SciELO - Scientific Electronic Library Online

vol.78 issue3Association between statin use and mortality risks during the acute phase of ischemic stroke in patients admitted to an intensive care unitNeurology, psychiatry and the chess game: a narrative review author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Arquivos de Neuro-Psiquiatria

Print version ISSN 0004-282XOn-line version ISSN 1678-4227


DA SILVA, Samya Jezine et al. Cerebrospinal fluid challenges for the diagnosis of herpes simplex infection in the central nervous system. Arq. Neuro-Psiquiatr. [online]. 2020, vol.78, n.3, pp.163-168.  Epub Mar 16, 2020. ISSN 1678-4227.

Herpes simplex virus (HSV) is a cause of a severe disease of the central nervous system (CNS) in humans. The demonstration of specific antibodies in the cerebrospinal fluid (CSF) may contribute to the retrospective neurological diagnosis. However, the commercial immunological tests for HSV infection are for use in serum samples.


The aim of the present study was to adapt a commercial kit anti-HSV IgG used for serum samples to be performed with a CSF sample.


Forty CSF specimens from 38 patients with suspected CNS HSV infection were serially diluted for detecting anti-HSV IgG by enzyme immunoassay (EIA). The same samples were also analyzed with the polymerase chain reaction (PCR).


The sensitivity of EIA test for HSV was 5% (dilution 1:40) and 65% (dilution 1:2) in CSF, and HSV DNA PCR was 15%. The combined analysis of EIA (dilution 1:2) and PCR increased the sensitivity up to 72.5%. The inflammatory CSF was associated with positive HSV PCR.


We demonstrated the importance to adapt serological anti-HSV IgG EIA test for CSF assays to increase the accuracy of the analysis, considering the low concentration of specific antibodies in CSF.

Keywords : herpes simplex virus; cerebrospinal fluid; enzyme immunoassay; polymerase chain reaction.

        · abstract in Portuguese     · text in English     · English ( pdf )