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Brazilian Journal of Microbiology

Print version ISSN 1517-8382

Abstract

SMANIOTTO, Alessandra et al. 'Synthetic lipase' production from a newly isolated Sporidiobolus pararoseus strain by submerged fermentation. Braz. J. Microbiol. [online]. 2012, vol.43, n.4, pp.1490-1498. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822012000400033.

The lipase produced by a newly isolate Sporidiobolus pararoseus strain has potential catalysis ability for esterification reactions. In order to improve its synthetic activity, this work aimed at optimizing 'synthetic lipase' production by submerged fermentation of a conventional media based on peptone, yeast extract, NaCl and olive oil using experimental design technique. According to the results obtained in the first experimental design (24-1), yeast extract and NaCl concentrations were tested to further optimization by response surface methodology. The maximum 'synthetic lipase' activity obtained was 26.9 U/mL in the optimized media (5.0, 6.8, 7.0 and 1.0% (wt/v) of peptone, yeast extract, NaCl and olive oil, respectively), representing a 6.36-fold increase compared to the initial medium. The time course of 'synthetic lipase' production in the optimized condition was evaluated in terms of synthetic activity, protease activity, biomass and total carbon and the maximum synthetic activity was observed during the stationary phase of growth.

Keywords : Sporidiobolus pararoseus; experimental design; 'synthetic lipase'; submerged fermentation.

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