SciELO - Scientific Electronic Library Online

 
vol.52 número4Differential expression of microRNA-411 and 376c is associated with hypertension in pregnancyAltered peripheral lymphocyte subsets in untreated systemic lupus erythematosus patients with infections índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Brazilian Journal of Medical and Biological Research

versão impressa ISSN 0100-879Xversão On-line ISSN 1414-431X

Resumo

ZHANG, You En et al. Hydrogen sulfide protects H9c2 cardiomyoblasts against H2O2-induced apoptosis. Braz J Med Biol Res [online]. 2019, vol.52, n.4, e7626.  Epub 15-Abr-2019. ISSN 1414-431X.  https://doi.org/10.1590/1414-431x20187626.

Reactive oxygen species (ROS) are highly reactive chemical species that may cause irreversible tissue damage, and play a critical role in cardiovascular diseases. Hydrogen sulfide (H2S) is a gasotransmitter that acts as a ROS scavenger with cardio-protective effects. In this study, we investigated the cytoprotective effect of H2S against H2O2-induced apoptosis in cardiomyocytes. H9c2 rat cardiomyoblasts were treated with H2S (100 μM) 24 h before challenging with H2O2 (100 μM). Apoptosis was then assessed by annexin V and PI, and mitochondrial membrane potential was measured using a fluorescent probe, JC-1. Our results revealed that H2S improved cell viability, reduced the apoptotic rate, and preserved mitochondrial membrane potential. An increased Bcl-2 to Bax ratio was also seen in myocytes treated with H2S after H2O2-induced stress. Our findings indicated a therapeutic potential for H2S in preventing myocyte death following ischemia/reperfusion.

Palavras-chave : Reactive oxygen species; ROS; Hydrogen sulfide; H2O2-induced apoptosis; Cardiomyocytes.

        · texto em Inglês     · Inglês ( pdf )