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Pesquisa Agropecuária Brasileira

Print version ISSN 0100-204XOn-line version ISSN 1678-3921


CALIXTO, Marcia Cristina; MOURAO FILHO, Francisco de Assis Alves; MENDES, Beatriz Madalena Januzzi  and  VIEIRA, Maria Lúcia Carneiro. Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck. Pesq. agropec. bras. [online]. 2004, vol.39, n.7, pp.721-724. ISSN 1678-3921.

This work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of 'Hamlin' sweet orange + 'Indian Red' pummelo and 'Hamlin' sweet orange + 'Singapura' pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs.

Keywords : genetic improvement; protoplast fusion; rootstock.

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