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Brazilian Journal of Microbiology

Print version ISSN 1517-8382

Abstract

SMANIOTTO, Alessandra et al. Concentration, characterization and application of lipases from Sporidiobolus pararoseus strain. Braz. J. Microbiol. [online]. 2014, vol.45, n.1, pp.294-301. ISSN 1517-8382.  https://doi.org/10.1590/S1517-83822014000100043.

Lipases produced by a newly isolated Sporidiobolus pararoseus strain have potential catalytic ability for esterification reactions. After production, the enzymatic extracts (conventional crude and precipitated, 'CC' and 'CP', and industrial crude and precipitated, 'IC' e 'IP') were partially characterized. The enzymes presented, in general, higher specificity for short chain alcohols and fatty acids. The precipitated extract showed a good thermal stability, higher than that for crude enzymatic extracts. The 'CC' and 'CP' enzymes presented high activities after exposure to pH 6.5 and 40 ºC. On the other hand, the 'IC' and 'IP' extracts kept their activities in a wide range of pH memory but presented preference for higher reaction temperatures. Preliminary studies of application of the crude lipase extract in the enzymatic production of geranyl propionate using geraniol and propionic acid as substrates in solvent-free system led to a reaction conversion of 42 ± 1.5%.

Keywords : Sporidiobolus pararoseus; 'synthetic lipase'; submerged fermentation; enzyme characterization.

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