SciELO - Scientific Electronic Library Online

vol.60 issue3Genetic diversity in sugar apple (Annona squamosa L.) by using RAPD markersEffect of acetic acid on rice seeds coated with rice husk ash author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand



  • English (pdf)
  • Article in xml format
  • How to cite this article
  • SciELO Analytics
  • Curriculum ScienTI
  • Automatic translation


Related links


Revista Ceres

Print version ISSN 0034-737X

Rev. Ceres vol.60 no.3 Viçosa May/June 2013 



Rootstocks resistant to Meloidogyne incognita and compatibility of grafting in net melon


Porta-enxertos resistentes a Meloidogyne incognita e compatibilidade de enxertia de melão rendilhado



Francine de Souza GalattiI; Alexandre Junqueira FrancoI; Letícia Akemi ItoII; Hamilton de Oliveira CharloIII; Lucas Aparecido GaionI; Leila Trevisan BrazIV

IAgronomist Engineer. Departamento de Produção Vegetal, Universidade Estadual Paulista "Júlio de Mesquita Filho", Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Professor Paulo Donato Castellane, s/n, 14884-900, Jaboticabal, São Paulo, Brazil. (corresponding author);;
IIAgronomist Engineer, Master of Science. Departamento de Produção Vegetal, Universidade Estadual Paulista "Júlio de Mesquita Filho", Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Professor Paulo Donato Castellane, s/n, 14884-900, Jaboticabal, São Paulo, Brazil.
IIIAgronomist Engineer, Doctor of Science. Instituto Federal de Educação, Ciência e Tecnologia do Triângulo Mineiro, Rua João Batista Ribeiro, 4000, 38964-790, Uberaba, Minas Gerais, Brazil.
IVAgronomist Engineer, Doctor of Science. Departamento de Produção Vegetal, Universidade Estadual Paulista "Júlio de Mesquita Filho", Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Professor Paulo Donato Castellane, s/n, 14884-900, Jaboticabal, São Paulo, Brazil.




Due to the few studies about grafting in net melon, in order to obtain better control of soil pathogens, the aim of the present study was to evaluate 16 genotypes of Cucurbitaceae: Benincasa hispida, Luffa cylindrica, pumpkin 'Jacarezinho', pumpkin 'Menina Brasileira', squash 'Exposição', squash 'Coroa', pumpkin 'Canhão Seca', pumpkin 'Squash', pumpkin 'Enrrugado Verde', pumpkin 'Mini Paulista', pumpkin 'Goianinha', watermelon 'Charleston Gray', melon 'Rendondo Gaucho', melon 'Redondo Amarelo', cucumber 'Caipira HS' and cucumber 'Caipira Rubi', regarding to compatibility of grafting in net melon and resistance to Meloidogyne incognita, based on the reproduction factor (RF), according to Oostenbrink (1966). To assess resistance, the seedlings were transplanted to ceramic pots and inoculated with 300/mL eggs and/or second stage juveniles of M. incognita. At 50 days after transplanting, the plants were removed from the pots and the resistance was evaluated. The compatibility between resistant rootstock and grafts of net melon was determined by performing simple cleft grafting, in a commercial net melon hybrid of great market acceptance and susceptible to M. incognita (Bonus no. 2). The genotypes Luffa cylindrica, pumpkin 'Goianinha', pumpkin 'Mini-Paulista', melon 'Redondo Amarelo', watermelon 'Charleston Gray' are resistant to the nematode M. incognita. The better compatibilities occurred with the rootstocks melon 'Amarelo', which presented 100% of success, followed by pumpkin 'Mini-Paulista' with 94%. On the other hand, Sponge gourd, watermelon 'Charleston Gray' and pumpkin 'Goianinha' showed low graft take percentages of 66%, 62% and 50%, respectively.

Key words: Cucumis melo var. reticulatus, plant diseases, cucurbitaceae, nematode.


