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vol.26 número2NEGATIVE EFFECTS ON PHOTOSYNTHESIS AND CHLOROPLAST PIGMENTS EXPOSED TO LEAD AND ALUMINUM: A META-ANALYSISWOOD RESOURCE BALANCE FOR PLANTATION FORESTS IN BRAZIL: RESOURCES, CONSUMPTION AND CASCADING USE índice de autoresíndice de assuntospesquisa de artigos
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CERNE

versão impressa ISSN 0104-7760versão On-line ISSN 2317-6342

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SANTOS, Maricélia Moreira dos et al. DISINFECTION PROTOCOL AND IN VITRO GERMINATION OF SEEDS OF Dalbergia nigra. CERNE [online]. 2020, vol.26, n.2, pp.238-246.  Epub 30-Set-2020. ISSN 2317-6342.  https://doi.org/10.1590/01047760202026022714.

The Atlantic Forest is a biome that has suffered anthropic actions, such as the extraction of hardwood, which can lead to the extinction of endemic species of great economic and ecological value, such as Dalbergia nigra. In this perspective, large-scale multiplication studies are necessary to contribute to the conservation of this species. The objective of this work was to establish a protocol for the in vitro production of axenic seedlings of D. nigra. Four experiments were performed: I. Immersion times in NaOCl. II. Disinfecting agents in seed germination of D. nigra. III. NaOCl toxicity in seeds of the model species Lactuca sativa L. IV. Culture media and glutamine in the germination and initial growth of D. nigra. All experiments were arranged in a completely randomized design. For a better disinfestation and less harmful effects to the seedlings, it is recommended that the seeds be treated with 70% alcohol (1 min) and NaOCl (14 min). NaOCl has a high phytotoxic, cytotoxic, and genotoxic effect, and its mechanism of action in the L. sativa cell cycle is clastogenic and aneugenic, suggesting the formation of abnormal seedlings in D. nigra. The WPM medium and its supplementation with glutamine (0.75 mg.L-1) are recommended.

Palavras-chave : Bahia rosewood; In vitro cultivation; Disinfecting agentes; Sodium hypochlorite toxicity; Culture media; Glutamine.

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