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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.82 no.1 Rio de Janeiro Jan./Mar. 1987

https://doi.org/10.1590/S0074-02761987000100014 

Evaluation of a monoclonal antibody affinity purified antigen for zymodeme specific serological diagnosis of Trypanosoma cruzi infection

Mauro Schechter1 

London School of Hygiene and Tropical Medicine, Department of Medical Protozoology, Wolfson Molecular Biology Unit, London, s.p


ABSTRACT

Theoretically, serological assays with affinity purified marker antigens can allow strain-specific diagnosis even when parasites cannot be retrieved from and infected host. A Trypanosoma cruzi antigen was purified by affinity chromatography using a zymodeme (Z) 2 specific monoclonal antibody (2E2C11). An indirect enzyme-linked immunosorbent assay (ELISA) based on the purified antigen could discriminate between sera from rabbits immunized with T. cruzi zymodeme clones but could not discriminate between sera from mice infected with different zymodemes.

RESUMO

Teoricamente, testes sorológicos que utilizem antígenos purificados por cromatografia de afinidade podem permitir diagnóstico cepaespecífico mesmo quando parasitas não são isoláveis de hospedeiro infectado. Um antígeno de Trypanosoma cruzi foi purificado por cromatografia de afinidade utilizando anticorpo monoclonal (2E2C11) zimodema (Z) específico. Um teste ELISA baeado em tal antígeno mostrou ser capaz de discriminar soros de coelhos imunizados com clones representativos de diferentes zimodemas. No entando, quando soros de camundongos infectados foram analisados, tal teste mostrou-se incapaz de discriminação.

 

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