Devido aos poucos estudos realizados com enxertias em melão rendilhado, visando um maior controle de patógenos do solo, este trabalho teve por objetivo avaliar 16 genótipos de cucurbitáceas quanto à resistência a Meloidogyne incognita e a compatibilidade da enxertia do melão rendilhado. Foram avaliados 16 acessos de cucurbitáceas: Benincasa hispida, Bucha, Abóbora 'Jacarezinho', Abóbora 'Menina Brasileira', Moranga 'Exposição', Moranga 'Coroa', Abóbora 'Canhão Seca', Abóbora 'Squash', Mogango 'Enrrugado Verde', Abóbora 'Mini Paulista', Abóbora 'Goianinha', Melancia 'Charleston Gray', Melão 'Rendondo Gaúcho', Melão 'Redondo Amarelo', Pepino 'Caipira HS' e Pepino 'Caipira Rubi', quanto à resistência ao nematóide M. incognita, com base no fator de reprodução (FR), segundo Oostenbrink (1966). Para avaliação da resistência, as mudas foram transplantadas para vasos de cerâmica e foram aplicados 300 ovos ou juvenis de segundo estádio/mL de M. incognita, num total de 10 mL por vaso. Aos 50 dias após o transplantio, as plantas foram removidas dos vasos e realizou-se a avaliação da resistência. Para a compatibilidade entre os porta-enxertos resistentes e enxerto de melão rendilhado, foram realizadas enxertias do tipo garfagem fenda simples, em híbrido comercial de melão rendilhado de grande aceitação comercial e suscetíveil a M. incognita (Bônus N° 2). Os genótipos Bucha, Abóbora 'Goianinha', Abóbora 'Mini-Paulista', Melão 'Redondo Amarelo', Melancia 'Charleston Gray', foram resistentes ao nematóide M. incognita. As melhores compatibilidades ocorreram com os porta-enxertos Melão 'Amarelo', o qual teve 100% de pegamento, seguido da Abóbora 'Mini-Paulista' com 94%. Já Bucha, Melancia 'Charleston Gray' e Abóbora 'Goianinha', tiveram baixas porcentagens de pegamento: 66%, 62% e 50% respectivamente.

Palavras-chave: Cucumis melo var. reticulatus, doenças de plantas, cucurbitáceas, nematóides.




The net melon (Cucumis melo var. reticulatus Naud.) belongs to the botanical group Cantalupensis of the Cucurbitacea family, and it is characterized by the netting on the husk, round to oval shape and color of pulp varying between clear green and salmon (Rizzo & Braz, 2001). Unlike the others on the market, due to its appearance, aroma and higher level of soluble solids, this melon shows competitive advantages compared to other varieties, because it has a good market value and allows production in small areas with good yield (Factor et al., 2000).

Besides, light and relative humidity, temperature is the main climatic factor that affects melon crops, from the germination of the seeds, up to the final quality of the product (Costa et al., 2002), and for these conditions to be better controlled and to increase production, it is recommended to grow melons in a greenhouse.

According to Peil (2003), intensive growing of vegetables in greenhouse has caused serious problems with infestation by soil pathogens, such as root-knot nematodes, and salinization, which are increasingly difficult to be solved by traditional control methods. Therefore, grafting has become an alternative of necessary cultivation in contaminated areas, to prevent contact of the sensitive plant with the pathogenic agent.

Another problem which has limited the production of net melon under protected conditions is the incidence of nematodes of Meloidogyne group, which cause disruption of roots's cells resulting in galls and yellowing of leaves, leaves reduction, poor fruit quality and decrease of production. The gall nematodes also interact with bacteria and fungi causing complex diseases (Zitter et al, 1996). According to these authors, the environmentally safe and economic method of control is the use of resistant plants. Cucumis metuliferus is highly resistant to M. hapla, M. incognita, M. javanica and M. arenaria, but the development of hybrids with Cucumis spp has failed. Resistance to M. incognita and M. arenaria was identified in Cucumis anguria and others wild cucurbits.

Grafting of melons is little known and used in Brazil, due to the existence of not contaminated areas, but it is a technology utilized in many parts of the world, with the purpose of overcoming these problems (Martínez-Ballesta et al., 2010).

Grafting is a very effective practice for controling diseases caused by soil pathogens such as nematodes; this technique requires specialized procedures, high costs and longer times for seedlings to reach an ideal stage for transplanting. However, according to Goto et al. (2003), the cost-benefit ratio can make this technique feasible, and even reduce very high costs.

Therefore, the aim of the present study was to evaluate 16 genotypes of Cucurbitaceae regarding to resistance to Meloidogyne incognita and grafting compatibility of resistant rootstocks with net melon.



