS levels (p < 0.001). Hesperidin increased superoxide dismutase, TAS, total thiol and glutathione peroxidase activities in comparison with IR group. Hesperidin reduced damage and also increased TUNEL and PCNA immunoreactivity in stomach tissue. Conclusions: Hesperidin was able to decrease I/R injury of the stomach tissue due to inhibition of lipid peroxidation and protein oxidation, duration of antioxidant, and free radical scavenger properties. Consequently, hesperidin can provide a beneficial therapeutic choice for preventing stomach tissue ischemia-reperfusion injury in clinical application.Ellagic acid protected the gingival tissue via fibroblast and epidermal growth factors in rats10.1590/acb3912242024-02-02T20:04:41.796000Z2020-08-09T06:48:06.918000ZTekin, Gülüçağ GirayDeveci, BuşraDeveci, Engin
<em>Tekin, Gülüçağ Giray</em>;
<em>Deveci, Buşra</em>;
<em>Deveci, Engin</em>;
<br/><br/>
ABSTRACT Purpose: To investigate the effect of ellagic acid (EA) in gingival tissues injury in rats. Methods: Twenty rats were categorized into two groups. In burn group, an excisional wound area was created by removing a 4-mm diameter flap from the left molar region in the mucoperiosteal region of the gingiva. In burn + ellagic acid group, 1.2 mg/mL EA was administered as irrigation for one week. Animals was sacrificed under anesthesia at the end of experiment. Malondialdehyde (MDA), myeloperoxidase (MPO) and glutathione (GSH) level were measured. Hematoxylin and eosin, fibroblast growth factor (FGF) and epidermal growth factor (EGF) immunostainings were applied to tissues. Results: MDA, MPO, inflammation and leukocyte infiltration were high in burn group. Degeneration epithelium, edema and inflammatory cell infiltration in connective tissue areas, and dilatation and congestion in blood vessels were observed in burn group. In burn + EA group, the gingival epithelium improved, collagen fiber production increased and organized dermis were observed. After burn injury, FGF and EGF activity was increased in EA treated groups. Conclusions: We suggest that EA have the potential for better healing outcomes in oral wounds. EA seems to have promising therapeutic efficacy to enhance oral wound healing.Establishment and application of a new 4/6 infarct nephrectomy rat model for moderate chronic kidney disease10.1590/acb3913242024-02-02T20:04:41.796000Z2020-08-09T06:48:06.918000ZSugai, KazuhisaHirano, MomokoOda, AsahiFujisawa, MasahikoShono, SaoriIshioka, KatsumiTamura, TomoyoshiKatsumata, YoshinoriSano, MotoakiKobayashi, EijiHakamata, Yoji
<em>Sugai, Kazuhisa</em>;
<em>Hirano, Momoko</em>;
<em>Oda, Asahi</em>;
<em>Fujisawa, Masahiko</em>;
<em>Shono, Saori</em>;
<em>Ishioka, Katsumi</em>;
<em>Tamura, Tomoyoshi</em>;
<em>Katsumata, Yoshinori</em>;
<em>Sano, Motoaki</em>;
<em>Kobayashi, Eiji</em>;
<em>Hakamata, Yoji</em>;
<br/><br/>
ABSTRACT Purpose: To develop a new 4/6 infarct nephrectomy (INx) model rat mimicking moderate chronic kidney disease (CKD) and to evaluate its application. Methods: We modified the conventional 5/6 INx rat model to create the 4/6 INx model by ligating the renal artery branch to induce infarction of one-third of the left kidney after right kidney removal and compared biochemically and histologically both models. To demonstrate the application of the 4/6 INx model, the effects of a supplementary compound containing calcium carbonate, chitosan, palm shell activated charcoal etc., that is effective for both CKD and its complications, were compared between both models. Results: Impairment of renal function in the 4/6 INx group was significantly more moderate than in the 5/6 INx group (P < 0.05). The 4/6 INx group showed less histological damage in kidney than in the 5/6 INx group. The supplementary compound did not improve CKD in the 5/6 INx group, but ameliorated elevation of blood urea nitrogen in the 4/6 INx group. Conclusions: We developed the 4/6 INx model, which is more moderate than the conventional 5/6 INx model. This model could potentially demonstrate the effectiveness of drugs and supplements intended to prevent CKD and its progression.XinJiaCongRongTuSiZiWan protects triptolide-induced rats from oxidative stress injury via mitophagy mediated PINK1/Parkin signaling pathway10.1590/acb3914242024-02-02T20:04:41.796000Z2020-08-09T06:48:06.918000ZJin, YanDi-si, DengKe-ming, Wu
