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Genetics and Molecular Biology

Print version ISSN 1415-4757On-line version ISSN 1678-4685


BARROS, Everaldo G. de; TINGEY, Scott  and  RAFALSKI, J. Antoni. Sequence characterization of hypervariable regions in the soybean genome: leucine-rich repeats and simple sequence repeats. Genet. Mol. Biol. [online]. 2000, vol.23, n.2, pp.411-415. ISSN 1678-4685.

The genetic basis of cultivated soybean is rather narrow. This observation has been confirmed by analysis of agronomic traits among different genotypes, and more recently by the use of molecular markers. During the construction of an RFLP soybean map (Glycine soja x Glycine max) the two progenitors were analyzed with over 2,000 probes, of which 25% were polymorphic. Among the probes that revealed polymorphisms, a small proportion, about 0.5%, hybridized to regions that were highly polymorphic. Here we report the sequencing and analysis of five of these probes. Three of the five contain segments that encode leucine-rich repeat (LRR) sequence homologous to known disease resistance genes in plants. Two other probes are relatively AT-rich and contain segments of (A)n/(T)n. DNA segments corresponding to one of the probes (A45-10) were amplified from nine soybean genotypes. Partial sequencing of these amplicons suggests that deletions and/or insertions are responsible for the extensive polymorphism observed. We propose that genes encoding LRR proteins and simple sequence repeat region prone to slippage are some of the most hypervariable regions of the soybean genome.

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