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Genetics and Molecular Biology

Print version ISSN 1415-4757On-line version ISSN 1678-4685


JAVIER TOVAR, Francisco; SILVA, Luiz Antônio Ferreira da; RODARTE, Renato Santos  and  LEONCINI, Orilio. Characterization of ribosomal DNA (rDNA) in Drosophila arizonae. Genet. Mol. Biol. [online]. 2000, vol.23, n.2, pp.231-233. ISSN 1678-4685.

Ribosomal DNA (rDNA) is a multigenic family composed of one or more clusters of repeating units (RU). Each unit consists of highly conserved sequences codifying 18S, 5.8S and 28S rRNA genes intercalated with poorly conserved regulatory sequences between species. In this work, we analyzed the rDNA of Drosophila arizonae, a member of the mulleri complex (Repleta group). Using genomic restriction patterns, cloning and mapping of some representative rDNA fragments, we were able to construct a representative restriction map. RU in this species are 13.5-14 kb long, restriction sites are completely conserved compared with other drosophilids and the rDNA has an R1 retrotransposable element in some RU. We were unable to detect R2 elements in this species.

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