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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.29 no.1 São Paulo Jan./Feb. 1987

http://dx.doi.org/10.1590/S0036-46651987000100005 

ARTIGOS ORIGINAIS

 

Replicação de sendai virus em células epiteliais primárias de camundongo(1)

 

Sendai vinis replication in primary epithelial cells of mice

 

 

Luis I. B. Kanzaki

Instituto Evandro Chagas, Fundação Serviços de Saúde Pública. Av. Almirante Barroso, 492, Caixa Postal 621. CEP 66000 Belém, Pará, Brasil

 

 


RESUMO

Células epiteliais primárias obtidas do trato respiratório de camundongos jovens foram infectadas com o Vírus Hemaglutinante do Japão (HVJ, Sendai Virus) e, a progénie viral, tratada ou não com tripsina foi titulada através do método de Imunofluorescência Indireta. A progénie de Sendai Virus obtida de células epiteliais primárias de camundongo apresentou um título considerável, demonstrando-se que há ativação das partículas virais, capazes de infectar células LLC-MK 2, nas quais, a progénie viral foi titulada.

Unitermos: Sendai virus; Virus hemaglutinante do Japão.


SUMMARY

The Epithelial primary cells harvested from the respiratory tract of young mice were infected with HVJ (Hemagglutinating Virus of Japan, Sendai Virus) and, the progeny virus, trypsin treated or not, were titrated by the Indirect Immunofluorescent Technique. The progeny virus of HVJ obtained from mouse epithelial primary cells presented a considerable titer, demonstrating that the viral particles are activated, being capable of infecting LLCMK-2 cells, in wich, the progeny virus were titrated.


 

 

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

 

 

AGRADECIMENTOS

Agradecemos ao Chefe de Departamento de Microbiologia, Dr. Morio Homma e ao Professor Hakku Hotta na orientação científica e redação original deste trabalho.

 

REFERÊNCIAS BIBLIOGRÁFICAS

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2.  GILBERT, S. F. MIGEON, B. R. - D-Valine as a selective agent for normal human and rodent epithelial cells in culture. Cell, 5: 11-14, 1975.         [ Links ]

3.  HOMMA, M. TASHIRO, M. - Evidence of proteolytic activation of Sendai virus in mouse lung. Arch. Virol., 77: 127-137, 1983.         [ Links ]

4.  HOMMA, M. - Trypsin action on the growth of Sendai virus in tissue culture cells. I. Restoration of the infectivity for L cells by direct action of trypsin on L cell borne Sendai virus. J. Virol., 8: 619-629, 1971.         [ Links ]

5.  HOMMA, M. - Trypsin action on the growth of Sendai virus in tissue culture cells. II. Restoration of the hemolytic activity of L cell borne Sendai virus by trypsin. J. Virol., 9: 829-835, 1972.         [ Links ]

6.  HOMMA, M. OHUCHI, M. - Trypsin action on the growth of Sendai virus in tissue culture cells. III. Structural difference of Sendai viruses grown in eggs and tissue culture cells. J. Virol., 12: 1457-1465, 1973.         [ Links ]

7.  HOSAKA, Y.; SEMBA, T. FUKAI, K. - Artificial assembly of envelope particles of HVJ (Sendai Virus). Fusion activity of envelope particles. J. gen. Virol., 25: 391-404, 1974.         [ Links ]

8.  HOSAKA, Y. HOSOI, J. - Study of negatively stained images of Sendai virus nucleocapsids using minimum-dose system. J. Ultrastruct. Res., 84: 140-150, 1983.         [ Links ]

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11.  NAGATA, I.; MAENO, K.; YOSHII, S. MATSUMOTO, T. - Plaque formation by HVJ in calf kidney cells. Arch. ges. Virusforsch., 15: 257-259, 1965.         [ Links ]

12.  OGURA, H.; SATO, H.; TANAKA, J.; KAMIYAMA, S.; YOSHIE, T. HATANO, M. - Synthesis of M protein of HVJ (Senda virus) in rat glial cells in selectively restricted at a non-permissive temperature. J. gen. Virol., 65: 639-643, 1984.         [ Links ]

13.  OHUCHI, M. HOMMA, M. - Trypsin action on the growth of Sendai virus in tissue culture cells. IV. Evidence for activation of Sendai virus by cleavage of a glycoprotein. J. Virol., 18: 1147-1150, 1976.         [ Links ]

14.  SHIGETA, S. - Plaque formation and growth characteristics of Sendai virus in chicken kidney cell cultures. Tohoku J. exp. Med., 83: 114-120, 1964.         [ Links ]

15.  SUGAWARA, K.; TASHIRO, M. HOMMA, M. - Intermolecular association of HANA glycoprotein of Sendai virus in relation to the expression of biological activities. Virology, 117: 444-455, 1982.         [ Links ]

16.  TASHIRO, M. HOMMA, M. - Pneumotropism of Sendai virus in relation to protease. Mediated activation in mouse lungs. Infect. Immun., 39: 879-888, 1983        [ Links ]

 

 

Recebido para publicação em 4/3/1986.

 

 

(1)  Este trabalho foi realizado na Universidade de Kobe, Faculdade de Medicina, Departamento de Microbiologia sob os auspícios do Governo Japonês (bolsa de estudos), Ministério de Educação, Ciência e Cultura, no período de 1983/85.

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