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Acta Botanica Brasilica

Print version ISSN 0102-3306

Abstract

DORNELLES, Liane T.  and  PETERS, José Antônio. Regeneration of rice plants (Oryza sativa L.) from immature panicles. Acta Bot. Bras. [online]. 1992, vol.6, n.2, pp.97-104. ISSN 0102-3306.  https://doi.org/10.1590/S0102-33061992000200006.

Aiming to observe the direct and indirect regeneration potencial, immature panicles of rice cultivars BR IRGA 410 and 414 were cultured on Murashige & Skoog's basal medium complemented with either lmg/l or 2 mg/l 2,4-D or 2 mg/12,4-D or 2 mg/l 2,4-D + 0,2 mg/l K for callus induction and with 1 mg/l of ANA + 4 mg/l of K for direct bud regeneration. In both genotypes, 100% of the panicles developed callus, but the regeneration showed dependency on the callus forming medium. Throguh direct regeneration, 100% of the panicles from both cultivars developed buds with average of 5.8 buds/panicle. ABREVIATION: Murashige, and Skoog medium; 2,4-D - ácido 2,4 doclorofenoxiacetic acid; K - Kinetin; AIA - indole - 3 - acetic acid; ANA - acido - naphthaleneacetic acid.

Keywords : Oryza sativa L; regeneration; immature panicles.

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