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vol.29 issue1EFFECT OF NITROGEN-FIXING BACTERIA ON GRAIN YIELD AND DEVELOPMENT OF FLOODED IRRIGATED RICEQUALITY OF MINIMALLY PROCESSED YAM (Dioscorea sp.) STORED AT TWO DIFFERENT TEMPERATURES author indexsubject indexarticles search
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Revista Caatinga

Print version ISSN 0100-316XOn-line version ISSN 1983-2125

Abstract

COLOMBO, RONAN CARLOS et al. RESPONSE OF Cattleya forbesii ORCHID TO INCREASING SILICON CONCENTRATIONS IN VITRO. Rev. Caatinga [online]. 2016, vol.29, n.1, pp.18-24. ISSN 1983-2125.  https://doi.org/10.1590/1983-21252016v29n103rc.

Addition of Silicon (Si) to culture media has been shown to improve the development of seedlings grown in vitro, and to reduce losses during the acclimatization phase. The objective of this study was to evaluate the in vitro growth of Cattleya forbesii (Orchidaceae) in MS medium containing five different concentrations of SiO2 (0.0, 0.5, 1.0, 1.5, and 2.0 g·L−1). At day 200, the following variables were measured: number of roots, average length of the root system, leaf area, number of leaves and shoots, shoot height, fresh and dry masses of roots and shoots, water content of roots and shoots, and pH of the culture medium. Most variables decreased as the concentration of Si increased, reducing the in vitro vegetative growth of C. forbesii. Accumulation of Si in leaf tissues was detected by scanning electron microscopy, confirming uptake by plants. The Si source and concentrations tested showed no beneficial effect on in vitro growth of C. forbesii.

Keywords : Micropropagation; Culture medium; Amorphous silica..

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