The experiments were carried out in a greenhouse at the School of Agricultural and Veterinary Sciences (FCAV-UNESP), Campus Jaboticabal.

Sixteen genotypes of Cucurbitaceae were evaluated: Benincasa hispida, Luffa cylindrica, pumpkin 'Jacarezinho', pumpkin 'Menina Brasileira', squash 'Exposição', squash 'Coroa', pumpkin 'Canhão Seca', pumpkin 'Squash', pumpkin 'Enrrugado Verde', pumpkin 'Mini Paulista', pumpkin 'Goianinha', watermelon 'Charleston Gray', melon 'Redondo Gaucho', melon 'Redondo Amarelo', cucumber 'Caipira HS' and cucumber 'Caipira Rubi' with regard to resistance to the nematode Meloidogyne incognita.

The seedlings were obtained by first seeding in Styrofoam trays, and 15 days after sowing, the seedlings were transplanted to pots. On the day of transplanting, 10 individual seedlings of each genotype were inoculated with eggs and/or second-stage juveniles of M. incognita, which consisted of the replicates. Inoculation was performed using a 10-mL graduated pipette to transfer the suspension of 300 eggs and/or second-stage juveniles / mL, henceforth referred to as the initial population (IP).

At 50 days after transplanting, the seedlings were removed from the pots, the aerial part discarded and the roots washed for the determination of the reproduction factor.

The resistance of the materials was defined based on the reproduction of the nematode in each genotype, in accordance with the concept of Roberts et al. (1998), where the resistance of a plant to a nematode is measured by the ability of the plant to suppress the development or reproduction of the pest. Thus, evaluation of the genotypes resistance to M. incognita was evaluated according to the reproduction factor (RF), as described by Oostenbrink (1966).

The population obtained for each root system, designated the final population (FP), was divided by the number of eggs and juveniles according to the stage injected into the plants (IP), where the mean reproduction factor (RF) values are determined for each genotype. Genotypes were considered resistant if they showed an RF<1. All genotypes that exhibited an RF>1 were considered susceptible.

The Cucurbitaceae genotypes that were resistant to M. incognita were utilized as rootstocks for the net melon 'Bonus no. 2'.

Cleft grafting was used as described by Yamakawa (1982), because according to Choe (1989), cleft grafting can promote to the seedlings a graft take rate of up to 93%. After grafting, the seedlings were placed in a humid room until local healing, when the percentage of graft take was evaluated.



Based on the reproduction factor (Table 1), only 25% of the treatments were shown to be resistant. The genotypes Luffa cylindrica, pumpkin 'Goianinha', pumpkin 'Mini Paulista', melon 'Redondo Amarelo' and watermelon 'Charleston Gray' showed a reproduction factor (FR) <1, being 0.67, 0.59, 0.32, 0.34 and 0.24, respectively, thereby allowing them to be considered resistant to M. incognita.

All the other genotypes evaluated, such as the hybrid Bonus no.2, showed a reproduction factor >1, being considered susceptible to M. incognita. Cucumber 'Rubi' showed the highest reproduction factor of 6.26, followed by the squash Coroa with 5.12.

Among the rootstocks considered resistant, grafting compatibility between them and the scion (melon 'Bonus no. 2') can be seen in Figure 1, where melon 'Redondo Amarelo' showed the highest graft take rate, at 100%.

After melon 'Redondo Amarelo', the pumpkin 'Mini-Paulista' had a graft take percentage of 94%, also showing good compatibility between scion and rootstock. Therefore, these two rootstocks appear to be very compatible with netmelon 'Bonus no. 2'.

The rootstocks Sponge gourd, watermelon 'Charleston Gray' and pumpkin 'Goianinha' had low graft take percentages: 66%, 62% and 50%, respectively, showing that, despite being resistant to M. incognita, they would not be so interesting for use as rootstocks.



There are numerous studies with rootstocks aimed to achieving resistance to M. incognita. Despite the large diversity among the Cucurbitaceae family, which include 118 genera and 825 species, only 23 species are cultivated as vegetables in many regions of the world (Almeida, 2002). Thus, it is difficult to compare studies that utilize the same species as possible rootstocks.

