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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.88 no.3 Rio de Janeiro July/Sept. 1993 

Standardization of serological tests for detecting anti-Trypanosoma cruzi antibodies in dogs

M. A. Lauricella1 

C. Wisnivesky-Colli2 

R. Gürtler2 

R. Petersen2 

M. Bujas3 

E. L. Segura1 

Instituto Nacional de Chagas Dr M Fatala Chabén, Departamento de investigación, Buenos Aires, Argentina

Universidad de Buenos Aires, Facultad de Ciencias Exáctas y Naturales, Departamento de Ciencias Biológicas. Unidad Ecologia de Reservórios y Vectores de Parásitos, Buenos Aires, Argentina

Hospital Italiano, Servicio de Hemoterapia, Buenos Aires, Argentina


This paper reports on the standardization of four serological reactions currently used in human serodiagnosis for the detection of anti-Trypanosoma cruzi antibodies in naturally and experimentally infected dogs. Indirect immunofluorescence test (IFAT) and hemagglutination test (IHAT) were standardized, and complement fixation test (CFT) and direct agglutination test (DAT) were used for diagnostic confirmation. Four hundred and eighty one mongrel dogs that were studied by xenodiagnosis were used: (1) parasitemic dogs of two localities of endemic area (EA) of Santiago del Estero province in Argentina (n = 134); (2) non-parasitemic dogs of the same area (n = 285); (3) dogs experimentally infected with T. cruzi in the patent period (n = 6); (4) non-infected dogs (n = 56) which were born in the city of Buenos Aires (BA), one non-EA for Chagas' disease. For IFAT, parasitemic dogs EA showed 95% of reactive sera. Non parasitemic dogs EA showed 77% of non reactive sera. None sera from BA were reactive for dilutions higher than four. For IHAT, 84% of sera of parasitemic dogs EA showed serological reactivity and among non parasitemic dogs BA, 61% were non reactive, while the remainder showed at most titres of 1/16. The cut-off titres for IFAT and IHAT were 1/16 and 1/32 respectively, and for CFT and DAT 1/1 and 1/128 respectively. Sensitivity for IFAT, IHAT, CF and DAT were 95%, 84%, 97% and 95% respectively.


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