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Brazilian Archives of Biology and Technology

Print version ISSN 1516-8913On-line version ISSN 1678-4324


PELOZO, Maria Inez de Godoy; CARDOSO, Mara Lane Carvalho  and  MELLO, João Carlos Palazzo de. Spectrophotometric determination of tannins and caffeine in preparations from Paullinia cupana var. sorbilis. Braz. arch. biol. technol. [online]. 2008, vol.51, n.3, pp.447-451. ISSN 1678-4324.

The present work was designed to quantify the caffeine and total polyphenols in the extractive solution and the granulated form from the seeds of Paullinia cupana var. sorbilis, by the spectrophotometric method. The method showed linearity for the caffeine and polyphenols in the range of 5-25 µg/ml and 2.4-5.6 µg/ml respectively. The solutions of the semipurified fraction (EPA) and granulated form (GRA) showed linear responses in the range of 0.288-0.672 and 0.4-1.2 µg/ml, respectively. The precision and accuracy were determined for the EPA solution at a concentration of 100 µg/ml. The spectrophotometric method performed well in quantifying the caffeine and total polyphenols.

Keywords : Caffeine; Paullinia cupana; Total polyphenols; Spectrophotometric quantification assay.

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