SciELO - Scientific Electronic Library Online

vol.17 issue4A genetic study of traits related to canopy coverage in common beanSelection of energy cane clones author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Crop Breeding and Applied Biotechnology

Print version ISSN 1518-7853On-line version ISSN 1984-7033


SILVA, Luis Felipe Lima e et al. Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content. Crop Breed. Appl. Biotechnol. [online]. 2017, vol.17, n.4, pp.320-326. ISSN 1984-7033.

The aim of this study was to perform chromosome counts and nuclear DNA quantification of the unconventional vegetables species: bertalha (Basella alba L.), vinagreira verde (Hibiscus sabdariffa L.), azedinha (Rumex acetosa L.), peixinho (Stachys byzantina K. Koch), and capuchinha (Tropaeolum majus L.). Metaphase chromosomes were obtained from the pre-treatment of root meristem with 8-hydroxyquinoline or colchicine and lides were prepared by the flame-drying technique and stained with 5% Giemsa. DNA quantification was performed by flow cytometry. Chromosome number and DNA content (pg) estimated for each species were: Rumex acetosa: 2n=2x=14 and 7.04 pg; Basella alba: 2n=2x=44 and 7.05 pg; Tropaeolum majus: 2n=2x=28 and 2.08 pg; Stachys byzantine: 2n=2x=30 and 1.54 pg, and Hibiscus sabdariffa: 2n=4x=72 and 5.12 pg.

Keywords : Underutilized vegetables; germplasm; plant breeding; cytogenetic analysis.

        · text in English     · English ( pdf )