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vol.20 issue5Validated high-performance liquid chromatographic method for the standardisation of Ptychopetalum olacoides Benth., Olacaceae, commercial extractsEvaluation of PEG effects on the extracts obtaining from Passiflora edulis Sims, Passifloraceae, and Mikania glomerata Spreng., Asteraceae author indexsubject indexarticles search
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Revista Brasileira de Farmacognosia

Print version ISSN 0102-695X


LOPES, Gisely Cristiny et al. Development and validation of an HPLC method for the determination of epicatechin in Maytenus ilicifolia (Schrad.) Planch., Celastraceae. Rev. bras. farmacogn. [online]. 2010, vol.20, n.5, pp.781-788.  Epub Sep 24, 2010. ISSN 0102-695X.

A simple, reproducible and efficient high-performance liquid chromatography (HPLC) method was developed. Water (0.05% TFA):acetonitrile (0.05% TFA) was used as the mobile phase in a gradient system for the determination of epicatechin (EP) in leaves of Maytenus ilicifolia (Schrad.) Planch. The analysis was performed using an RP C-18 column (5 µm) as the stationary phase, with a flow rate of 0.8 mL/min, at a wavelength of 210 nm for detection and determination. The main validation parameters of the method were also determined. The calibration curve was found to be linear, with a range of 10-120 µg/mL (EP). The correlation coefficient of the linear regression analysis was within 0.9988, and the detection and quantification limits were 28.61 and 86.77 µg/mL, respectively. The content of EP was successfully determined, with satisfactory reproducibility and recovery. Recovery of the EP was 99.32%. The method was successfully applied to the determination of epicatechin in leaves of M. ilicifolia. The interlaboratorial evaluation showed the reproducibility of the method with a relative standard deviation of 14.62%.

Keywords : Maytenus ilicifolia; HPLC; epicatechin; analytical method development; quality control.

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