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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Abstract

VIDAL, Monica Scarpelli Martinelli et al. Paracoccidioides brasiliensis: a mycologic and immunochemical study of a sample isolated from an armadillo (Dasipus novencinctus). Rev. Inst. Med. trop. S. Paulo [online]. 1995, vol.37, n.1, pp.43-49. ISSN 1678-9946.  https://doi.org/10.1590/S0036-46651995000100007.

A sample of P. brasiliensis isolated from the spleen and the liver of an armadillo (Dasipus novencinctus) has been analysed under a mycological and immunochemical viewpoint. The armadillo was captured in an area of Tucuruí (State of Pará, Brazil), the animal being already established as an enzootic reservoir of P. brasiliensis at that region of the country. This sample maintained in the fungal collection of the Tropical Medicine Institute of São Paulo (Brazil) numbered 135, has got all the characteristics of P. brasiliensis, with a strong antigenic power and low virulence for guinea-pigs and Wistar rats. The specific exoantigen of P. brasiliensis - the glycoprotein with a molecular weight of 43 kDa - was easily demonstrated with double immunodiffusion, immunoelectrophoresis, SDS-PAGE and immunobloting techniques.

Keywords : Paracoccidioides brasiliensis; Dasipus novencinctus.

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