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Revista da Sociedade Brasileira de Medicina Tropical

Print version ISSN 0037-8682On-line version ISSN 1678-9849

Abstract

GHANAVATI, Roya et al. Clonal relation and antimicrobial resistance pattern of extended-spectrum β-lactamase- and AmpC β-lactamase-producing Enterobacter spp. isolated from different clinical samples in Tehran, Iran. Rev. Soc. Bras. Med. Trop. [online]. 2018, vol.51, n.1, pp.88-93. ISSN 1678-9849.  https://doi.org/10.1590/0037-8682-0227-2017.

INTRODUCTION:

Here, we determined the genes encoding antibiotic resistance enzymes and virulence factors and evaluated the genetic relationship between Enterobacter spp. isolated from different clinical samples.

METHODS:

A total of 57 clinical isolates of Enterobacter spp. were tested for the production of extended-spectrum β-lactamases (ESBLs), carbapenemase, and AmpC using phenotypic and genotypic methods.

RESULTS:

The most common ESBLs and AmpC β-lactamases were bla TEM (63.3%) and bla EBC (57.7%), respectively. The most prevalent virulence gene was rpos (87.7%). The random amplified polymorphic DNA (RAPD) patterns of strains were genetically unrelated.

CONCLUSIONS:

RAPD polymerase chain reaction analysis revealed high genetic diversity among isolates.

Keywords : Enterobacter; ESBL; AmpC; RAPD-PCR.

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