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vol.38 issue3Lecirelin and Buserelin (Gonadotrophin releasing hormone agonists) are equally effective for fixed time insemination in buffalo author indexsubject indexarticles search
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Brazilian Journal of Veterinary Research and Animal Science

Print version ISSN 1413-9596On-line version ISSN 1678-4456


CORTEZ, Adriana et al. Comparison of a commercial enzyme immune assay (ELISA) and serum neutralization techniques for the detection of antibodies against BHV-1 in buffaloes (Bubalus bubalis) sera. Braz. J. Vet. Res. Anim. Sci. [online]. 2001, vol.38, n.3, pp.146-148. ISSN 1678-4456.

The performance of a commercial ELISA (Bovine Rhinotracheitis virus antibody test Kit, Herdcheck®, IDEXX Laboratories Inc., USA) designed to detect the presence of antibody to the Bovine Herpesvirus type 1 (BHV-1) in bovine serum, was evaluated in samples of buffaloes (Bubalus bubalis) serum. Sera of 133 buffaloes without IBR vaccination were performed both by ELISA and standard serum neutralization technique. Sensitivity and specificity of ELISA were 97.14% and 46.03%, respectively. The estimated concordance between two serologic diagnostic methods by kappa index was 0.44 IC (0.29-0.59) using 95% confidence level.

Keywords : Infections bovine rhinotracheites; ELISA; Buffaloes; Bubalus bubalis.

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