SciELO - Scientific Electronic Library Online

vol.13 issue3Purification and functional characterization of two fibrinogenolytic enzymes from Bothrops alternatus venomHistological and biochemical effects induced by sublethal doses of Bothrops jararacussu venom in mice author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Journal of Venomous Animals and Toxins including Tropical Diseases

On-line version ISSN 1678-9199


MULI, E. M.  and  MAINGI, J. M.. Antibacterial activity of Apis mellifera L. propolis collected in three regions of Kenya. J. Venom. Anim. Toxins incl. Trop. Dis [online]. 2007, vol.13, n.3, pp.655-663. ISSN 1678-9199.

The present study aimed at investigating the susceptibility of the microorganisms Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis to ethanolic extracts of propolis (EEP) from three regions of Kenya (Taita, Tana and Samburu). Propolis was extracted using four different concentrations of ethanol: pure, 70%, 50%, and 30%. Ethanol (70%) and Streptomycin were used as controls. The agar diffusion method using filter paper disks was employed. Antibacterial activity was determined as an equivalent of the inhibition zones diameters (in millimeters) after incubation at 37°C for 24h. Significant differences in the antibacterial activities of propolis were observed among the three regions, depending on the test microorganisms and on the procedure used for the preparation of propolis extract. Bacillus subtilis and Staphylococcus aureus were the most susceptible bacteria and 70% EEP had the best antibacterial effect.

Keywords : Apis mellifera; propolis; antibacterial agents; disk diffusion antimicrobial tests.

        · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License