Singuenza et al. (2005) demonstrated the possibility of using Cucumis metuliferus as rootstock for melon in the control of M. incognita. Xingfang et al. (2006) used Sicyos angulatus L. as rootstock for cucumber in soils with M. incognita, observing little effect on the height of the plant and taste of fruits. Chandra et al. (2010) evaluated the pathogenic potential of M. incoginta in four species of Cucurbitaceae: Lagenaria siceraria, Cucumis sativus, Momordica charantia and Cucurbita pepo, and all were highly or moderately susceptible to the phytonematode, which limited the water and nutrients translocation in the plant.

Santos et al. (1999), working with another variable of resistance to nematodes in which grades were attributed based on the presence or absence of galls, evaluated 54 experimental genotypes of melon regarding to M. incognita resistance and only two of them were considered resistant, while the others were considered moderately resistant, susceptible and highly susceptible. In the present study, the melon 'Redondo Amarelo' was considered resistant, however, differently from observed by Santos et al. (1999) this melon is commercial and not a genotype in study.

In the present study, it was not possible to count galls, since the roots showed high infestation and the galls were almost invisible to naked eyes, making it difficult to count them. Therefore, the parameter used was the reproduction factor described by Oostenbrink (1966).

The eradication of the gall nematode from infested areas is extremely difficult, and the most efficient control measures are the preventive ones, thus, the utilization of resistant rootstocks would be a short-term solution in the control of phytonematodes in infested soils.

According to Gonzáles (1999), compatibility is defined as the capacity of two different plants, united by grafting, to live together as a single plant. We observed that among the rootstocks studied with regard to compatibility, melon 'Redondo Amarelo' showed practically 100% of graft take, showing good botanical affinity between the rootstock and scion.

Similarly to the findings of Ito et al. (2009), the pumpkin 'Mini-paulista' showed about 90% of graft take, which can be explained by the fact that good botanical affinity displayed by the species belonging to the Cucurbitaceae family is related to the continuity of the cambium, since this continuity is crucial for grafting success.

Watermelon 'Charleston Gray' and Sponge gourd showed practically the same performance with about 60 and 65% of graft take, respectively. Rizzo et al. (2000), utilizing the open cleft grafting method, also obtained similar results for Sponge gourd, demonstrating that this rootstock is not so interesting compared to the two rootstocks above mentioned.

Unlike that observed by Ito et al. (2009) and similar to the results obtained by Rizzo et al. (2000), pumpkin 'Goianinha' had the smallest percentage of graft take, with approximately 50%, demonstrating little compatibility with Bonus no. 2. This low graft take rate can be explained by the difference on growth between rootstock and scion, which were planted on the same day: the pumpkin had a more vigorous growth compared to 'Bonus no. 2'.

Therefore, it can be seen that the level of compatibility between scion and rootstock determines the success or failure of the grafting, not considering factors such as temperature and relative humidity during and after grafting, as well as contact surface and salinity, which could have a negative influence on wound healing (callus formation). Poor wound healing can result in reduction of leaves, slow growth and low survival rate of seedlings (Oda et al., 2005; Johkan et al., 2009).

Thus, the movement of water and translocation of nutrients can be determined by the vascular connection or continuity of the cambium between scion and rootstock, thereby affecting other physiological characteristics.



The genotypes Sponge gourd, pumpkin 'Goianinha', pumpkin 'Mini-Paulista', melon 'Redondo Amarelo' and watermelon 'Charleston Gray' are resistant to the nematode M. incognita. The better compatibilities occurred with the rootstocks melon 'Redondo Amarelo', which had a 100% of graft take, followed by the pumpkin 'Mini-Paulista' with 94%. The rootstocks Luffa cylindrica, watermelon 'Charleston Gray' and pumpkin 'Goianinha' had low graft take percentages of 66%, 62% and 50%, respectively.



To FAPESP, for granting a doctoral fellowship to the second author of the work (Process No. 09/52681-6) and an aid fellowship to the fourth author (Process No. 09/52682-2).

To CNPq, for the productivity fellowship awarded to the fourth author (301667/2010-1).



