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Genetics and Molecular Biology

Print version ISSN 1415-4757On-line version ISSN 1678-4685

Abstract

BRIEGER, Jürgen; WEIDT, Eberhard J.  and  DECKER, Jochen. Fast, non-toxic, and inexpensive n-butanol preparation of recombinant plasmids. Genet. Mol. Biol. [online]. 2000, vol.23, n.2, pp.299-300. ISSN 1678-4685.  http://dx.doi.org/10.1590/S1415-47572000000200010.

Various commercial and non-commercial plasmid preparation protocols are currently available. However, the kits are expensive and many of the protocols contain toxic chemicals. Here we present a novel, optimized and, therefore, very advantageous plasmid preparation protocol using n-butanol. The preparation can be performed quickly and no toxic chemicals are used, at overall costs of about one cent per plasmid preparation.

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