SciELO - Scientific Electronic Library Online

vol.42 número3Mapping of QTL for total spikelet number per spike on chromosome 2D in wheat using a high-density genetic mapAmmopiptanthus mongolicus stress-responsive NAC gene enhances the tolerance of transgenic Arabidopsis thaliana to drought and cold stresses índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados




Links relacionados


Genetics and Molecular Biology

versão impressa ISSN 1415-4757versão On-line ISSN 1678-4685


LI, Wei et al. Genome-wide identification and characterization of R2R3-MYB genes in Medicago truncatula. Genet. Mol. Biol. [online]. 2019, vol.42, n.3, pp.611-623.  Epub 14-Nov-2019. ISSN 1678-4685.

MYB is a large family of plant transcription factors. Its function has been identified in several plants, while there are few reports in Medicago truncatula. In this study, we used RNA-seq data to analyze and identify R2R3-MYB genes in the genome of Medicago truncatula. Phylogenetic analysis classified 150 MtMYB genes into 21 subfamilies with homologs. Out of the 150 MtMYB genes, 139 were distributed among 8 chromosomes, with tandem duplications (TD) and segment duplications (SD). Microarray data were used for functional analysis of the MtMYB genes during growth and developmental processes providing evidence for a role in tissues differentiation, seed development processes, and especially the nodulation process. Furthermore, we investigated the expression of MtMYB genes in response to abiotic stresses using RNA-seq data, which confirmed the critical roles in signal transduction and regulation processes under abiotic stress. We used quantitative real-time PCR (qRT-PCR) to validate expression profiles. The expression pattern of M. truncatula MYB genes under different abiotic stress conditions suggest that some may play a major role in cross-talk among different signal transduction pathways in response to abiotic stresses. Our study will serve as a foundation for future research into the molecular function of M. truncatula R2R3-MYB genes.

Palavras-chave : Medicago truncatula; MYB; abiotic stress; qRT-PCR.

        · texto em Inglês     · Inglês ( pdf )