SciELO - Scientific Electronic Library Online

 
vol.37 número4Identification of novel and conserved microRNAs in Coffea canephora and Coffea arabicaClustering of water bodies in unpolluted and polluted environments based on Escherichia coli phylogroup abundance using a simple interaction database índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Genetics and Molecular Biology

versão impressa ISSN 1415-4757

Resumo

CEITA, Geruza de Oliveira et al. Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosa. Genet. Mol. Biol. [online]. 2014, vol.37, n.4, pp.683-693.  Epub 03-Nov-2014. ISSN 1415-4757.  https://doi.org/10.1590/S1415-47572014005000017.

The phytopathogenic fungus Moniliophthora perniciosa (Stahel) Aime & Philips-Mora, causal agent of witches' broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and virulence. In this study, sequences deposited in the M. perniciosa Genome Sequencing Project database were analyzed to identify potential biological targets. For the first time, the ergosterol biosynthetic pathway in M. perniciosa was studied and the lanosterol 14α-demethylase gene (ERG11) that encodes the main enzyme of this pathway and is a target for fungicides was cloned, characterized molecularly and its phylogeny analyzed. ERG11 genomic DNA and cDNA were characterized and sequence analysis of the ERG11 protein identified highly conserved domains typical of this enzyme, such as SRS1, SRS4, EXXR and the heme-binding region (HBR). Comparison of the protein sequences and phylogenetic analysis revealed that the M. perniciosa enzyme was most closely related to that of Coprinopsis cinerea.

Palavras-chave : Basidiomycota; fungus; ergosterol; Theobroma cacao.

        · texto em Inglês     · Inglês ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons