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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.33 no.5 São Paulo Sept./Oct. 1991

http://dx.doi.org/10.1590/S0036-46651991000500009 

IMMUNOLOGY

 

Isolation and characterization of partially purified leptospiral antigens

 

Isolamento e caracterização de antígenos parcialmente purificados de Leptospira

 

 

M.C. KouryI; H.A. RangelII

IDepartment of Microbiology, ICB/UFMg, CP 24S6, CEP 30161 Belo Horizonte, MG, Brazil
IIDepartment of Microbiology and Immunology, Unicamp, Campinas, Brazil

 

 


SUMMARY

The methanol extract of Leptospira interrogans serovar canicola was purified by precipitation with acetone or acetone and chloroform. The antigenicity of the antigen was not altered by heating or treatment with pepsin and pronase. However the antigenicity was lost when the antigen was treated with periodic acid. Chemical analysis revealed the presence of 40% carbohydrate (22% methylpentose, 28%; hexoses),4% protein, 20% lipid and 2,7% phosphate. The complement fixation test with sera from patients with leptospirosis agreed with the microscopic agglutination reaction.

Key words: Leptospira; Antigens; Purification; Diagnosis.


RESUMO

Extrato metílico de Leptospira interrogans sorovar canicola foi purificado por precipitação com acetona ou acetona e clorofórmio. A antigenicidade não foi alterada por aquecimento ou tratamento com pepsina e pronase, entretanto foi perdida quando o antígeno foi tratado com ácido periódico. Análise química revelou a presença de 40% de carboidrato (22% de metilpentose, 28% de hexose), 4% de proteína, 20% de lípide e 2,7% de fosfato. Reação de fixação de complemento realizada com soros de pacientes com leptospirose apresentou concordância com a reação de aglutinação microscópica.


 

 

ACKNOWLEDGEMENTS

M.C. Koury wishes to acknowledge financial support from FINEP. The authors would like to thank Dr. Enio Cardillo Vieira (ICB/UFMG) for critical evaluation of the manuscript.

 

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Recebido para publicação em 08/03/1991.
Aceito para publicação em 29/07/1991
This work was supported by FINEP.

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