SciELO - Scientific Electronic Library Online

 
vol.19 issue1LIQUID-LIQUID EQUILIBRIA OF THE ACETIC ACID-WATER-MIXED SOLVENT (CYCLOHEXYL ACETATE-CYCLOHEXANOL) SYSTEMANALYSIS OF TRICKLE BED AND PACKED BUBBLE COLUMN BIOREACTORS FOR COMBINED CARBON OXIDATION AND NITRIFICATION author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Brazilian Journal of Chemical Engineering

Print version ISSN 0104-6632On-line version ISSN 1678-4383

Abstract

ZANGIROLAMI, T.C.; CARLSEN, M.; NIELSEN, J.  and  J0RGENSEN, S.B.. GROWTH AND ENZYME PRODUCTION DURING CONTINUOUS CULTURES OF A HIGH AMYLASE-PRODUCING VARIANT OF Aspergillus Oryzae. Braz. J. Chem. Eng. [online]. 2002, vol.19, n.1, pp.55-68. ISSN 1678-4383.  http://dx.doi.org/10.1590/S0104-66322002000100005.

Growth and product formation by a selected variant of Aspergillus oryzae showing high a-amylase production was studied in continuous cultivations carried out at six different specific growth rates, using glucose as the growth-limiting nutrient. The analysis of the steady-state data revealed that the variant and wild-type strains were similar with respect to glucose uptake system and stoichiometric coefficients. However, the variant was capable of maintaining an enzyme production as high as 40 FAUgDW-1h-1 at a dilution rate of 0.2 h-1, while the wild-type strain reached a maximum specific a-amylase production rate of 17 FAUgDW-1h-1 at a dilution rate of 0.1 h-1. Using a morphologically structured model originally proposed for the wild-type strain, it was possible to describe enzyme production, biomass formation and glucose consumption after modification of a few parameters to adjust the model to the characteristics of the selected variant.

Keywords : Aspergillus oryzae; a-amylase; continuous cultivations; morphologically structured model.

        · text in English

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License