<em>Jin, Yan</em>;
<em>Di-Si, Deng</em>;
<em>Ke-Ming, Wu</em>;
<br/><br/>
ABSTRACT Purpose: XinJiaCongRongTuSiZiWan (XJCRTSZW) is a traditional Chinese medicine compound for invigorating the kidney, nourishing blood, and promoting blood circulation. This study aimed to explore the effect of XJCRTSZW on triptolide (TP)-induced oxidative stress injury. Methods: Adult female Sprague-Dawley rats and human ovarian granulosa cell lines were treated with TP and XJCRTSZW. Hematoxylin and eosin staining, enzyme-linked immunosorbent assay, flow cytometry, CCK-8, JC-1 staining, transmission electron microscopy, reverse transcription-quantitative polymerase chain reaction, and Western blotting were performed in this study. Results: XJCRTSZW treatment observably ameliorated the TP-induced pathological symptoms. Furthermore, XJCRTSZW treatment observably enhanced the TP-induced reduction of estradiol, anti-Mullerian hormone, progesterone, superoxide dismutase, ATP content, mitochondrial membrane potential, p62, and Hsp60 mRNA, and protein levels in vivo and in vitro (p < 0.05). However, TP-induced elevation of follicle stimulating hormone and luteinizing hormone concentrations, malondialdehyde levels, reactive oxygen species levels, apoptosis rate, mitophagy, and the mRNA and protein expressions of LC3-II/LC3-I, PTEN-induced kinase 1 (PINK1), and Parkin were decreased (p < 0.05). In addition, XJCRTSZW treatment markedly increased cell viability in vitro (p < 0.05). Conclusions: XJCRTSZW protects TP-induced rats from oxidative stress injury via the mitophagy-mediated PINK1/Parkin pathway.Photobiomodulation effects on synovial morphology, iNOS gene, and protein expression in a model of acute inflammation10.1590/acb3920242024-02-02T20:04:41.796000Z2020-08-09T06:48:06.918000ZBomfim, Fernando Russo Costa doGomes, Bruna SilvaLanza, Sabrina ZanchettaEsquisatto, Marcelo Augusto MarrettoLopes-Filho, Gaspar de Jesus
<em>Bomfim, Fernando Russo Costa Do</em>;
<em>Gomes, Bruna Silva</em>;
<em>Lanza, Sabrina Zanchetta</em>;
<em>Esquisatto, Marcelo Augusto Marretto</em>;
<em>Lopes-Filho, Gaspar De Jesus</em>;
<br/><br/>
ABSTRACT Purpose: To evaluate morphological aspects and inducible nitric oxide synthase (iNOS) gene and protein expression in a model of acute inflammation. Methods: Thirty-six female Wistar rats were assigned into three groups: control (saline, n = 12), sham (arthritis, n = 12), and PBM (arthritis and photobiomodulation, n = 12). Arthritis induction was performed with 200 μg of intra-articular Zymosan in sham and PBM animals. PBM was performed 24 h after induction with a laser device (λ = 808 nm, 25 mW of nominal power, fluence of 20 J/cm2, beam area of 0.02 mm2, time of 33 s, total energy of 0.825 J) with punctual and single dose application. Morphological analysis of joint structure (HE) and immunohistochemistry (anti-iNOS antibody) were performed on knee samples, and synovial tissue was submitted to RNA extraction, cDNA synthesis and gene expression analysis by quantitative polymerase chain reaction. Statistical analyses were performed with p < 0.05. Results: It was observed an increase in the thickness of the synovial lining epithelium and inflammatory infiltrate in sham compared to PBM. Gene expression analysis showed higher iNOS expression in PBM, and iNOS protein expression decreased in PBM compared to sham. Conclusions: Photobiomodulation decreased inflammation in PBM