SciELO - Scientific Electronic Library Online

 
vol.87 issue1Does ethnic ancestry play a role in smoking?Gestational diabetes: a risk of puerperal hypovitaminosis A? author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Anais da Academia Brasileira de Ciências

Print version ISSN 0001-3765

Abstract

DREYFUSS, JULIANA L. et al. Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis. An. Acad. Bras. Ciênc. [online]. 2015, vol.87, n.1, pp.455-462.  Epub Feb 24, 2015. ISSN 0001-3765.  https://doi.org/10.1590/0001-3765201520140122.

The adenoviral conjunctivitis is one of the biggest causes of conjunctival infection in the world. Conjunctivitis causes relatively nonspecific symptoms, as hyperaemia and chemosis. Even after biomicroscopy, complex laboratory tests, such as viral culture, are necessary to identify the pathogen or its etiology. To contribute to the better understanding of the pathobiology of the adenoviral conjunctivitis, the tear fluids of patients with unilateral acute adenovirus conjunctivitis (UAAC), normal donors (control) and patients with allergic conjunctivitis were analyzed. Tear samples were collected with Schirmer strips from control, allergic conjunctivitis and UAAC patients, diagnosed by clinical signs. UAAC tears were tested positive in viral cultures. After the elution, HA was quantified using an ELISA-like fluorometric assay and the protein profile was determined by SDS-PAGE. A profound increase in the HA tear content in UAAC patients was found when compared to control and ALC. This HA increase in UAAC tears remarkably was not observed in tears from contralateral eyes without clinical signs, nor in allergic conjunctivitis. In addition a distinct profile of UAAC tear proteins was observed in patients with UAAC. The quantification of HA in the tear fluid is a rapid, sensitive and specific test. This molecule might be a biomarker candidate for acute conjunctivitis.

Keywords : conjunctivitis; glycosaminoglycans; hyaluronic acid; tear film.

        · abstract in Portuguese     · text in English     · English ( pdf )