Almeida DPF (2002) Argumentos a favor da utilização do termo "olericultura". Porto, Associação Portuguesa de Horticultura. 4p. (Boletim técnico, 70).         [ Links ]

Chandra P, Sao R, Gautam SK & Poddar AN (2010) Initial Population Density and its Effect on the Pathogenic Potential and Population Growth of the Root Knot Nematode Meloidogyne incognita in Four Species of Cucurbits. Asian Journal of Plant Pathology, 1:1-15.         [ Links ]

Choe JS (1989) Phytophthora blight of green pepper in Korea. Food Fertilizer Technology Center. p.18-25. (Extension Bulletin, 302).         [ Links ]

Costa ND, Grangeiro LC, Faria CMB, Tavares SCH, Alencar JÁ & Araújo JLP (2002) A cultura do melão. Brasília, Embrapa. p.114.         [ Links ]

Factor TL, Araújo JAC & Araújo JPC (2000) Produção de melão rendilhado em ambiente protegido, inverno-primavera, na região de Jaboticabal- SP. Horticultura Brasileira, 18:201-202.         [ Links ]

Gonzáles J (1999) El injerto em hortalizas. In: Vilarbau A & Gonzáles J (Ed.) Planteles: semilleros, viveros. Reus, Ediciones de Horticultura. p.121-128.         [ Links ]

Goto R, Cañizares KAL & Stripari PC (2003) Fatores que influenciam a enxertia. In: Goto R, Santos HS & Cañizares KAL (Eds.) Enxertia em hortaliças. São Paulo, UNESP. p.25-31.         [ Links ]

Ito LA, Charlo HCO, Castoldi R, Braz LT & Camargo M (2009) Seleção de porta-enxertos resistentes ao cancro da haste e seus efeitos na produtividade de melão 'Bônus n° 2'. Revisa Brasileira de Fruticultura, 31:262-267.         [ Links ]

Johkan M, Mitukuri K, Yamasaki S, Mori G & Oda M (2009) Causes of defolation and low survival rate of grafted sweet pepper plants. Scientia Horticulturae, 119:103-107.         [ Links ]

Martínez-Ballesta MC, Alcaraz-López BM, Mota-Cadenas C & Carvajal M (2010) Physiological aspects of rootstock-scion interactions. Scientia Horticulturae, 127:112-118.         [ Links ]

Oda M, Maruyama M & Mori G (2005) Water transfer at graft union of tomato plants grafted onto Solanum rootstocks. Journal of the Japanese Society for Horticultural Science, 74:458-463.         [ Links ]

Oostenbrink M (1966) Major characteristics of the relation between nematodes and plants. Mendelingen Landbouwhogeschool, 66:1-46.         [ Links ]

Peil RM (2003) A enxertia na produção de mudas de hortaliças. Ciência Rural, 33:1169-1177.         [ Links ]

Rizzo AAN, Chaves FCM, Laura VA & Goto R (2000) Avaliação de tipos de enxertia e porta-enxertos para melão rendilhado. Horticultura Brasileira, 18:466-467.         [ Links ]

Rizzo AAN & Braz LT (2001) Características de cultivares de melão rendilhado cultivadas em casa de vegetação. Horticultura Brasileira, 19:370-373.         [ Links ]

Roberts PA, Mathews WC & Veremis JC (1998) Genetic mechanisms of host plant resistance to nematodes. In: Barker KR, Pederson GA & Windham GL (Eds.) Plant and nematode interactions. Madson, American Society of Agronomy Inc. p.209-238.         [ Links ]

Santos AA, Vidal JC, Freire FCO, Paiva WO & Freitas ASM (1999) Avaliação de genótipos de melão para resistência a Meloidoginose e ao oídio. Fortaleza, EMBRAPA-CNPAT. 3p. (Pesquisa em andamento, 55).         [ Links ]

Singuenza C, Schochow M, Turini T & Ploeg A (2005) Use of Cucumis metuliferus as a rootstock for melon to manage Meloidogyne incognita. Journal of Nematology, 37:276-280.         [ Links ]

XingFang G, ShengPing Z & Siyuan Z (2006) The screening of cucumber rootstocks resistant to southern root-knot nematode. China Vegetables, 2:4-8.         [ Links ]

Yamakawa K (1982) Use of rootstocks in solanaceous fruit-vegetable production in Japan. Japanese Agricultural Research Quaterly, 15:175-179.         [ Links ]

Zitter TA, Hopkins DL & Thomas CE (1996) Compendium of cucurbit diseases. Saint. Paul, APS Press. p.27.         [ Links ]



Received: 16/05/2012
Accepted: 29/05/2013

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License