animals, upregulated iNOS gene expression, however down egulated protein expression compared to sham.Antibiotics prophylaxis at the time of catheter removal after radical prostatectomy: a systematic review of the literature and meta-analysis10.1590/acb3904242024-02-02T20:04:41.796000Z2020-08-09T06:48:06.918000ZSantos, Letícia Lourenço dosFraga, Isabela de AlmeidaAlmeida, Vitor Amaral deSantos, Andressa Hellen RibeiroAlmeida, Isabelle MatosNascimento, Tatiana RobertaPorto, Breno CordeiroPasserotti, Carlo CamargoArtifon, Everson Luiz de AlmeidaOtoch, Jose PinhataCruz, José Arnaldo Shiomi da
<em>Santos, Letícia Lourenço Dos</em>;
<em>Fraga, Isabela De Almeida</em>;
<em>Almeida, Vitor Amaral De</em>;
<em>Santos, Andressa Hellen Ribeiro</em>;
<em>Almeida, Isabelle Matos</em>;
<em>Nascimento, Tatiana Roberta</em>;
<em>Porto, Breno Cordeiro</em>;
<em>Passerotti, Carlo Camargo</em>;
<em>Artifon, Everson Luiz De Almeida</em>;
<em>Otoch, Jose Pinhata</em>;
<em>Cruz, José Arnaldo Shiomi Da</em>;
<br/><br/>
ABSTRACT Purpose: To conduct a systematic literature review with meta-analysis to identify whether antibiotic prophylaxis after removal of the indwelling urinary catheter reduces posterior infections. Methods: A systematic literature review was conducted in the databases PubMed, Embase, Cochrane, Google Scholar, and Latin American and Caribbean Health Sciences Literature, using the keywords “antibiotics” AND “prostatectomy” AND “urinary catheter.” Results: Three articles were identified having the scope of our review, with 1,040 patients, which were subjected to our meta-analysis revealing a marginally significant decrease in the risk of urinary infection after indwelling urinary catheter removal (odds ratio–OR = 0.51; 95% confidence interval–95%CI 0.27–0.98; p = 0.04; I2 = 0%). No difference was found regarding the presence of bacteriuria (OR = 0.39; 95%CI 0.12–1.24; p = 0.11; I2 = 73%). Conclusions: In our meta-analysis, there was a significant decrease in urinary tract infection with antibiotic prophylaxis after indwelling urinary catheter removal following radical prostatectomy.Clinical behavior of recurrent pleomorphic adenoma in the palate: a systematic review10.1590/acb3908242024-02-02T20:04:41.796000Z2020-08-09T06:48:06.918000ZLopes-Santos, GabrielaMarques, Najara Gomes de OliveiraTjioe, Kellen CristineOliveira, Denise Tostes
<em>Lopes-Santos, Gabriela</em>;
<em>Marques, Najara Gomes De Oliveira</em>;
<em>Tjioe, Kellen Cristine</em>;
<em>Oliveira, Denise Tostes</em>;
<br/><br/>
ABSTRACT Purpose: This systematic review analyzed the clinical behavior and odds of malignancy of the palatal recurrent pleomorphic adenomas. Methods: Systematic review of patients with recurrent pleomorphic adenoma arising in the palate. Database search: MEDLINE, Scopus, Web of Science, Cochrane, EMBASE, Virtual Health Library, Google Scholar, and OpenGrey. A binomial logistic regression was performed to assess the odds of detecting recurrence five, 10 and 20 years after the treatment of primary tumor. Results: Thirteen studies (n = 18 patients) out of 336 were included. The recurrent pleomorphic adenoma in palate was more common in females (61.6%), average age was 49 years old (range 9–73 years old). Four patients progressed to malignant transformation. The odds ratio (OR) of detecting a recurrence at 10 (OR = 5.57; 95% confidence interval – 95%CI 1.13–27.52), and 20 years (OR = 18.78; 95%CI 3.18–110.84) after treatment of primary pleomorphic adenoma was significantly higher than at one-year follow-up. Conclusions: The recurrence of pleomorphic adenoma in palate remains a rare event of late occurrence. It mainly affects middle-aged female and carries a risk of malignant transformation. Although uncommon, patients with palatal pleomorphic adenoma should be warned about the possibility of recurrence or malignant transformation of tumor at advanced ages.