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Pesquisa Veterinária Brasileira

versão impressa ISSN 0100-736Xversão On-line ISSN 1678-5150

Resumo

TEIXEIRA, Marjorie F.B. et al. A monoclonal blocking ELISA for the serological diagnosis of bovine herpesvirus type 1 (BHV-1) infections. Pesq. Vet. Bras. [online]. 2001, vol.21, n.1, pp.33-37. ISSN 1678-5150.  https://doi.org/10.1590/S0100-736X2001000100007.

A monoclonal antibody-based blocking enzyme linked immunosorbent assay (ELISA-M) was developed and standardized for the detection of antibodies to infectious bovine rhinotracheitis virus (Bovine Herpesvirus type 1; BHV-1). A total of 266 samples of bovine sera (148 negative and 118 positive) were tested and compared with the results of a standard serum neutralization (SN) test. The ELISA-M was adjusted to 92.37% sensitivity, 92.56% especificity, 93.83% negative predictive value, 90.83% positive predictive value and to an accuracy of 92.48%, with an agreement index (k) equal to 0.85. The main advantages presented by the ELISA-M were its practicality and rapidity in performance. This test was shown to be a suitable alternative to SN tests in the detection of BHV-1 antibodies in cattle. However, the ELISA was unable to discriminate between BHV-1 and bovine herpesvirus type 5 (BHV-5) antibodies.

Palavras-chave : Bovine herpesvirus; BHV-1; infectious bovine rhinotracheitis virus; ELISA; monoclonal.

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