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Acta Scientiarum. Agronomy

Print version ISSN 1679-9275On-line version ISSN 1807-8621


OLIVEIRA-LONGATTI, Silvia Maria de; MARRA, Leandro Marciano; CARVALHO, Teotonio Soares de  and  MOREIRA, Fatima Maria de Souza. The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria. Acta Sci., Agron. [online]. 2020, vol.42, e44332.  Epub July 03, 2020. ISSN 1807-8621.

Agar media with insoluble phosphates are used for semi-quantitative screening of phosphate-solubilizing bacteria based on the solubilization halo formed around the colonies. We show that the volume of the culture medium (15, 20, and 30 mL) and the inoculation method (toothpick vs microdrop) strongly influence the diameter of the solubilization halo, and this should be considered in advance selection of the isolates most efficient in this process.

Keywords : insoluble phosphate; plant growth-promoting rhizobacteria; solubilization halo; microdrop; toothpick.

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