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Arquivos de Neuro-Psiquiatria

Print version ISSN 0004-282X

Arq. Neuro-Psiquiatr. vol.28 no.2 São Paulo June 1970 

Depletion of biogenic amines and enhancement of cholinergic activity in the olfactory bulb and central olfactory connections with chronic methedrine intoxication


Depleção de aminas biogênicas e aumento da atividade colinérgica no bulbo olfatorio e nas conexões olfatórias centrais mediante intoxicação pela metedrina



O. Duarte-Escalante; E. Ellinwood Jr.

Department of Surgery and Psychiatry, Duke University Medical Center, Durham, North Carolina





Em seqüência a estudos anteriores os autores visam, neste relato, a apresentar as alterações histoquímicas que ocorrem no sistema olfatório de gatos nos quais se desenvolveu nítida estereotipia de fungação (sniffing) após administração prolongada de metedrina. Foram intoxicados 12 gatos mediante injeções diárias, durante 10 dias, de doses progressivas de metedrina. Os tecidos a examinar (bulbos olfatórios e suas conexões centrais) foram preparados histoquimicamente para demonstrar a fluorescência das aminas biogênicas e reações colinérgicas. Mediante algumas modificações à metodologia recomendada por outros pesquisadores, os autores puderam demonstrar a presença de monoaminas e de acetilcolina no bulbo olfatório e de grupos de fibras adrenérgicas curtas e multi-ramificadas que parecem ser conectadas com os neurônios fluorescentes do bulbo olfatório, a partir de onde estabelecem conexões, pelo tracto olfatório medial, com os neurônios do septum e, pelo tracto olfatório lateral, com os neurônios do complexo amigdalóide.



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Duke University Medical Center — P. O. 2922 — Durham, North Carolina 27706 — U.S.A.
This study was supported in part by a Post-Doctoral Research Fellowship from the National Institutes of Mental Health, Grant No. 2F11 NB 2006-02 NSRA and Grant No. 1-R01-MH-15907-01 at the Department of Surgery and Psychiatry, Duke University Medical Center, Durham, North Carolina.
A part of this material was presented at the Ninth International Congress of Neurology, New York, September, 1969.
Author's acknowledgement. We are indebted to Miss Linda Nelson and Mrs. Donna Thompson for their technical assistances in this work. Thanks are also due to Mr. Don Powell for the preparation of the photographic material.

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