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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946


GARCIA, Nilma Maciel; ASSIS, Cezar Mendes de; DEL NEGRO, Gilda Maria Barbaro  and  LACAZ, Carlos da Silva. Histoplasma capsulatum exocellular antigens. Obtention in neopeptone, glucose, thiamine and asparagine medium (NGTA). Rev. Inst. Med. trop. S. Paulo [online]. 1990, vol.32, n.5, pp.370-374. ISSN 1678-9946.

The purpose of this work is obtaining exocellular antigens H and M from 4 H. capsulatum strains using NGTA medium (neopeptone, glucose, thiamine and asparagine) for periods of 1,2 and 3 months, at 36ºC and continuously shaken. The exocellular antigens were evaluated by double immunodiffusion test against H. capsulatum rabbit antiserum, 7 histoplasmosis sera, 4 paracoccidioidomycosis sera and a reference antigen and antibody furnished by C.D.C. (Atlanta - USA). Except for the exocellular antigen from strain B.679 with 1 month of culture, all exocellular antigens obtained from the strains B.679, 58 and O187 showed the H and M bands. The A.811 strain demonstrated only the fraction H. All the exocellular antigens reacted positively with sera from histoplasmosis patients, except those obtained from strains 58 and B.679 with 1 month of culture. With regard to paracoc-cidioidomycosis patients sera, the exocellular antigens from strains 58 and O187 did not cross-react with them.

Keywords : Histoplasma capsulatum; Imunodifusão dupla; Exoantígenos H e M; Meio de NGTA.

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